GHS Classification Result (Revision of the past classification result)

Chemical Name:Atrazine
CAS:1912-24-9

Result:
ID: 22B4507
Classifier: Ministry of Health, Labour and Welfare (MHLW), Ministry of the Environment (MOE)
Year Classified: FY2010
Reference Manual: GHS Classification Guidance by the Japanese Government (July, 2010)

PHYSICAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Explosives Not applicable - - - - There are no chemical groups associated with explosive properties present in the molecule.
2 Flammable gases (including chemically unstable gases) Not applicable - - - - Solid (GHS definition)
3 Aerosols Not applicable - - - - Not aerosol products.
4 Oxidizing gases Not applicable - - - - Solid (GHS definition)
5 Gases under pressure Not applicable - - - - Solid (GHS definition)
6 Flammable liquids Not applicable - - - - Solid (GHS definition)
7 Flammable solids Not classified - - - - Based on the information of nonflammable (IUCLID (2000) test method: Directive 84/449/EEC A10), the substance is classified as "Not classified".
8 Self-reactive substances and mixtures Not applicable - - - - There are no chemical groups present in the molecule associated with explosive or self-reactive properties.
9 Pyrophoric liquids Not applicable - - - - Solid (GHS definition)
10 Pyrophoric solids Classification not possible - - - - Insufficient data available. It is reported as "No self ignition" (IUCLID (2000)) measured by the method of Directive 84/449/EEC A16, but no basis of pyrophoric properties in GHS is derived from this data. Therefore it is classified as "Classification not possible".
11 Self-heating substances and mixtures Classification not possible - - - - Insufficient data available. It is reported as non flammable measured by the method of Directive 84/449/EEC A 10 (IUCLID (2000)), but no basis of self-heating properties in GHS is derived from this data. Therefore it is classified as "Classification not possible".
12 Substances and mixtures which, in contact with water, emit flammable gases Not applicable - - - - The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).
13 Oxidizing liquids Not applicable - - - - Solid (GHS definition)
14 Oxidizing solids Not applicable - - - - The substance is an organic compound containing chlorine which is chemically bonded only to carbon.
15 Organic peroxides Not applicable - - - - Organic compounds containing no bivalent -O-O- structure
16 Corrosive to metals Classification not possible - - - - Test methods applicable to solid substances are not available. As relevant note, it is reported as "noncorrosive to equipment and metal surfaces" (HSDB (2004)).

HEALTH HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Acute toxicity (Oral) Category 4 Warning H302: Harmful if swallowed P301+P312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P330: Rinse mouth.
P501: Dispose of contents/container to ...
Eight LD50 values of 3250, 3000 mg/kg (both from Registration and application document of pesticide (2002)), 2000, 670, 740, 2300 mg/kg (all from IARC 73 (1999)), 1471 and 1212 mg/kg (both from ATSDR (2003)) for rats were reported. The five of them corresponded to Category 4, and the other three corresponded to "Not classified" in the JIS classification. The substance was classified as Category 4, as most of the data corresponded to it.
1 Acute toxicity (Dermal) Not classified - - - - Three LD50 values of > 5000, > 2000 mg/kg (both from Registration and application document of pesticide (2002)) and > 3100 mg/kg (PATTY, 5th (2001))) for rats and the one LD50 value of 9300 mg/kg (PATTY, 5th (2001) for rabbits were reported. All of them correspond to "Not classified" in the JIS classification (corresponding to Category 5 or "Not classified" in the UN-GHS classification).
1 Acute toxicity (Inhalation: Gases) Not applicable - - - - Solid (GHS definition)
1 Acute toxicity (Inhalation: Vapours) Classification not possible - - - - No data available.
1 Acute toxicity (Inhalation: Dusts and mists) Not classified - - - - Based on the rat LC50 values of > 5.148 mg/L/4hrs and > 5.82 mg/L/4hrs (Registration and application document of pesticide (2002)), > 2.84 mg/L/4hrs and 8 mg/L/4hrs (both from PATTY, 5th (2001)), the substance was classified as "Not classified" in the JIS classification (corresponding to Category 5 or "Not classified" in the UN-GHS classification). The test concentration was higher than the saturated vapor pressure concentration (3.35E-06 mg/L), the criterion values for dust/mist were adopted.
2 Skin corrosion/irritation Not classified - - - - In the rabbit test of technical product of pesticide, 24-hour application of 0.5 g of the substance caused slight irritation (Registration and application document of pesticide (1989)). In another rabbit test, 24-hour application of 0.5 g of the substance caused no irritation (IUCLID (2000)). The substance was minimally irritating to rabbit skin (PATTY, 5th (2001)). Based on these documents, the substance was classified as "Not classified" in the JIS classification (corresponding to Category 3 or "Not classified" in the UN-GHS classification).
3 Serious eye damage/eye irritation Category 2B - Warning H320: Causes eye irritation P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P337+P313: If eye irritation persists: Get medical advice/attention.
P264: Wash ... thoroughly after handling.
In the rabbit test, instillation of 0.1 g of the technical product caused no irritation, and caused slight irritation with 50% water dispersible formulation (wettable powder) of the substance (Registration and application document of pesticide (1989)). And it was reported that the substance caused mild irritation to rabbit eye (PATTY, 5th (2001)). Based on these documents, the substance was classified as Category 2B.
4 Respiratory sensitization Classification not possible - - - - No data available.
4 Skin sensitization Category 1 Warning H317: May cause an allergic skin reaction P302+P352: IF ON SKIN: Wash with plenty of soap and water.
P333+P313: If skin irritation or rash occurs: Get medical advice/attention.
P261: Avoid breathing dust/fume/gas/mist/vapours/spray.
P272: Contaminated work clothing should not be allowed out of the workplace.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P321: Specific treatment (see ... on this label).
P363: Wash contaminated clothing before reuse.
P501: Dispose of contents/container to ...
In the guinea pig maximization test (OECD TG 406) of technical product of pesticide, skin reaction was observed in 17 of 20 animals, and positive rate was 85% (Registration and application document of pesticide (1997)). In other guinea pig maximization test (OECD TG 406, GLP-compliant), 65 - 70% of animals revealed skin reaction (positive rate), and it was reported that the substance was classified as a sensitizer (IUCLID (2000)). Based on these documents, the substance was classified as Category 1. As relevant information, 50% water dispersible formulation (wettable powder) of the substance did not cause sensitization in the Buehler test (Registration and application document of pesticide (1997)). And in the repeated patch test that 50 human volunteers were dermally exposed, no signs of dermal reaction were observed and resulted as "not sensitizing" (IUCLID (2000)).
5 Germ cell mutagenicity Classification not possible - - - - The dominant lethal test using mice orally administered (in vivo inheritable mutagenicity test in germ cells) resulted weakly positive (ATSDR (2003)). However, since the test was conducted only at one dose level, therefore, the evaluation was impossible. As for the in vivo mutagenicity test in somatic cells, in the micronucleus test using bone marrow cell from mice orally administered, positive results in females and negative in males, showing a contradictory result, were noted (ATSDR (2003)). However, there was no description about the sex difference, and only one animal was used in the negative control group, therefore, the evaluation was impossible. In the chromosome aberration test using bone marrow cell obtained from mice orally administered via drinking water for 90 days at the dose level of 20 ppm, negative results were reported (ATSDR (2003)). However, it was difficult to evaluate the validity of the dose level of 20 ppm and the effects by the short-term high concentration exposure. Although another multiple in vivo test data, such as negative result in the micronucleus test using bone marrow from mice intraperitoneally administered, and negative results in the stomach, liver and kidney, but positive in the lung in the DNA damage test (in vivo genotoxicity test in somatic cells) using rats orally administered (ATSDR (2003)), were obtained, both of them were unable to be evaluated for classification. Therefore, the substance was classified as "Classification not possible". As relevant information, as for in vitro studies, negative results were reported in the Ames test (NTP DB (Access on Jun. 2009)) and chromosome aberration test using human lymphocytes and CHO cells (IARC 73 (1999)).
6 Carcinogenicity Classification not possible - - - - The substance was classified as "Classification not possible" based on the criteria of "Group 3" in the carcinogenicity assessment of the IARC (IARC 73 (1999)) and "A4" in the assessment of the ACGIH (ACGIH (2001)). As relevant information, no increase in the treatment-related incidence of tumors were reported in the 91-week dietary administration test in mice (IARC 73 (1999)). In the 104-week dietary administration test in rats, significant effects were not indicated in any dose groups (IARC 73 (1999)). In the maximum 106-week dietary administration test in SD rats, significantly increase in the incidences of fibroadenoma and adenocarcinoma of the mammary gland in females were reported (IARC 73 (1999)).
7 Reproductive toxicity Category 2 Warning H361: Suspected of damaging fertility or the unborn child P308+P313: IF exposed or concerned: Get medical advice/attention.
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P281: Use personal protective equipment as required.
P405: Store locked up.
P501: Dispose of contents/container to ...
In the two generation study in rats that administrated by feeding from 10 weeks before mating, at the maximum tolerated dose (500 ppm), any adverse effects on the reproductive performance or on the prenatal and postnatal parameters were not caused, no treatment-related malformations, embryotoxicity or specific fetotoxicity were also observed (IUCLID (2000)). In the oral administration test during the organogenetic period in rabbits, abortions in 2/19 dams, increased incidence of fetal resorptions and post-implantation loss were observed at the dose (75 mg/kg) that reduced feed consumption and reduced body weight was observed in dams (IARC 73 (1999)). Therefore, the substance was classified as Category 2. In addition, in the administration test during the organogenetic period in rabbits, there were no dose-related increases in the incidence of malformations.
8 Specific target organ toxicity - Single exposure Category 2 (nervous system) Warning H371: May cause damage to organs (nervous system) P309+P311: IF exposed or if you feel unwell: Call a POISON CENTER or doctor/physician.
P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P405: Store locked up.
P501: Dispose of contents/container to ...
In multiple acute oral toxicity studies, sedation, dyspnea, exophthalmos etc. were reported within the dose range of 600 - 6000 mg/kg, and hypoactivity, ataxia, salivation, etc. were reported in another study within the dose range of 2000 - 5500 mg/kg for rats. For mice, hypoactivity, ptosis, ataxia, tremor etc. were reported within the dose range of 444 - 1332 mg/kg, and sedation, dyspnea etc. were reported in another study within the dose range of 1670 - 6000 mg/kg (IUCLID (2000)). While, in the study about neurological effects after single oral administration of 100 mg/kg to rats, the effects on the cerebellum containing reduction in cerebellar activity and abolish of the evoked spiked activity of Purkinje cells following stimulation of the radial nerves etc. were reported (ATSDR (2000)). As described above, the effects on the nervous system were suggested at the dose range equivalent to Category 2 of the guidance values or higher, judging from the signs observed in the acute oral toxicity studies. In consideration with the study report indicating the effects on the cerebellum as well, the substance was classified as Category 2 (nervous system). Otherwise, no serious toxic effects were found either at the concentration of 5.1 mg/L (as dusts) in inhalation route or at 2000 mg/kg in dermal route (IUCLID (2000)).
9 Specific target organ toxicity - Repeated exposure Category 2 (heart, liver, kidney) Warning H373: May cause damage to organs through prolonged or repeated exposure (heart, liver, kidney) P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P314: Get medical advice/attention if you feel unwell.
P501: Dispose of contents/container to ...
In the 7-day or 14-day oral administration study in rats treated with 100 - 600 mg/kg/day (converted dose levels as that of 90-day study: 15.6 - 93.3 mg/kg/day), it was reported that hepatotoxicity such as increase of serum GPT and alkaline phosphatase activities, and degeneration of the smooth endoplasmic reticulum, lipid drop accumulation and swollen mitochondria as electron micrographic findings were observed in all treatment groups except for the lowest dose group, and that renal toxicity containing proteinuria, reduced creatinine clearance and increased urinary electrolyte output were observed in all treatment groups (IARC 73 (1999)). Since the test dose levels ranged within Category 2 of the guidance values, the substance was classified as Category 2 (liver, kidney). While in the one-year feeding study in dogs, the most significant effect was the cardiopathy which was characterized by discrete myocardial degeneration, which was the most prominently found in animals receiving 1000 ppm (ca. 34 mg/kg/day). In the study, cardiac toxicity such as ascites, cachexia, labored/shallow breathing, and abnormal changes on the electrocardiograph (ECG) were reported, and dilatation of the right atrium as gross pathological examination, microscopically atrophy and myelosis were reported (IRIS (2004)). Based on the information, the substance was classified as Category 2 (heart).
10 Aspiration hazard Classification not possible - - - - No data available.

ENVIRONMENTAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
11 Hazardous to the aquatic environment (Acute) Category 1 Warning H400: Very toxic to aquatic life P273: Avoid release to the environment.
P391: Collect spillage.
P501: Dispose of contents/container to ...
Classified into Category 1 from its 96h-EbC50 = 0.147 mg/L for algae (Skeletonema costatum) (ECETOC TR91, 2003).
11 Hazardous to the aquatic environment (Long-term) Category 1 Warning H410: Very toxic to aquatic life with long lasting effects P273: Avoid release to the environment.
P391: Collect spillage.
P501: Dispose of contents/container to ...
Classified into Category 1 since its acute toxicity is Category 1 and it is not rapidly degradable (BOD degradation rate: 1% (Biodegradation and Bioconcentration of Existing Chemical Substances under the Chemical Substances Control Law, 2002)).
12 Hazardous to the ozone layer Classification not possible - - - - This substance is not listed in Annexes to the Montreal Protocol.


NOTE:
* Classification was conducted by relevant Japanese Ministries in accordance with GHS Classification Guidance for the Japanese Government, and is intended to provide a reference for preparing GHS labelling and SDS for users.
* This is a provisional English translation of classification results and is subject to revision without notice.
* The responsibility for any resulting GHS labelling and SDS referenced from this site is with users.

Reference:
Reference Manual

Definitions / Abbreviations

Model Label by MHLW

MHLW Website (in Japanese Only)

Model SDS by MHLW

MHLW Website (in Japanese Only)


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