GHS Classification Result

Chemical Name：methyl acetoacetate
CAS：105-45-3

Result：
ID: 20A2159
Classifier: Ministry of Health, Labour and Welfare （MHLW）, Ministry of the Environment （MOE）
Year Classified: FY2008
Reference Manual: GHS Classification Guidance by the Japanese Government （Sep, 2008）

PHYSICAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Explosives Not applicable - - - - There are no chemical groups associated with explosive properties present in the molecules.
2 Flammable gases （including chemically unstable gases） Not applicable - - - - Liquid (GHS definition)
3 Aerosols Not applicable - - - - Not aerosol products.
4 Oxidizing gases Not applicable - - - - Liquid (GHS definition)
5 Gases under pressure Not applicable - - - - Liquid (GHS definition)
6 Flammable liquids Category 4 - Warning H227: Combustible liquid P370+P378: In case of fire: Use ... for extinction.
P403+P235: Store in a well-ventilated place. Keep cool.
P210: Keep away from heat/sparks/open flames/hot surfaces. ? No smoking.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P501: Dispose of contents/container to ... 		Since its flash point is 67degC (closed cup) (Hommel, 1996), the substance was classified into Category 4.
7 Flammable solid Not applicable - - - - Liquid (GHS definition)
8 Self-reactive substances and mixtures Not applicable - - - - There are no chemical groups present in the molecule associated with explosive or self-reactive properties.
9 Pyrophoric liquids Not classified - - - - Since its autoignition point is 280degC (Chapman ver. 16:2, 2008; Hommel, 1996; HSDB, 2005), which is above 70degC, the substance was classified into the "Not classified" category.
10 Pyrophoric solids Not applicable - - - - Liquid (GHS definition)
11 Self-heating substances and mixtures Classification not possible - - - - Test methods applicable to liquid substances are not available.
12 Substances and mixtures which, in contact with water, emit flammable gases Not applicable - - - - The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).
13 Oxidizing liquids Not applicable - - - - The substance contains oxygen (but not fluorine or chlorine) which is chemically bonded only to carbon or hydrogen.
14 Oxidizing solids Not applicable - - - - Liquid (GHS definition)
15 Organic peroxides Not applicable - - - - Organic compounds containing no bivalent -O-O- structure in the molecule
16 Corrosive to metals Not classified - - - - Based on the description that as durable transport container material, aluminum, high-grade steel, synthetic substances, glasses, or porcelain enamel is used (Hommel, 1996), the substance was classified into the "Not classified" category.

HEALTH HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Acute toxicity （Oral） Not classified - - - - In rat tests (OECD TG 401, GLP), no mortality occurred in 2000 mg/kg groups (Toxicity Testing Reports of Environmental Chemicals (Chemicals Investigation Promoting Council), accessed in September 2008). In other rat tests, LD50 was found to be 2580-3370 mg/kg bw (IUCLID, 2000; HSDB, 2005). Based on these results, the substance was classified into the "Not classified" category using the JIS criteria. In the United Nations classification, it falls under Category 5.
1 Acute toxicity （Dermal） Not classified - - - - Since its rabbit LD50 is > 5000 mg/kg bw (IUCLID, 2000), the substance was classified into the "Not classified" category. The information in PATTY 5th (2001) was not used as grounds for classification because original literature cited there is outdated.
1 Acute toxicity （Inhalation: Gases） Not applicable - - - - Liquid (GHS definition)
1 Acute toxicity （Inhalation: Vapours） Classification not possible - - - - No data available.
1 Acute toxicity （Inhalation: Dusts and mists） Not classified - - - - Its saturated vapour pressure concentration is calculated as 1168 ppm by converting its vapour pressure value of 118 Pa at 25degC (Howard, 1997; HSDB, 2005). Its rat LC50 of > 6.6 mg/L (conversion value: > 1390 ppm), obtained in 4-hour inhalation tests using rats, is greater than its saturated vapour pressure concentration. Thus, the substance was classified as mist into the "Not classified" category using the JIS criteria.
2 Skin corrosion/irritation Not classified - - - - Rabbit tests (OECD TG 404, GLP) (IUCLID, 2000) found that the substance is not irritating. Based on this description, it was classified into the "Not classified" category.
3 Serious eye damage/eye irritation Category 2 Warning H319: Causes serious eye irritation P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P337+P313: If eye irritation persists: Get medical advice/attention.
P264: Wash ... thoroughly after handling.
P280: Wear protective gloves/protective clothing/eye protection/face protection. 		In both of two studies using rabbits (OECD TG 405, GLP and non-GLP studies) (IUCLID, 2000), the substance was found to be irritating. Based on these results and since it is rated as R36 in the EU classification, the substance was classified into Category 2.
4 Respiratory sensitization Classification not possible - - - - No data available.
4 Skin sensitization Classification not possible - - - - A human maximization test (IUCLID, 2000) found that the substance is not sensitizing. Since this is the only tests result available, and there is not sufficient information to carry out classification. Thus, the substance was categorized into "Classification not possible".
5 Germ cell mutagenicity Classification not possible - - - - Several negative results and one positive result have been documented in in-vitro mutagenicity tests (a reverse mutation test using bacteria, a chromosomal aberration test using cultured cells from Chinese hamsters, and a cytogenetic assay using CHO cells) (Toxicity Testing Reports of Environmental Chemicals (Chemicals Investigation Promoting Council), accessed in September 2008; IUCLID, 2000). Since these results are insufficient to carry out classification, the substance was categorized into "Classification not possible".
6 Carcinogenicity Not classified - - - - Two-year oral administration tests using rats or mice (Results from Carcinogenicity Studies (Ministry of Health, Labour and Welfare), 2005) found that no increase in cases of tumorigenesis or tumor-related incidences was detected in either sex. Based on this description, the substance was classified into the "Not classified" category.
7 Reproductive toxicity Classification not possible - - - - In OECD's combined repeated oral administration / reproduction and developmental studies using rats (GLP) (Toxicity Testing Reports of Environmental Chemicals (Chemicals Investigation Promoting Council), accessed in September 2008), no general toxic effects on parental animals, no effects on reproductive functions, or no abnormalities in observations of delivery, nursing, or neonates were noted at the highest test dose of 1000 mg/kg. However, since there are no test results on teratogenicity, the substance was classified into the "Classification not possible" category.
8 Specific target organ toxicity - Single exposure Category 3 （Narcotic effects） Warning H336: May cause drowsiness or dizziness (narcotic effects) P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P403+P233: Store in a well-ventilated place. Keep container tightly closed.
P261: Avoid breathing dust/fume/gas/mist/vapours/spray.
P271: Use only outdoors or in a well-ventilated area.
P312: Call a POISON CENTER or doctor/physician if you feel unwell.
P405: Store locked up.
P501: Dispose of contents/container to ... 		In single oral administration tests using rats (HSDB, 2005), administration of 1.0 and 2.0 g/kg was associated with sluggish movements, and animals returned to normal behavior within 48 hours. In addition, animals dosed at 2.5 to 4.0 g/kg exhibited lethargy, and at 8.0 g/kg animals immediately became comatose. Based on these reports, the substance was classified into Category 3 (narcotic effects).
9 Specific target organ toxicity - Repeated exposure Classification not possible - - - - In OECD's combined repeated oral administration / reproduction and developmental studies using rats (Crj: CD: Sprague-Dawley strain) (GLP) (Toxicity Testing Reports of Environmental Chemicals (Chemicals Investigation Promoting Council), accessed in September 2008), no mortality occurred, no changes in clinical observations were noted, and no differences in body weight and food consumption were detected relative to control in groups treated at 1000 mg/kg, the highest dose tested. In 100 mg/kg groups, one male rat developed spleen hypertrophy and was diagnosed with leukemia. However, there is a report that leukemia is a spontaneously generated pathological change seen in rats of the same strain and same age by week (C.B. Richter, Laboratory Investigation, 26,419 (1972)), indicating that the change was unrelated to the test substance administered. Also, in 1000 mg/kg groups, one rat showed infiltration of lymphocytes under the left ventricular endocardium, but it occurred at a very low frequency, and there is a report that this pathological change is also attributed to spontaneous generation in rats (I. Narama. 1992. Toxicological study Lecture 5. In: A. Maekawa and Y. Hayashi (eds.), Toxicological pathology. Chijinshokan, Tokyo. p.59.), indicating that the change was unrelated to the test substance administered. Thus, regarding oral administration, the substance falls under the "Not classified" category. However, since data on other administration routes are not available, the substance was classified into the "Classification not possible" category.
10 Aspiration hazard Classification not possible - - - - No data available.

ENVIRONMENTAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
11 Hazardous to the aquatic environment （Acute） Not classified - - - - Its 48-hour EC50 is > 236.9 mg/L for crustaceans (Daphnia magna), 72-hour ErC50 is > 978 mg/L for algae (Pseudokirchneriella subcapitata), and 96-hour LC50 is > 111.4 mg/L for fish (fathead minnow) (HSDB, 2005). Based on these values, the substance was classified into the "Not classified" category.
11 Hazardous to the aquatic environment （Long-term） Not classified - - - - Since it is water soluble (water solubility = 500 g/L (PHYSPROP Database, 2008)), and its classification for acute toxicity is "Not classified", the substance was classified into "Not classified".


NOTE:
* Classification was conducted by relevant Japanese Ministries in accordance with GHS Classification Guidance for the Japanese Government, and is intended to provide a reference for preparing GHS labelling and SDS for users.
* This is a provisional English translation of classification results and is subject to revision without notice.
* The responsibility for any resulting GHS labelling and SDS referenced from this site is with users.

Reference：
Reference Manual
Definitions / Abbreviations
Model Label by MHLW

 MHLW Website （in Japanese Only）
Model SDS by MHLW

 MHLW Website （in Japanese Only）

To GHS Information