GHS Classification Result

Chemical Name：3,4-Dichloro-1-butene
CAS：760-23-6

Result：
ID: 22A4048
Classifier: Ministry of Health, Labour and Welfare （MHLW）, Ministry of the Environment （MOE）
Year Classified: FY2010
Reference Manual: GHS Classification Guidance by the Japanese Government （July, 2010）

PHYSICAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Explosives Not applicable - - - - There are no chemical groups associated with explosive properties present in the molecule.
2 Flammable gases （including chemically unstable gases） Not applicable - - - - Liquid (GHS definition)
3 Aerosols Not applicable - - - - Not aerosol products.
4 Oxidizing gases Not applicable - - - - Liquid (GHS definition)
5 Gases under pressure Not applicable - - - - Liquid (GHS definition)
6 Flammable liquids Category 3 Warning H226: Flammable liquid and vapour P303+P361+P353: IF ON SKIN （or hair）: Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.
P370+P378: In case of fire: Use ... for extinction.
P403+P235: Store in a well-ventilated place. Keep cool.
P210: Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P233: Keep container tightly closed.
P240: Ground/bond container and receiving equipment.
P241: Use explosion-proof electrical/ventilating/lighting/.../equipment.
P242: Use only non-sparking tools.
P243: Take precautionary measures against static discharge.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P501: Dispose of contents/container to ... 		Since its flash point reported 33 degC (closed-cup) (IUCLID (2000)) is >= 23 degC and <= 60 degC, the substance corresponds to Category 3.
7 Flammable solids Not applicable - - - - Liquid (GHS definition)
8 Self-reactive substances and mixtures Classification not possible - - - - Although there are chemical groups (an unsaturated bond) present in the molecule associated with self-reactive properties, classification is not possible due to lack of additional data.
9 Pyrophoric liquids Not classified - - - - There is a description, "Flammability: 2 (This degree includes materials that must be moderately heated before ignition will occur)" (HSDB (2003)). It is considered that the substance is not pyrophoric at room temperature.
10 Pyrophoric solids Not applicable - - - - Liquid (GHS definition)
11 Self-heating substances and mixtures Classification not possible - - - - Test methods applicable to liquid substances are not available.
12 Substances and mixtures which, in contact with water, emit flammable gases Not applicable - - - - The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).
13 Oxidizing liquids Not applicable - - - - The substance is an organic compound containing chlorine which is chemically bonded only to carbon.
14 Oxidizing solids Not applicable - - - - Liquid (GHS definition)
15 Organic peroxides Not applicable - - - - Organic compounds containing no bivalent -O-O- structure.
16 Corrosive to metals Classification not possible - - - - No data available.

HEALTH HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Acute toxicity （Oral） Category 4 Warning H302: Harmful if swallowed P301+P312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P330: Rinse mouth.
P501: Dispose of contents/container to ... 		The rat LD50 values of 943 mg/kg (male), 946 mg/kg (female) ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council (1996)), 879 mg/kg (SIDS (2001)) and 577 - 1153 mg/kg (SIDS (2001)) were documented. These all data are corresponded to Category 4.
1 Acute toxicity （Dermal） Not classified - - - - The rabbit LD50 value of > 2000 mg/kg and no mortality was reported (SIDS (2001)). The substance was classified as "Not Classified".
1 Acute toxicity （Inhalation: Gases） Not applicable - - - - Liquid (GHS definition)
1 Acute toxicity （Inhalation: Vapours） Category 3 Danger H331: Toxic if inhaled P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P403+P233: Store in a well-ventilated place. Keep container tightly closed.
P261: Avoid breathing dust/fume/gas/mist/vapours/spray.
P271: Use only outdoors or in a well-ventilated area.
P311: Call a POISON CENTER or doctor/physician.
P321: Specific treatment （see ... on this label）.
P405: Store locked up. 		Based on the rat LC50 value of 2100 ppm/4hrs (SIDS (2001)), the substance was classified as Category 3. Since the LC50 value was lower than 90% of the saturated vapor pressure concentration (28816 ppm), the criterion values for gas were adopted for the classification.
1 Acute toxicity （Inhalation: Dusts and mists） Classification not possible - - - - No data available.
2 Skin corrosion/irritation Not classified - - - - In the 4-hour rabbit test (OECD TG404, GLP-compliant), application of 0.5 ml of the undiluted test substance for three animals caused slightly irritating with erythema (grade 1 or 2) until 72 hours after removal of patch in all animals. And the mean scores of erythema or edema at 24, 48 and 72 hours after removal of the patch were less than 2.3 (SIDS (2001)). Based on the document, the substance was classified as "Not classified" in the JIS classification (corresponding to Category 3 in the UN-GHS classification).
3 Serious eye damage/eye irritation Not classified - - - - In the rabbit test (OECD TG 405, GLP-compliant), instillation of 0.1ml of the undiluted test substance to conjunctival sac for three animals caused corneal opacity (grade 1) and iritis (grade 1) in 1 animal at 1-48 hours after instillation, and conjunctival redness (grade 1) in all three animals at 1 hour after instillation. Therefore, this substance was judged to be slight irritant. The mean scores of corneal opacity and iritis after 24, 48 and 72 hours of instillation was both 0 in 2 of 3 animals and those of conjunctival redness and edema were both under 2 in all 3 animals (SIDS (2001)). Based on the document, the substance was classified as "Not classified".
4 Respiratory sensitization Classification not possible - - - - No data available.
4 Skin sensitization Classification not possible - - - - In the guinea pig test, the substance was reported as not sensitizing (SIDS (2001)). However, because the details containing test methods were unknown, the substance was classified as "Classification not possible".
5 Germ cell mutagenicity Category 2 Warning H341: Suspected of causing genetic defects P308+P313: IF exposed or concerned: Get medical advice/attention.
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P281: Use personal protective equipment as required.
P405: Store locked up.
P501: Dispose of contents/container to ... 		The classification was concluded as Category 2 based on the positive results in the chromosomal aberration test using bone marrow cells obtained from the rats that were exposed by inhalation (in vivo mutagenicity test in somatic cells) (SIDS (2001)). As relevant information, as for in vitro mutagenicity studies, positive results in the Ames test, the chromosome aberration test using CHL/IU cells ("Toxicity Testing Reports of Environmental Chemicals"(Chemicals Investigation Promoting Council) (1996)) and the HGPRT gene mutation assay using CHO cells (SIDS (2001)) were reported.
6 Carcinogenicity Classification not possible - - - - No data available.
7 Reproductive toxicity Classification not possible - - - - In the combined repeated oral dose toxicity study with the reproduction/developmental toxicity screening test in rats (OECD TG 422, GLP-compliant), no adverse effects were documented on sexual functions and fertility containing the copulation index, fertility index, gestation length, gestation index and state of lactation in maternal animals. No treatment-related effect on the number of offspring and live pups, sex ratio, live birth index, viability index and morphological findings were detected in any of the offspring ("Toxicity Testing Reports of Environmental Chemicals"(Chemicals Investigation Promoting Council) (1996)). However, since the inspection for the fetal developmental at the end stage of gestation period was not performed, data were insufficient. Therefore, the substance was classified as "Classification not possible".
8 Specific target organ toxicity - Single exposure Category 1 (liver), Category 3 (narcotic effects) Danger
Warning 		H370: Causes damage to organs (liver)
H336: May cause drowsiness or dizziness (narcotic effects) 		P307+P311: IF exposed: Call a POISON CENTER or doctor/physician.
P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P321: Specific treatment （see ... on this label）.
P405: Store locked up.
P501: Dispose of contents/container to ...
P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P403+P233: Store in a well-ventilated place. Keep container tightly closed.
P261: Avoid breathing dust/fume/gas/mist/vapours/spray.
P271: Use only outdoors or in a well-ventilated area.
P312: Call a POISON CENTER or doctor/physician if you feel unwell. 		In the single inhalation study in rats exposed to vaporized test substance to 3000 ppm/4hrs (15.3 mg/L/4hrs), dead animals the day of exposure showed centrilobular reduction of hepatocellular glycogen and fatty change. Even in the rats exposed to 1000 ppm/4hrs (5.11 mg/L/4hrs), equivalent to Category 1 of the guidance values, progress of liver lesions that led to centrilobular necrosis were observed after one day of exposure ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (1996)). Based on these findings, the substance was classified as Category 1(liver). Moreover, in the acute oral toxicity study in rats (LD50 value: 943-946 mg/kg), clinical signs observed after administration included decreased locomotor activity, deep respiration, ptosis, salivation, muscular relaxation, prone position, piloerection, etc. (SIDS (2001)). In another acute oral toxicity study in rats (LD50 value: 577-1153 mg/kg), the signs of weakness and narcotic symptoms were reported (SIDS (2001)). Further, clinical signs containing indifference to auditory and tactile stimuli in the acute dermal study (LD50 value: > 2000 mg/kg), as well as incoordination and refractory in the acute (vapor) inhalation study (LC50 value: 2100 ppm/4hrs (10.7 mg/L/4hrs)) were documented (SIDS (2001)). Based on the information, the substance was classified as Category 3 (narcotic effects).
9 Specific target organ toxicity - Repeated exposure Category 1 (liver) Danger H372: Cause damage to organs through prolonged or repeated exposure (liver) P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P314: Get medical advice/attention if you feel unwell.
P501: Dispose of contents/container to ... 		In the combined repeated oral dose toxicity study with the reproduction/developmental toxicity screening test in rats ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (1996)), increase in the liver weight and hypertrophy of hepatocytes were observed at 50 mg/kg/day (converted dose level as that of 90-day study: approx. 23 mg/kg/day). In the 6-month oral toxicity study in rats, degenerative changes in the liver were also reported (SIDS (2001)). Furthermore, it was described that exposure of the vapor of the substance to rats for 2 weeks at 1.037 mg/L/6hrs (converted value as that of 90-day study: 0.161 mg/L/6hrs) revealed increased relative liver weight and morphological changes in liver, and essential toxicity was hepatocellular hypertrophy (SIDS (2001)). Based on the findings described above, since the effect concentration in inhalation study was equivalent to Category 1 within the guidance values, the substance was classified as Category 1 (liver).
10 Aspiration hazard Classification not possible - - - - No data available.

ENVIRONMENTAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
11 Hazardous to the aquatic environment （Acute） Category 2 - - H401: Toxic to aquatic life P273: Avoid release to the environment.
P501: Dispose of contents/container to ... 		Classified into Category 2 from its 48h-EC50 = 10 mg/L for crustacea (Daphnia magna) (Test for the Ecological Effect of Chemical Substances (Ministry of the Environment), 1996).
11 Hazardous to the aquatic environment （Long-term） Category 2 - H411: Toxic to aquatic life with long lasting effects P273: Avoid release to the environment.
P391: Collect spillage.
P501: Dispose of contents/container to ... 		Classified into Category 2 since its acute toxicity is Category 2 and it is not rapidly degradable (28d-BOD degradation rate = 1 - 28% (Biodegradation and Bioconcentration of Existing Chemical Substances under the Chemical Substances Control Law, 1979)).
12 Hazardous to the ozone layer Classification not possible - - - - This substance is not listed in Annexes to the Montreal Protocol.


NOTE:
* Classification was conducted by relevant Japanese Ministries in accordance with GHS Classification Guidance for the Japanese Government, and is intended to provide a reference for preparing GHS labelling and SDS for users.
* This is a provisional English translation of classification results and is subject to revision without notice.
* The responsibility for any resulting GHS labelling and SDS referenced from this site is with users.

Reference：
Reference Manual
Definitions / Abbreviations
Model Label by MHLW

 MHLW Website （in Japanese Only）
Model SDS by MHLW

 MHLW Website （in Japanese Only）

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