Chemical Name：[4-{Bis(4-dimethylaminophenyl)methylene}-2,5-cyclohexadien-1-ylidene]dimethylammonium chloride; C.I. Basic Violet 3
CAS：548-62-9

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
1	Acute toxicity （Oral）	Category 4		Warning	H302: Harmful if swallowed	P301+P312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. P264: Wash ... thoroughly after handling. P270: Do not eat, drink or smoke when using this product. P330: Rinse mouth. P501: Dispose of contents/container to ...	Since rat LD50 is 420 mg/kg (RTECS, accessed in September 2007), the substance was classified into Category 4.
1	Acute toxicity （Dermal）	Classification not possible	-	-	-	-	No data available.
1	Acute toxicity （Inhalation: Gases）	Not applicable	-	-	-	-	Since the substance is a solid (GHS definition), it was classified into the "Not applicable" category.
1	Acute toxicity （Inhalation: Vapours）	Classification not possible	-	-	-	-	No data available.
1	Acute toxicity （Inhalation: Dusts and mists）	Classification not possible	-	-	-	-	No data available.
2	Skin corrosion/irritation	Classification not possible	-	-	-	-	No data available.
3	Serious eye damage/eye irritation	Classification not possible	-	-	-	-	No data available.
4	Respiratory sensitization	Classification not possible	-	-	-	-	No data available.
4	Skin sensitization	Classification not possible	-	-	-	-	No data available.
5	Germ cell mutagenicity	Classification not possible	-	-	-	-	Due to lack of appropriate test results in in-vivo mutagenicity/genotoxicity studies, classification is not possible. Regarding in vitro studies, chromosomal aberration tests using CHO cells gave negative results while Ames test produced equivocal results (NTP DB (accessed in September 2007)).
6	Carcinogenicity	Category 2		Warning	H351: Suspected of causing cancer	P308+P313: IF exposed or concerned: Get medical advice/attention. P201: Obtain special instructions before use. P202: Do not handle until all safety precautions have been read and understood. P281: Use personal protective equipment as required. P405: Store locked up. P501: Dispose of contents/container to ...	It is rated as Carc. 3 by the EU (2007). In addition, in mouse carcinogenicity tests, increased incidences of hepatocellular carcinoma were noted for both sexes, and in rat carcinogenicity tests, increased incidences of follicular cell adenocarcinoma of the thyroid gland were observed in both sexes (HSDB, 2003). Based on the information above, the substance was classified into Category 2.
7	Reproductive toxicity	Category 2		Warning	H361: Suspected of damaging fertility or the unborn child	P308+P313: IF exposed or concerned: Get medical advice/attention. P201: Obtain special instructions before use. P202: Do not handle until all safety precautions have been read and understood. P281: Use personal protective equipment as required. P405: Store locked up. P501: Dispose of contents/container to ...	According to NTP DB (accessed in September 2007), in forced oral administration test using pregnant rats, malformations including hydronephrosis, hydroureter, and short ribs were observed in fetuses at doses that induce maternal toxicity. Based on these reports, the substance was classified into Category 2.
8	Specific target organ toxicity - Single exposure	Classification not possible	-	-	-	-	No data available.
9	Specific target organ toxicity - Repeated exposure	Category 2 (digestive system, systemic toxicity)		Warning	H373: May cause damage to organs through prolonged or repeated exposure (digestive system, systemic toxicity)	P260: Do not breathe dust/fume/gas/mist/vapours/spray. P314: Get medical advice/attention if you feel unwell. P501: Dispose of contents/container to ...	In oral administration test with rabbits, labored breathing, cyanosis, diarrhea were noted (RTECS, accessed in September 2007). In light of this report, we considered the digestive system/systemic toxicity to be the target organs. Although these symptoms occurred at doses that fall under Category 1, these results are reported in Priority 2 evaluation document, which lists Category 2 outcomes. Thus, the substance was classified into Category 2 for the digestive system/systemic toxicity.
10	Aspiration hazard	Classification not possible	-	-	-	-	No data available.