Biological Resource Center, NITE (NBRC)

The outline of Tetragenococcus halophilus NBRC 12172 proteome

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We have analyzed the proteome of Tetragenococcus halophilus NBRC 12172 cultured with added salt concentration of 0% and 10% by using the following methods.

  1. (1) Soluble fraction: Two-dimensional PAGE followed by MALDI-TOFMS
  2. (2) Soluble fraction: One-dimensional SDS-PAGE in combination with two-dimensional LC-MS/MS
  3. (3) Soluble fraction: Whole shotgun proteomics
  4. (4) Membrane enriched fraction (treated by lysozyme and ultracentrifuged): One-dimensional SDS-PAGE in combination with two-dimensional LC-MS/MS
  5. (5) Membrane enriched fraction (disrupted on dry ice and ultracentrifuged): on-column digestion followed by LC/MS/MS
  6. (6) Membrane enriched fraction (disrupted on dry ice): on-column digestion followed by LC/MS/MS

N-terminal amino acid sequencing by the protein sequencer were also performed.

The result of proteome analysis

Tetragenococcus halophilus NBRC 12172 cultured under 0% salt condition was in more stressed condition rather than in 10% condition. It indicated that this strain acquires energy using high salt condition environment. In addition, it was suggested that the salt resistance was related to uptake of osmoregulation substance and membrane glycoprotein.

Tetragenococcus halophilus NBRC 12172 proteome data in DOGAN

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