Chemical Name：coumaphos (ISO); O-3-chloro-4-methylcoumarin-7-yl O,O-diethyl phosphorothioate
CAS：56-72-4

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
1	Acute toxicity （Oral）	Category 2		Danger	H300: Fatal if swallowed	P301+P310: IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. P264: Wash ... thoroughly after handling. P270: Do not eat, drink or smoke when using this product. P321: Specific treatment （see ... on this label）. P330: Rinse mouth. P405: Store locked up. P501: Dispose of contents/container to ...	In all of the tests documented, the rat LD50 values were lower in females than in males. Thus, only female data were evaluated. Within female rats, the range of LD50 values was 13-37mg/kg (ACGIH (2007); PATTY, 5th (2001); JMPR (1990)), which was equivalent to the Category 2 guidance values. Thus, the substance was classified into Category 2.
1	Acute toxicity （Dermal）	Category 3		Danger	H311: Toxic in contact with skin	P302+P352: IF ON SKIN: Wash with plenty of soap and water. P280: Wear protective gloves/protective clothing/eye protection/face protection. P312: Call a POISON CENTER or doctor/physician if you feel unwell. P322: Specific treatment （see ... on this label）. P361: Remove/Take off immediately all contaminated clothing. P363: Wash contaminated clothing before reuse. P405: Store locked up. P501: Dispose of contents/container to ...	The rat LD50 values documented were 860mg/kg (ACGIH (2007); PATTY, 5th (2001)) and > 2400mg/kg (ACGIH (2007); PATTY, 5th (2001)), and the rabbit LD50 value documented was 500mg/kg (ACGIH (2007)). Based on the rabbit value, which was lower than the rat values, the substance was classified into Category 3.
1	Acute toxicity （Inhalation: Gases）	Not applicable	-	-	-	-	Solid (GHS definition)
1	Acute toxicity （Inhalation: Vapours）	Classification not possible	-	-	-	-	No data available.
1	Acute toxicity （Inhalation: Dusts and mists）	Category 2		Danger	H330: Fatal if inhaled	P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P403+P233: Store in a well-ventilated place. Keep container tightly closed. P260: Do not breathe dust/fume/gas/mist/vapours/spray. P271: Use only outdoors or in a well-ventilated area. P284: Wear respiratory protection. P310: Immediately call a POISON CENTER or doctor/physician. P320: Specific treatment is urgent （see ... on this label）. P405: Store locked up. P501: Dispose of contents/container to ...	Of 1-hour rat LC50 of 1081mg/m3 (male) (PATTY, 5th (2001)) and 341mg/m3 (female) (PATTY, 5th (2001)), the female value, which was lower than the male value, was used to estimate the 4-hour LC50 of 0.08525mg/L. Based on this value, the substance was classified into Category 2.
2	Skin corrosion/irritation	Not classified	-	-	-	-	Based on the description that the substance is not irritating to the skin (ACGIH (2007); PATTY, 5th (2001)), it was classified into "Not classified".
3	Serious eye damage/eye irritation	Category 2B	-	Warning	H320: Causes eye irritation	P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337+P313: If eye irritation persists: Get medical advice/attention. P264: Wash ... thoroughly after handling.	Based on the description that the substance is a "mild eye irritant" (ACGIH (2007); PATTY, 5th (2001)) and it "may cause irritation or burns of the skin, eyes, and mucous membranes," the substance was classified into Category 2B.
4	Respiratory sensitization	Classification not possible	-	-	-	-	No data available.
4	Skin sensitization	Not classified	-	-	-	-	Based on the description that the substance is not a skin sensitizer (ACGIH (2007); PATTY, 5th (2001)), it was classified into "Not classified".
5	Germ cell mutagenicity	Not classified	-	-	-	-	In vivo mutagenicity tests using somatic cells (mouse micronucleus tests) yielded negative results (ACGIH (2007); PATTY, 5th (2001)). Therefore, the substance was classified into "Not classified". In vitro mutagenicity studies using microorganisms also yielded negative results.
6	Carcinogenicity	Not classified	-	-	-	-	According to ACGIH (2007), the substance has been classified into A4. And 2-year carcinogenicity studies with rats and mice both yielded negative results (NTP (1979); ACGIH (2007); PATTY, 5th (2001); JMPR (1990)). Thus, the substance was classified into "Not classified".
7	Reproductive toxicity	Not classified	-	-	-	-	In the 2-generation rat studies, while inhibition of cholinesterase activity was detected, no adverse effects on sexual function, fertility, or development of the offspring were observed (ACGIH (2007); PATTY, 5th (2001)). Similarly, in tests using rats and rabbits during the gestation period, no adverse effects were detected. On the other hand, miscarriages were observed in rabbits when doses lethal to parental animals were administered (ACGIH (2007); PATTY, 5th (2001)). Also, in mice, when doses that severely decreased survival rates of parental animals were administered, a decreased number of pregnancies, decreased litter size, and shortened longevity of the offspring were observed. In other words, these adverse effects observed were associated only with high doses that cause mortality in maternal animals, and thus, they were not considered to be the rational for classification. In conclusion, because reproductive and developmental toxicity was not observed in rats, rabbits, or mice, the substance was classified into "Not classified".
8	Specific target organ toxicity - Single exposure	Category 1 (nervous system)		Danger	H370: Causes damage to organs (nervous system)	P307+P311: IF exposed: Call a POISON CENTER or doctor/physician. P260: Do not breathe dust/fume/gas/mist/vapours/spray. P264: Wash ... thoroughly after handling. P270: Do not eat, drink or smoke when using this product. P321: Specific treatment （see ... on this label）. P405: Store locked up. P501: Dispose of contents/container to ...	Cases of exposure to humans, such as nausea, vomiting, stomachache, sweating, urination, miosis, bronchorrhea, and hypersalivation (ACGIH (2007); PATTY, 5th (2001)), have been reported to be typical symptoms of organophosphate poisoning. Similarly, ICSC and ICSC(J) list convulsions, dizziness, sweating, labored breathing, nausea, unconsciousness, pupillary constriction, muscle cramp, excessive salivation, abdominal cramps, diarrhea, and vomiting, as acute symptoms. In rats, the following have been observed: cholinergic toxicity, delayed neurotoxicity, diarrhea, and decrease in erythrocytes, blood plasma, and cholinesterase activities in the brain. These effects fit Category 1 based on its guidance values (ingestion of <= 250mg/kg in rats). Since the substance induces toxicological symptoms in humans and test animals, and due to its mode of action, the substance was classified into Category 1 (nervous system).
9	Specific target organ toxicity - Repeated exposure	Category 1 (nervous system)		Danger	H372: Cause damage to organs through prolonged or repeated exposure (nervous system)	P260: Do not breathe dust/fume/gas/mist/vapours/spray. P264: Wash ... thoroughly after handling. P270: Do not eat, drink or smoke when using this product. P314: Get medical advice/attention if you feel unwell. P501: Dispose of contents/container to ...	The substance is regarded as cholinesterase inhibitors in humans (ICSC (1997); ICSC(J) (1997)). In rats and dogs, it is documented to cause inhibition of RBC, plasma, the brain, and ocular muscle cholinesterase activity, as well as to induce symptoms of cholinergic toxicity (ACGIH (2007)), and muscle fasciculation and tremors (PATTY, 5th (2001)). These effects on the nervous system were observed at the doses equivalent to the Category 1 guidance values (ingestion of <= 10mg/kg for 90 days or more in rats and dogs). Therefore, the substance was classified into Category 1 (nervous system).
10	Aspiration hazard	Classification not possible	-	-	-	-	No data available.

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
11	Hazardous to the aquatic environment （Acute）	Category 1		Warning	H400: Very toxic to aquatic life	P273: Avoid release to the environment. P391: Collect spillage. P501: Dispose of contents/container to ...	Since 48h-LC50 = 0.001mg/L for crustaceans (Daphnia magna) (HSDB 2003), the substance was classified into Category 1.
11	Hazardous to the aquatic environment （Long-term）	Category 1		Warning	H410: Very toxic to aquatic life with long lasting effects	P273: Avoid release to the environment. P391: Collect spillage. P501: Dispose of contents/container to ...	Since its classification for acute toxicity is Category 1 and it is estimated not to be rapidly degradable (estimated value: SRC: BioWin V4.10), the substance was classified into Category 1.