GHS Classification Result

Chemical Name：2-Butyne-1,4-diol
CAS：110-65-6

Result：
ID: 22A4026
Classifier: Ministry of Health, Labour and Welfare （MHLW）, Ministry of the Environment （MOE）
Year Classified: FY2010
Reference Manual: GHS Classification Guidance by the Japanese Government （July, 2010）

PHYSICAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Explosives Not classified - - - - Although including unsaturated C-C bonds, it is classified into Division 6.1, PGIII in UNRTDG (UN2716).
2 Flammable gases （including chemically unstable gases） Not applicable - - - - Solid (GHS definition)
3 Aerosols Not applicable - - - - Not aerosol products.
4 Oxidizing gases Not applicable - - - - Solid (GHS definition)
5 Gases under pressure Not applicable - - - - Solid (GHS definition)
6 Flammable liquids Not applicable - - - - Solid (GHS definition)
7 Flammable solids Classification not possible - - - - It is reported as flammable (ICSC (J) (2008)), but classification is not possible due to lack of additional data.
8 Self-reactive substances and mixtures Type G - - - - Although there are chemical groups (unsaturated C-C bonds) present in the molecule associated with self-reactive properties, the substance is classified into Division 6.1, PGIII in UNRTDG (UN2716), therefore it was judged that this substance is Type G.
9 Pyrophoric liquids Not applicable - - - - Solid (GHS definition)
10 Pyrophoric solids Not classified - - - - Since its auto ignition point is 335 degC (Ullmanns (E) (6th, 2003)), it is considered that the substance does not ignite at room temperature.
11 Self-heating substances and mixtures Classification not possible - - - - Test methods applicable to solid (melting point <= 140 degC) substances are not available.
12 Substances and mixtures which, in contact with water, emit flammable gases Not applicable - - - - The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).
13 Oxidizing liquids Not applicable - - - - Solid (GHS definition)
14 Oxidizing solids Not applicable - - - - The substance is an organic compound containing oxygen which is chemically bonded only to carbon or hydrogen.
15 Organic peroxides Not applicable - - - - Organic compounds containing no bivalent -O-O- structure.
16 Corrosive to metals Classification not possible - - - - Test methods applicable to solid substances are not available.

HEALTH HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Acute toxicity （Oral） Category 3 Danger H301: Toxic if swallowed P301+P310: IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P321: Specific treatment （see ... on this label）.
P330: Rinse mouth.
P405: Store locked up.
P501: Dispose of contents/container to ... 		Based on the rat LD50 values of 132 mg/kg bw (male) and 176 mg/kg bw (female) (EU RAR54 (2005)), the substance was classified into Category 3.
1 Acute toxicity （Dermal） Category 3 Danger H311: Toxic in contact with skin P302+P352: IF ON SKIN: Wash with plenty of soap and water.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P312: Call a POISON CENTER or doctor/physician if you feel unwell.
P322: Specific treatment （see ... on this label）.
P361: Remove/Take off immediately all contaminated clothing.
P363: Wash contaminated clothing before reuse.
P405: Store locked up.
P501: Dispose of contents/container to ... 		The three LD50 values for rats (424 mg/kg bw, 983 mg/kg bw and 1240 mg/kg bw) were documented (EU-RAR 54 (2005)). Since the two data are equivalent to the guidance value of Category 3 and the other is Category 4, the substance was classified as Category 3.
1 Acute toxicity （Inhalation: Gases） Not applicable - - - - Solid (GHS definition)
1 Acute toxicity （Inhalation: Vapours） Classification not possible - - - - No data available.
1 Acute toxicity （Inhalation: Dusts and mists） Category 3 Danger H331: Toxic if inhaled P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P403+P233: Store in a well-ventilated place. Keep container tightly closed.
P261: Avoid breathing dust/fume/gas/mist/vapours/spray.
P271: Use only outdoors or in a well-ventilated area.
P311: Call a POISON CENTER or doctor/physician.
P321: Specific treatment （see ... on this label）.
P405: Store locked up. 		Based on the rat LC50 value of 0.69 mg/L/4h (EU-RAR 54 (2005)), the substance was classified into Category 3. According to the description of liquid aerosol for the test substance, criterion value for dust/mist was adopted.
2 Skin corrosion/irritation Category 1 Danger H314: Causes severe skin burns and eye damage P301+P330+P331: IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P303+P361+P353: IF ON SKIN （or hair）: Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.
P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P264: Wash ... thoroughly after handling.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P310: Immediately call a POISON CENTER or doctor/physician.
P321: Specific treatment （see ... on this label）.
P363: Wash contaminated clothing before reuse.
P405: Store locked up.
P501: Dispose of contents/container to ... 		In the rabbit test (OECD TG 404) treated 6 animals with 0.5 g for 4 hours, severe erythema and severe edema within 24 hours, and necrosis within 6 days were noted in all animals. Based on the information, the substance was classified as Category 1. The substance was classified as "R34" in the EU classification (EU-Annex I (Access on May 2010))
3 Serious eye damage/eye irritation Category 1 Danger H318: Causes serious eye damage P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P310: Immediately call a POISON CENTER or doctor/physician. 		In the rabbit tests (OECD TG 405), instillation of 100 mg of test substance caused moderate irritation of iris, cornea and conjunctivae in all 6 rabbits, and 1/6 rabbits exhibited irreversible corneal opacity (EU-RAR 54 (2005)). Based on these information, the substance was classified into Category 1. As for this substance, skin corrosion was also reported (EU RAR 54 (2005)).
4 Respiratory sensitization Classification not possible - - - - No data available.
4 Skin sensitization Category 1 Warning H317: May cause an allergic skin reaction P302+P352: IF ON SKIN: Wash with plenty of soap and water.
P333+P313: If skin irritation or rash occurs: Get medical advice/attention.
P261: Avoid breathing dust/fume/gas/mist/vapours/spray.
P272: Contaminated work clothing should not be allowed out of the workplace.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P321: Specific treatment （see ... on this label）.
P363: Wash contaminated clothing before reuse.
P501: Dispose of contents/container to ... 		Dermatitis developed by the occupational exposure and positive reaction of patch tests were reported. There were many documents that the substance is allergenic (EU-RAR 54 (2005)). The substance was classified as "R43" in the EU classification (EU-Annex I (Access on May 2010)). Based on these documents, the substance was classified as Category 1.
5 Germ cell mutagenicity Not classified - - - - The substance was classified as "Not classified" based on the negative results in the micronucleus test using bone marrow cells by intraperitoneal administration to mice (in vivo mutagenicity test in somatic cells) (EU-RAR 54 (2005)). As relevant information, as for in vitro studies, negative results in the Ames test (NTP DB (Access on May 2010)) and equivocal results in the chromosomal aberration test using V79 cells (EU-RAR 54 (2005)) were reported.
6 Carcinogenicity Classification not possible - - - - No data available.
7 Reproductive toxicity Not classified - - - - In the one-generation reproductive study (OECD TG415) in which male and female rats were administered via drinking water during the period of premating, mating, gestation and lactation, signs of developmental retardation (impaired postnatal weight gain associated with a delay in sexual maturation) were observed in the F1 progeny at the dose level causing general toxicity to parent animals. However, there was no adverse effect on reproductive ability and no indication of adverse impairment in reproductive organs for both sexes, spermatic indices for males and estrous cycle for females (EU-RAR 54 (2005)). In the developmental toxicity study (OECD TG 414) using pregnant rat administered orally during the organogenetic period, no substance-related impairment was observed in gestational parameters, fetal viability, gross morphological appearance. Embriotoxic, fetotoxic or teratogenic potential was not identified (EU-RAR 54 (2005)). As mentioned above, in the study that conforms to the OECD guidelines, there were no adverse effects on sexual functions and fertility of parental animals, and development of offspring. Therefore, the substance was classified as "Not classified".
8 Specific target organ toxicity - Single exposure Category 1 (systemic toxicity) Danger H370: Causes damage to organs (systemic toxicity) P307+P311: IF exposed: Call a POISON CENTER or doctor/physician.
P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P321: Specific treatment （see ... on this label）.
P405: Store locked up.
P501: Dispose of contents/container to ... 		It was reported that the main clinical signs of toxicity appeared after oral treatment in rats were apathy, dysequilibrium, convulsions, tremors, etc. (EU-RAR 54 (2005)), while there was no concern of the substance affecting on the nervous system according to all data obtained at present (EU-RAR 54 (2005)). In rats applied dermally up to 5000 mg/kg, the changes in livers and kidneys demonstrated severe hyperemia and degeneration containing necrosis (EU-RAR 54 (2005)), which were the findings observed at higher doses beyond the upper limit of the guidance values. Otherwise, respiratory effects were also noted in both inhalation and dermal exposure (EU-RAR 54 (2005)). As stated above, it was difficult to identify the target organ(s) and the values of LD50 or LC50 were ranged within Category 1 of the guidance values. Thus, the substance was classified as Category 1 (systemic toxicity).
9 Specific target organ toxicity - Repeated exposure Category 1 (hematopoietic system, liver), Category 2 (kidney) Danger
Warning 		H372: Cause damage to organs through prolonged or repeated exposure (hematopoietic system, liver)
H373: May cause damage to organs through prolonged or repeated exposure (kidney) 		P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P314: Get medical advice/attention if you feel unwell.
P501: Dispose of contents/container to ... 		In the 28-day oral study in rats, significantly decreased corpuscular indices suggesting anemia, mononuclear cell infiltration and numerous megakaryocytes in the red pulp of the spleen were observed at 10 mg/kg/day (converted dose level as that of 90-day study: 3.1 mg/kg/day (equivalent to Category 1)) or more (EU-RAR 54 (2005)). In addition, microscopic examination revealed the liver lesions at 10 mg/kg/day (converted dose level as that of 90-day study: 3.1 mg/kg/day (eq. Category 1)) or more, which consisted of hypertrophy of parenchymal cells, increased polymorphonuclear hepatocytes, some parenchymal cells with macronucleus and chromatin margination, etc. (EU-RAR 54 (2005)). Furthermore, in histopathology of the animals that died at 50 mg/kg/day (converted dose level as that of 90-day study: 15.6 mg/kg/day (eq. Category 2)), renal tubular degeneration and interstitial mononuclear cell infiltration in the kidney were noted (EU-RAR 54 (2005)). As described above, the main toxicity appeared in hematopoietic systems, liver, and kidneys, and with reference to the guidance values from the doses appeared, the substance was classified as Category 1 (hematopoietic systems, liver) and as Category 2 (kidney).
10 Aspiration hazard Classification not possible - - - - No data available.

ENVIRONMENTAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
11 Hazardous to the aquatic environment （Acute） Category 3 - - H402: Harmful to aquatic life P273: Avoid release to the environment.
P501: Dispose of contents/container to ... 		Classified into Category 3 from its 48h-EC50 = 26.8 mg/L for crustacea (Daphnia magna) (EU-RAR, 2005).
11 Hazardous to the aquatic environment （Long-term） Not classified - - - - Classified as "Not classified" since it is rapidly degradable (DOC degradation rate tested by OECD 301: 100% (EU-RAR, 2005)) and its bioaccumulation potential is estimated to be low (Log Kow = -0.93 (PHYSPROP Database, 2009)) though its acute toxicity is Category 3.
12 Hazardous to the ozone layer Classification not possible - - - - This substance is not listed in Annexes to the Montreal Protocol.


NOTE:
* Classification was conducted by relevant Japanese Ministries in accordance with GHS Classification Guidance for the Japanese Government, and is intended to provide a reference for preparing GHS labelling and SDS for users.
* This is a provisional English translation of classification results and is subject to revision without notice.
* The responsibility for any resulting GHS labelling and SDS referenced from this site is with users.

Reference：
Reference Manual
Definitions / Abbreviations
Model Label by MHLW

 MHLW Website （in Japanese Only）
Model SDS by MHLW

 MHLW Website （in Japanese Only）

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