Chemical Name：Sodium 3,3'-[(1,1'-biphenyl-4,4'-diyl)bis(azo)]bis(4-aminonaphthalene-1-sulfonate); C.I. Direct Red 28
CAS：573-58-0

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
1	Acute toxicity （Oral）	Not classified	-	-	-	-	Since the LD50 is 15.2 g/kg for female and male rats (HSDB, 2003), the substance was classified into the "Not classified" category.
1	Acute toxicity （Dermal）	Not classified	-	-	-	-	Since the LD50 is >= 8.0 g/kg for rabbits (HSDB, 2003), the substance was classified into the "Not classified" category.
1	Acute toxicity （Inhalation: Gases）	Not applicable	-	-	-	-	Solid (GHS definition)
1	Acute toxicity （Inhalation: Vapours）	Classification not possible	-	-	-	-	No data available.
1	Acute toxicity （Inhalation: Dusts and mists）	Not classified	-	-	-	-	The rat LCLo (1 hour) is 50 g/m3 (RTECS, accessed in October 2007), and the 4-hour conversion value of the rat LCLo = (50 g/m3) /4 = 12.5 g/m3 = 12.5 mg/L. Since the LC50 is estimated to be > 12.5 mg/L, the substance was classified into the "Not classified" category using classification criteria for dust.
2	Skin corrosion/irritation	Classification not possible	-	-	-	-	It is reported to be a possible irritant (HSFS, 1999), but details of the study including evidence that supports the conclusion are not provided. Thus, classification is not possible due to insufficient test results available.
3	Serious eye damage/eye irritation	Category 2A		Warning	H319: Causes serious eye irritation	P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337+P313: If eye irritation persists: Get medical advice/attention. P264: Wash ... thoroughly after handling. P280: Wear protective gloves/protective clothing/eye protection/face protection.	Based on the report that a 100 mg application of the substance to rabbits induced moderate irritation (RTECS, accessed in October 2007), the substance was classified into Category 2A.
4	Respiratory sensitization	Classification not possible	-	-	-	-	It may cause severe allergic reaction (anaphylactic shock) (HSDB, 2003). However, due to insufficient data available, classification is not possible.
4	Skin sensitization	Classification not possible	-	-	-	-	It may cause severe allergic reaction (anaphylactic shock) (HSDB, 2003). However, due to insufficient data available, classification is not possible.
5	Germ cell mutagenicity	Classification not possible	-	-	-	-	The following reports are available: application of the substance onto the skin of mice was associated with DNA adduct formation; reverse mutation test using Salmonella typhimurium gave positive results; and UDS test using rat hepatocytes yielded positive results (RTECS, accessed in October 2007). However, since no other in vivo test results are obtainable, classification is not possible due to insufficient test result available.
6	Carcinogenicity	Classification not possible	-	-	-	-	It is rated as Cat. 2 by the EU (2007), but no details such as rationale for the classification are provided. Thus, classification is not possible.
7	Reproductive toxicity	Category 2		Warning	H361: Suspected of damaging fertility or the unborn child	P308+P313: IF exposed or concerned: Get medical advice/attention. P201: Obtain special instructions before use. P202: Do not handle until all safety precautions have been read and understood. P281: Use personal protective equipment as required. P405: Store locked up. P501: Dispose of contents/container to ...	Oral administration of the substance in mice on Gestational Days 8-12 was associated with inhibition of testicular and ovarian function in the offspring. In addition, female offspring from the substance-exposed dams produced less litters than that from untreated dams (HSDB (2003)). Based on these reports, the substance was classified into Category 2.
8	Specific target organ toxicity - Single exposure	Classification not possible	-	-	-	-	In a test in which rats underwent 1-hour inhalation exposure to a dust form of this substance, gross necropsy revealed hemorrhaging of the lungs in decedents, and pulmonary and adrenal congestion in survivors (HSDB, 2003). However, exposure concentrations associated with these symptoms are above the range of Category 2 guidance values. As such, classification is not possible.
9	Specific target organ toxicity - Repeated exposure	Classification not possible	-	-	-	-	No data available.
10	Aspiration hazard	Classification not possible	-	-	-	-	No data available.