Biological Resource Center, NITE (NBRC)

Anaerolinea thermophila UNI-1T(= NBRC 100420T)

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Members of the phylum Chloroflexi represent a wide variety of phenotypes, and distributed widely throughout the environment. The phylum Chloroflexi is currently divided into at least five classes based on 16S rRNA genes.
Anaerolinea thermophila UNI-1T(= NBRC 100420T)belongs to the class Anaerolineae containing a number of diverse environmental 16S rRNA gene sequences with only a few cultured strains. A. thermophila UNI-1T is the first cultivated strain in this class.

A. thermophila UNI-1T was isolated from a thermophilic upflow anaerobic sludge blanket reactor treating fried soybean-curd manufacturing waste water. A. thermophila UNI-1T is a strictly anaerobic, filamentous (longer than 100 ㎛ and 0.2 to 0.3 ㎛) bacterium that grows chemo-organotrophically on a number of carbohydrates and amino acids in the presence of yeast extract.

The genome of A. thermophila UNI-1T consisted of a single circular chromosome (3,532,378 bp; G+C content of 53.8%) containing 3,179 predicted protein-coding genes. The reconstructed central metabolic pathways from the A. thermophila UNI-1T genome showed that a lot of biosynthesis pathways whose products are essential for growth, such as nucleotide, methionine, fatty acid, vitamin and coenzyme seemed to be incomplete. Remarkably, the biosynthesis pathway of fatty acid seemed to lack genes necessary for the chain elongation. On the other hand, the genome encodes unique genes and pathways which have been mainly described for archaea. These analysis data would provide insights into the understanding of Anaerolineae species and their ecological environment.

Anaerolinea thermophila
Courtesy of
Dr. Sekiguchi (AIST)

References:

[1] Syntrophothermus lipocalidus gen. nov., sp. nov., a novel thermophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate.
Sekiguchi, Y., Kamagata, Y., Nakamura, K., Ohashi, A., Harada, H. (2000)
Int. J. Syst. Evol. Microbiol. 50:771-779. [PMID:10758888]
[2] In situ detection, isolation, and physiological properties of a thin filamentous microorganism abundant in methanogenic granular sludges: a novel isolate affiliated with a clone cluster, the green non-sulfur bacteria, subdivision I.
Sekiguchi, Y., Takahashi, H., Kamagata, Y., Ohashi, A., Harada, H. (2001)
Appl. Environ. Microbiol. 67:5740-5749 [PMID:11722931]
[3] Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level.
Sekiguchi, Y., Yamada, T., Hanada, S., Ohashi, A., Harada, H., Kamagata, Y. (2003)
Int. J. Syst. Evol. Microbiol. 53:1843-1851 [PMID:14657113]
[4] Characterization of filamentous bacteria, belonging to candidate phylum KSB3, that are associated with bulking in methanogenic granular sludges.
Yamada T, Yamauchi T, Shiraishi K, Hugenholtz P, Ohashi A, Harada H, Kamagata Y, Nakamura K, Sekiguchi Y.(2007)
ISME J. 3:246-255 [PMID:18043635]
Genomic size 3,532,378 bp
The number of ORFs 3,177
GC content 53.85 %
Genome Database DOGAN
NBRC No. 100420
*:
NBRC is the acronym for "the NITE Biological Resource Center".
The URL of NBRC is http://www.nbrc.nite.go.jp/e/index.html.
Distribution of Our Microbial Genomic DNAs
At the Biological Resource Center of the National Institute of Technology and Evaluation (NITE Biological Resource Center, an Incorporated Administrative Agency), we have been distributing the microbial genomic DNA.

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