GHS Classification Result

Chemical Name：Thiophene
CAS：110-02-1

Result：
ID: 21A3570
Classifier: Ministry of Health, Labour and Welfare （MHLW）, Ministry of the Environment （MOE）
Year Classified: FY2009
Reference Manual: GHS Classification Guidance by the Japanese Government （March, 2009）

PHYSICAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Explosives Not applicable - - - - There are no chemical groups associated with explosive properties present in the molecules.
2 Flammable gases （including chemically unstable gases） Not applicable - - - - Liquid (GHS definition)
3 Aerosols Not applicable - - - - Not aerosol products.
4 Oxidizing gases Not applicable - - - - Liquid (GHS definition)
5 Gases under pressure Not applicable - - - - Liquid (GHS definition)
6 Flammable liquids Category 2 Danger H225: Highly flammable liquid and vapour P303+P361+P353: IF ON SKIN （or hair）: Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.
P370+P378: In case of fire: Use ... for extinction.
P403+P235: Store in a well-ventilated place. Keep cool.
P210: Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P233: Keep container tightly closed.
P240: Ground/bond container and receiving equipment.
P241: Use explosion-proof electrical/ventilating/lighting/.../equipment.
P242: Use only non-sparking tools.
P243: Take precautionary measures against static discharge.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P501: Dispose of contents/container to ... 		Classified into Category 2 since flash point -9 degC (closed-cup) (GESTIS (access on Aug. 2009)) is <23 degC and initial boiling point 84.4 degC (Merck (14th, 2006)) is >35 degC. The substance is classified into Class 3, PG II in UNRTDG (UN2414).
7 Flammable solids Not applicable - - - - Liquid (GHS definition)
8 Self-reactive substances and mixtures Not applicable - - - - There are no chemical groups present in the molecule associated with explosive or self-reactive properties.
9 Pyrophoric liquids Not classified - - - - Auto-ignition point is 395 degC (Hommel (1996)) exceeding 70 degC.
10 Pyrophoric solids Not applicable - - - - Liquid (GHS definition)
11 Self-heating substances and mixtures Classification not possible - - - - Test methods applicable to liquid substances are not available.
12 Substances and mixtures which, in contact with water, emit flammable gases Not applicable - - - - The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).
13 Oxidizing liquids Not applicable - - - - Organic compounds containing no oxygen, fluorine or chlorine
14 Oxidizing solids Not applicable - - - - Liquid (GHS definition)
15 Organic peroxides Not applicable - - - - Organic compounds containing no bivalent -O-O- structure
16 Corrosive to metals Classification not possible - - - - No data available.

HEALTH HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Acute toxicity （Oral） Not classified - - - - Based on the result of no mortality (0/6) at dose level of 2000 mg/kg in rats test (OECD TG423) ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (access on Aug. 2009)), the substance was classified as "Not classified" in JIS Classification (correspond to Category 5 or "Not classified" in GHS classification).
1 Acute toxicity （Dermal） Classification not possible - - - - Classification not possible due to lack of data. As relevant information, a LD50 value is >20 mL/kg for guinea pigs (RTECS (2006)).
1 Acute toxicity （Inhalation: Gases） Not applicable - - - - Liquid (GHS definition)
1 Acute toxicity （Inhalation: Vapours） Classification not possible - - - - Classification not possible due to lack of data. As relevant information, there is information for a LC50 value for mice of 9500 mg/m3/2h (6.7 mg/L/4h = 1947 ppm/4h) (RTECS (2006)). Since the LC50 value (9.5 mg/L/2h) was lower than 90% of saturated vapour pressure concentration (361 mg/L), the test was considered to be conducted for vapour with almost no included mists.
1 Acute toxicity （Inhalation: Dusts and mists） Classification not possible - - - - No data available.
2 Skin corrosion/irritation Category 2 Warning H315: Causes skin irritation P302+P352: IF ON SKIN: Wash with plenty of soap and water.
P332+P313: If skin irritation occurs: Get medical advice/attention.
P264: Wash ... thoroughly after handling.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P321: Specific treatment （see ... on this label）.
P362: Take off contaminated clothing and wash before reuse. 		Based on a report that in man, tiophene has caused skin irritation (HSDB (2003)), the substance was classified into Category 2.
3 Serious eye damage/eye irritation Classification not possible - - - - No data available.
4 Respiratory sensitization Classification not possible - - - - No data available.
4 Skin sensitization Classification not possible - - - - No data available.
5 Germ cell mutagenicity Classification not possible - - - - Classification not possible due to lack of data from in vivo mutagenicity tests. From in vitro mutagenicity tests, there are reports of a negative reverse mutation test (Ames test) and a negative CHL cells chromosomal aberration test ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (access on Aug. 2009)).
6 Carcinogenicity Classification not possible - - - - No data available.
7 Reproductive toxicity Classification not possible - - - - In a combined repeat dose and reproductive/developmental toxicity screening test with rats (OECD TG422), as general toxicity, clinical signs such as decreased locomotor activity, prone position and salivation were observed at 100 mg/kg and/or 400 mg/kg, but no adverse effects on sexual function and fertility of parental animals or development of offspring ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (access on Aug. 2009)). However, poor maternal behavior and lactation were observed in the 100 mg/kg and more groups and the lower tendency of viability on postnatal day 4 in the 400 mg/kg group was attributed to the poor maternal behavior and lactation ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (access on Aug. 2009)). Since these dams showed degeneration of cerebellar nuclei neurons, poor maternal behavior and lactation were considered to be a secondary consequence by thiophene-treated ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (access on Aug. 2009)). In conclusion, classification was not possible since data for developmental toxicity including teratogenicity were insufficient.
8 Specific target organ toxicity - Single exposure Classification not possible - - - - In a rat oral test (OECD TG423), no death was observed. At 300 mg/kg, ptosis, salivation and soiled perineal region were noted, body weight gain was generally normal, and no gross pathological lesion was seen at the end of the observation period. At 2000 mg/kg, decreased locomotor activity, ptosis, salivation, staining around mouth and nose, and abnormal fur were noted. Body weight decreased on day 4 and tended to recover on day 8 of observation, but no gross pathological lesion was seen at necropsy ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (access on Aug. 2009)). Since no significant toxic effects were observed at 2000 mg/kg, the substance corresponds to "Not classified" with oral exposure. However, classification was not possible due to lack of data with other exposure routes.
9 Specific target organ toxicity - Repeated exposure Category 2 (liver, brain) Warning H373: May cause damage to organs through prolonged or repeated exposure (liver, brain) P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P314: Get medical advice/attention if you feel unwell.
P501: Dispose of contents/container to ... 		In a combined repeat dose and reproductive/developmental toxicity screening test with rats (OECD TG422) (for approximately 45 days), no death was observed in either sex but clinical signs such as irregular respiration, decreased locomotor activity, prone position and salivation were observed at 100 mg/kg in males and 400 mg/kg in females. On histopathological examination for males, hypertrophy of hepatocytes and infiltration of macrophages in the central zones were observed at 100 mg/kg and higher dosage levels. At 400 mg/kg, necrosis of hepatocytes and homogenous or vesicular cytoplasmic change of hepatocytes in the central zones were observed as well as increases in the concentrations of plasma total bilirubin and activities of various enzymes. Thus, hepatic toxicity was noted in the groups administered 100 mg/kg or higher doses. In addition, at 400 mg/kg slight toxicity to the cerebellum was indicated, since pyknosis/necrosis of granular cells in the cerebellum was observed for one male. In surviving females, hepatic changes similar to those observed in males of the 400 mg/kg group were found in the groups administered 100 mg/kg or higher doses. In addition, necrosis of cerebellar granular cells was observed in the groups given 100 mg/kg or more. In the 400 mg/kg group, dilatation and edema of the ventricles were evident. Thus, it was suggested that the substance at the dose level of 100 mg/kg or more is toxic to the liver and the cerebellum ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (access on Aug. 2009). Based on above information, the substance was classified into Category 2 (liver, brain).
10 Aspiration hazard Classification not possible - - - - No data available.

ENVIRONMENTAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
11 Hazardous to the aquatic environment （Acute） Category 3 - - H402: Harmful to aquatic life P273: Avoid release to the environment.
P501: Dispose of contents/container to ... 		Classified into Category 3 from its 48h-EC50 = 21 mg/L for Crustacea (Daphnia magna) (Test for the Ecological Effect of Chemical Substances (Ministry of the Environment), 1996).
11 Hazardous to the aquatic environment （Long-term） Category 3 - - H412: Harmful to aquatic life with long lasting effects P273: Avoid release to the environment.
P501: Dispose of contents/container to ... 		Classified into Category 3 since its acute toxicity is Category 3 and it is not rapidly degradable (Biodegradation and Bioconcentration of Existing Chemical Substances under the Chemical Substances Control Law, 1982).


NOTE:
* Classification was conducted by relevant Japanese Ministries in accordance with GHS Classification Guidance for the Japanese Government, and is intended to provide a reference for preparing GHS labelling and SDS for users.
* This is a provisional English translation of classification results and is subject to revision without notice.
* The responsibility for any resulting GHS labelling and SDS referenced from this site is with users.

Reference：
Reference Manual
Definitions / Abbreviations
Model Label by MHLW

 MHLW Website （in Japanese Only）
Model SDS by MHLW

 MHLW Website （in Japanese Only）

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