GHS Classification Result

Chemical Name：1,3-dinitrobenzene
CAS：99-65-0

Result：
ID: 20A2155
Classifier: Ministry of Health, Labour and Welfare （MHLW）, Ministry of the Environment （MOE）
Year Classified: FY2008
Reference Manual: GHS Classification Guidance by the Japanese Government （Sep, 2008）

PHYSICAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Explosives Not classified - - - - It contains chemical groups associated with explosive properties, and its oxygen balance is -952, which is lower than -200. Thus, the substance was classified into the "Not classified" category.
2 Flammable gases （including chemically unstable gases） Not applicable - - - - Solid (GHS definition)
3 Aerosols Not applicable - - - - Not aerosol products.
4 Oxidizing gases Not applicable - - - - Solid (GHS definition)
5 Gases under pressure Not applicable - - - - Solid (GHS definition)
6 Flammable liquids Not applicable - - - - Solid (GHS definition)
7 Flammable solid Classification not possible - - - - No data available.
8 Self-reactive substances and mixtures Type G - - - - It is categorized into the UNRTDG's Class 6 (UN No. 3443).
9 Pyrophoric liquids Not applicable - - - - Solid (GHS definition)
10 Pyrophoric solids Not classified - - - - It is categorized into the UNRTDG's Class 6 (UN No. 3443).
11 Self-heating substances and mixtures Classification not possible - - - - Test methods applicable to solid (melting point <= 140degC) substances are not available.
12 Substances and mixtures which, in contact with water, emit flammable gases Not applicable - - - - The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).
13 Oxidizing liquids Not applicable - - - - Solid (GHS definition)
14 Oxidizing solids Classification not possible - - - - The molecule contains oxygen, which is bonded to elements other than carbon or hydrogen. Due to lack of data on its oxidizing properties, the substance was classified into the "Classification not possible" category.
15 Organic peroxides Not applicable - - - - Organic compounds containing no bivalent -O-O- structure
16 Corrosive to metals Classification not possible - - - - Test methods applicable to solid substances are not available.

HEALTH HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Acute toxicity （Oral） Category 3 Danger H301: Toxic if swallowed P301+P310: IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P321: Specific treatment （see ... on this label）.
P330: Rinse mouth.
P405: Store locked up.
P501: Dispose of contents/container to ... 		Of four rat LD50 values documented: 48 mg/kg, 91 mg/kg (male), 81 mg/kg (female), and 59 mg/kg (ATSDR (1995)), three values fall under Category 3. Thus, the substance was classified into Category 3.
1 Acute toxicity （Dermal） Category 4 Warning H312: Harmful in contact with skin P302+P352: IF ON SKIN: Wash with plenty of soap and water.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P312: Call a POISON CENTER or doctor/physician if you feel unwell.
P322: Specific treatment （see ... on this label）.
P363: Wash contaminated clothing before reuse.
P501: Dispose of contents/container to ... 		Both of two LD50 values documented: 1200 mg/kg for rats (OECD TG 402) (IUCLID (2000)) and 1990 mg/kg for rabbits (HSDB (2005)), fall under Category 4.
1 Acute toxicity （Inhalation: Gases） Not applicable - - - - Solid (GHS definition)
1 Acute toxicity （Inhalation: Vapours） Classification not possible - - - - No data available.
1 Acute toxicity （Inhalation: Dusts and mists） Classification not possible - - - - No data available.
2 Skin corrosion/irritation Not classified - - - - Rabbit tests (OECD TG 404 GLP) found that the substance is not irritating (IUCLID (2000)). Based on this document, the substance was classified into the "Not classified" category.
3 Serious eye damage/eye irritation Category 2 Warning H319: Causes serious eye irritation P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P337+P313: If eye irritation persists: Get medical advice/attention.
P264: Wash ... thoroughly after handling.
P280: Wear protective gloves/protective clothing/eye protection/face protection. 		Rabbit tests found that the substance is mildly irritating (ATSDR (1995)). Based on this result, it was classified into Category 2. In other rabbit tests (OECD TG 405 GLP), the substance was found to be irritating (IUCLID (2000)).
4 Respiratory sensitization Classification not possible - - - - No data available.
4 Skin sensitization Category 1 Warning H317: May cause an allergic skin reaction P302+P352: IF ON SKIN: Wash with plenty of soap and water.
P333+P313: If skin irritation or rash occurs: Get medical advice/attention.
P261: Avoid breathing dust/fume/gas/mist/vapours/spray.
P272: Contaminated work clothing should not be allowed out of the workplace.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P321: Specific treatment （see ... on this label）.
P363: Wash contaminated clothing before reuse.
P501: Dispose of contents/container to ... 		Since guinea pig maximization tests (OECD TG 406 GLP) found that the substance is skin sensitizing (50% positive reaction rate) (IUCLID (2000)), it was classified into Category 1.
5 Germ cell mutagenicity Classification not possible - - - - Classification is not possible due to lack of in vivo test results as well as strong positive results in in vitro mutagenicity tests using several indices. Regarding in vitro mutagenicity tests, choromosomal aberration tests using V 79 cells gave positive results (IUCLID (2000)) and Ames tests also yielded positive results (NTP DB (accessed in September 2008)).
6 Carcinogenicity Classification not possible - - - - The mixture of isomers was rated as Group 2B by the IARC (HSDB (2004)) and Classification D by the EPA (IRIS (2005)). However, due to lack of data on 1,3-dinitrobenzene, classification is not possible.
7 Reproductive toxicity Category 2 Warning H361: Suspected of damaging fertility or the unborn child P308+P313: IF exposed or concerned: Get medical advice/attention.
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P281: Use personal protective equipment as required.
P405: Store locked up.
P501: Dispose of contents/container to ... 		In oral administration tests using female and male rats, effects of administration on fertility, such as loss of coitus ability, diminished sperm production, and decreased cauda epididymal sperm reserves, were noted (BUA Report (1992), HSDB (2005), etc.). Based on these results, the substance was classified into Category 2.
8 Specific target organ toxicity - Single exposure Category 1 (blood system, nervous system) Danger H370: Causes damage to organs (blood system, nervous system) P307+P311: IF exposed: Call a POISON CENTER or doctor/physician.
P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P321: Specific treatment （see ... on this label）.
P405: Store locked up.
P501: Dispose of contents/container to ... 		In forced oral administration tests using rats, presence of methemoglobin (28%) and cyanosis were noted at 25 mg/kg, which falls under Category 1 guidance doses (ATSDR (1995)), and increased erythropoietic activity was detected at 15 mg/kg (ATSDR (1995)). In other forced oral administration tests using rats, ataxia was observed at 48 mg/kg, which falls under Category 1 guidance doses (ATSDR (1995)), and flaccid paralysis was noted at 20 mg/kg (HSDB (2005)). Based on these results, the substance was classified into Category 1 (nervous system and blood system).
9 Specific target organ toxicity - Repeated exposure Category 1 (blood system) Danger H372: Cause damage to organs through prolonged or repeated exposure (blood system) P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P314: Get medical advice/attention if you feel unwell.
P501: Dispose of contents/container to ... 		In 12-week forced oral administration (5 days per week) tests using rats, splenic hemosiderosis (1.5 mg/kg) accompanied with hemolytic anemia were detected at 0.75-6 mg/kg, which fall under Category 1 guidance doses (ATSDR (1995)). Thus, the substance was classified into Category 1 (blood system). Also, there is a report that in rat oral route administration tests, testicular germinal epithelial cells, which had sloughed off the testicular epithelium, were observed in caput epididymal fluid (HSDB (2005)).
10 Aspiration hazard Classification not possible - - - - No data available.

ENVIRONMENTAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
11 Hazardous to the aquatic environment （Acute） Category 1 Warning H400: Very toxic to aquatic life P273: Avoid release to the environment.
P391: Collect spillage.
P501: Dispose of contents/container to ... 		Since its 96-hour EbC50 = 0.24 mg/L for algae (chlorella) (IUCLID, 2000), the substance was classified into Category 1.
11 Hazardous to the aquatic environment （Long-term） Category 1 Warning H410: Very toxic to aquatic life with long lasting effects P273: Avoid release to the environment.
P391: Collect spillage.
P501: Dispose of contents/container to ... 		Since its classification for acute toxicity is Category 1, and it is not rapidly degradable (BIOWIN), the substance was classified into Category 1.


NOTE:
* Classification was conducted by relevant Japanese Ministries in accordance with GHS Classification Guidance for the Japanese Government, and is intended to provide a reference for preparing GHS labelling and SDS for users.
* This is a provisional English translation of classification results and is subject to revision without notice.
* The responsibility for any resulting GHS labelling and SDS referenced from this site is with users.

Reference：
Reference Manual
Definitions / Abbreviations
Model Label by MHLW

 MHLW Website （in Japanese Only）
Model SDS by MHLW

 MHLW Website （in Japanese Only）

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