NITE - Chemical Management Field

Latest GHS Classification Results by the Japanese Government (edited by NITE)

GENERAL INFORMATION

Item	Information
CAS RN	95-50-1
Chemical Name	o-Dichlorobenzene
Substance ID	m-nite-95-50-1_v1
Download of Excel format	Excel file

REFERENCE INFORMATION

Item	Information
Guidance used for the classification (External link)	To Guidance List
UN GHS document (External link)	To UN GHS document
FAQ（GHS classification results by the Japanese Government）	To FAQ
List of Information Sources (Excel file)	List of Information Sources
List of Definitions/Abbreviations	Definitions/Abbreviations
Sample Label by MHLW (External link)	To Workplace Safety Site (MHLW)
Sample SDS by MHLW (External link)	To Workplace Safety Site (MHLW)
OECD/eChemPortal (External link)	To OECD/eChemPortal (External link)

PHYSICAL HAZARDS

Hazard class		Classification	Pictogram Signal word	Hazard statement (code)	Precautionary statement (code)	Rationale for the classification	Classification year (FY)	GHS Classification Guidance for the Japanese Government
1	Explosives	Not classified (Not applicable)	- -	-	-	There are no chemical groups associated with explosive properties present in the molecule.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
2	Flammable gases	Not classified (Not applicable)	- -	-	-	Liquid (GHS definition)	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
3	Aerosols	Not classified (Not applicable)	- -	-	-	Not aerosol products.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
4	Oxidizing gases	Not classified (Not applicable)	- -	-	-	Liquid (GHS definition)	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
5	Gases under pressure	Not classified (Not applicable)	- -	-	-	Liquid (GHS definition)	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
6	Flammable liquids	Category 4	- Warning	H227	P370+P378 P210 P280 P403 P501	Based on a flash point of 66 deg C (closed cup) (ICSC (2003)), it was classified in Category 4.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
7	Flammable solids	Not classified (Not applicable)	- -	-	-	Liquid (GHS definition)	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
8	Self-reactive substances and mixtures	Not classified (Not applicable)	- -	-	-	There are no chemical groups present in the molecule associated with explosive or self-reactive properties.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
9	Pyrophoric liquids	Not classified	- -	-	-	It is estimated that it does not ignite at normal temperatures from an autoignition temperature of 648 deg C (HSDB (Access on August 2015)).	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
10	Pyrophoric solids	Not classified (Not applicable)	- -	-	-	Liquid (GHS definition)	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
11	Self-heating substances and mixtures	Classification not possible	- -	-	-	Test methods applicable to liquid substances are not available.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
12	Substances and mixtures which, in contact with water, emit flammable gases	Not classified (Not applicable)	- -	-	-	The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
13	Oxidizing liquids	Not classified (Not applicable)	- -	-	-	The substance is an organic compound containing chlorine (but not fluorine or oxygen) which is chemically bonded only to carbon or hydrogen.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
14	Oxidizing solids	Not classified (Not applicable)	- -	-	-	Liquid (GHS definition)	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
15	Organic peroxides	Not classified (Not applicable)	- -	-	-	Organic compounds containing no bivalent -O-O- structure in the molecule	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
16	Corrosive to metals	Classification not possible	- -	-	-	No data available.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
17	Desensitized explosives	-	- -	-	-	-	-	-

HEALTH HAZARDS

Hazard class		Classification	Pictogram Signal word	Hazard statement (code)	Precautionary statement (code)	Rationale for the classification	Classification year (FY)	GHS Classification Guidance for the Japanese Government
1	Acute toxicity (Oral)	Category 4	Warning	H302	P301+P312 P264 P270 P330 P501	The following nine reports are available as LD50 values for rats: three reports of 500 mg/kg (ATSDR (2006), Environmental Risk Assessment for Chemical Substances Vol.1 (Ministry of the Environment, 2002), IARC 29 (1982)), two reports of 1,516 mg/kg (ATSDR (2006), NICNAS (2001)), approximately 2,000 mg/kg (males), > 2,000 mg/kg (females) (JECDB (Access on August 2015)), 2,138 mg/kg (NICNAS (2001)), and 1,516-2,138 mg/kg (Hazard Assessment Report (CERI, NITE, 2008), SIDS (2004)). This substance was classified in Category 4 to which the larger number of data (six reports) corresponds.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
1	Acute toxicity (Dermal)	Classification not possible	- -	-	-	Classification not possible due to lack of data.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
1	Acute toxicity (Inhalation: Gases)	Not classified (Not applicable)	- -	-	-	Liquid (GHS definition)	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
1	Acute toxicity (Inhalation: Vapours)	Category 4	Warning	H332	P304+P340 P261 P271 P312	Based on reported LC50 values for rats of 1,532 ppm (6 hours) (converted 4-hour equivalent value: 3,753 ppm) (PATTY (6th, 2012), ATSDR (2006), EHC 128 (1991)) and 961ppm (7 hours) - 1,532 ppm (6 hours) (converted 4-hour equivalent value: 2,543-3,753 ppm) (Hazard Assessment Report (CERI, NITE, 2008)), this substance was classified in Category 4. In addition, since the LC50 values were lower than the saturated vapor pressure concentration (1,935 ppm), a reference value in the units of ppm was applied as vapor without mist. By correcting the converted 4-hour equivalent value of the LC50 value which was adopted as the evidence for the previous classification, the category was revised.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
1	Acute toxicity (Inhalation: Dusts and mists)	Classification not possible	- -	-	-	Classification not possible due to lack of data.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
2	Skin corrosion/irritation	Category 2	Warning	H315	P302+P352 P332+P313 P362+P364 P264 P280 P321	There is a report that in a skin irritation test with rabbits, slight to moderate erythema and edema were observed 72 hours after 4-hour application of 0.5 mL of an undiluted solution of this substance (SIDS (2004), Hazard Assessment Report (CERI, NITE, 2008)). It is also reported that edema and blisters were observed as a result of application of this substance in humans (SIDS (2004), DFGOT vol.1 (1990)). From the above, this substance was classified in Category 2. Besides, this substance was classified as "Skin Irrit. 2 H315" in the EU CLP classification (ECHA CL Inventory (Access on September 2015)).	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
3	Serious eye damage/eye irritation	Category 2B	- Warning	H320	P305+P351+P338 P337+P313 P264	It is reported that slight irritation was observed as a result of application of two drops of an undiluted solution of this substance in the eyes of rabbits (Hazard Assessment Report (CERI, NITE, 2008), ACGIH (7th, 2001)). It is also reported that eye irritation was observed in occupational exposure (SIDS (2004), Hazard Assessment Report (CERI, NITE, 2008)). From the above, this substance was classified in Category 2B. In addition, this substance was classified as "Eye. Irrit. 2 H319" in the EU CLP classification (ECHA CL Inventory (Access on September 2015)).	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
4	Respiratory sensitization	Classification not possible	- -	-	-	Classification not possible due to lack of data.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
4	Skin sensitization	Classification not possible	- -	-	-	Classification not possible due to lack of data.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
5	Germ cell mutagenicity	Classification not possible	- -	-	-	As for in vivo, micronucleus tests with bone marrow cells of mice dosed intraperitoneally were positive and negative results, a micronucleus test with rats dosed subcutaneously was negative, a chromosome aberration test with bone marrow cells of rats dosed intraperitoneally was negative, and a replicative DNA synthesis (RDS) test with mice and DNA-binding tests in rats and mice were negative (Hazard Assessment Report (CERI, NITE, 2008), SIDS (2004), IARC 73 (1999), ATSDR (2006), NICNAS (2001)). As for in vitro, bacterial reverse mutation tests and mammalian cell genetic mutation (hgprt) tests were negative, but in a mouse lymphoma test, a chromosome aberration test, and a sister chromatid exchange test with cultured mammalian cells, positive results were reported in a metabolic activation system (Hazard Assessment Report (CERI, NITE, 2008), SIDS (2004), IARC 73 (1999), ACGIH (7th, 2001), NICNAS (2001), ATSDR (2006), NTP TR 255 (1985), JECDB (Access on August 2015)). From the above, the reproducibility of the positive finding in the in vivo bone marrow micronucleus test could not be confirmed (SIDS (2004)), therefore, this substance was classified as "Classification not possible" in accordance with the GHS Classification Guidance for the Japanese Government.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
6	Carcinogenicity	Classification not possible	- -	-	-	There is no available information for the classification on carcinogenicity in humans. As for experimental animals, carcinogenicity tests were conducted in which rats or mice were dosed by gavage with this substance for two years. In rats, males were dosed at up to doses of 60, 120 mg/kg/day where decreased body weight gain and reduced survival rate were observed, and also in mice, the same doses were given, however, no evidence of carcinogenicity was observed in either sex in either rats or mice (IARC 73 (1999), NTP TR 255 (1985)). Based on these findings, this substance was classified in Group 3 by IARC (IARC 73 (1999)). In terms of other carcinogenicity classifications, this substance was classified as "D (Not classifiable as to human carcinogenicity)" in 1991 by EPA (IRIS Summary (Access on August 2015)), and as "A4" in 1996 by ACGIH. From the above, it was classified as "Classification not possible" for this hazard class. Because the GHS Classification Guidance for the Japanese Government as the reference was changed, the classification result was changed from the previous one (Not classified).	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
7	Reproductive toxicity	Classification not possible	- -	-	-	There is no information on the reproductive toxicity in humans. As for experimental animals, in a two-generation reproduction toxicity study with rats dosed in an inhalation route, at up to doses (150, 400 ppm) where general toxicity effects (reduced body weight gain, increased liver and kidney weights, hepatocellular hypertrophy, etc.) were produced in the parental animals of the F0 and F1 generations, no effects on fertility and the next generation were observed in both generations (SIDS (2004), ATSDR (2006)). As for developmental toxicity, as a result of inhalation exposure at up to 400 ppm in pregnant rats and pregnant rabbits during the organogenesis period (rats: gestational days 6-15, rabbits: gestational days 6-18), at doses where maternal toxicity (reduced body weight gain) was observed, as developmental effects in fetuses, only a skeletal variation (delayed ossification of cervical vertebrae) was observed in rat fetuses, and no abnormality was observed in the rabbit fetuses (SIDS (2004), Hazard Assessment Report (CERI, NITE, 2008), ATSDR (2006)). In addition, pregnant rats were dosed by gavage at up to 200 mg/kg/day during the organogenesis period (gestational days 6-15), but no adverse effects were observed in either maternal animals or fetuses (SIDS (2004), Hazard Assessment Report (CERI, NITE, 2008), ATSDR (2006)). As in the above, there was no finding that clearly showed reproductive and developmental toxicity in experimental animals. However, as noted by the ATSDR, the developmental toxicity tests by the inhalation and oral routes were insufficiently described and provided a limited test result (ATSDR (2006)). Therefore, it was thought that there was a limitation to use it for classification from the viewpoint of reliability. Meanwhile, there is a description that dose-dependent morphological abnormalities were reported in the head, acrosome, and tail of sperm in a test in which male rats were given a single intraperitoneal dose at doses 50-800 mg/kg (ACGIH (7th, 2001), SIDS (2004)). Taken together, it was concluded that there was insufficient data to classify this substance as "Not classified" at the present time. Therefore, this substance was classified as "Classification not possible."	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
8	Specific target organ toxicity - Single exposure	Category 1 (liver, kidney), Category 3 (respiratory tract irritation, narcotic effects)	Danger Warning	H370 H335 H336	P308+P311 P260 P264 P270 P321 P405 P501 P304+P340 P403+P233 P261 P271 P312	This substance is a respiratory tract irritant (Hazard Assessment Report (CERI, NITE, 2008), OEL Documentations (Japan Society For Occupational Health (JSOH), 1994), ACGIH (7th, 2001), NICNAS (2001), Environmental Risk Assessment for Chemical Substances Vol. 1 (Ministry of the Environment, 2002), DFGOT Vol. 1 (1990), IARC 73 (1999), ATSDR (2006), SIDS (2004)). In humans, there are reports of narcotic effects and fatal paralysis by inhalation exposure at high concentrations, and vomiting, diarrhea, toxic hepatitis, and nephritis by ingestion (Hazard Assessment Report (CERI, NITE, 2008), OEL Documentations (Japan Society For Occupational Health (JSOH), 1994), ACGIH (7th, 2001), NICNAS (2001), Environmental Risk Assessment for Chemical Substances Vol. 1 (Ministry of the Environment, 2002)). As for experimental animals, in inhalation exposure with rats and mice, weakness, centrilobular hepatocellular necrosis, and kidney tubule damage at doses corresponding to Category 1 (surviving individual), and narcotic effects at higher concentrations were observed (Hazard Assessment Report (CERI, NITE, 2008), OEL Documentations (Japan Society For Occupational Health (JSOH), 1994), ACGIH (7th, 2001), NICNAS (2001), DFGOT Vol. 1 (1990), IARC 73 (1999), ATSDR (2006)). In oral doses with rats and mice, centrilobular hepatocellular hypertrophy, hepatocellular vacuolar degeneration, and an increase in hepatocellular proliferation with hepatocellular necrosis at doses corresponding to Category 1, and lateral position, decreased locomotor activity, closed eyelid, gait disturbance, tremors, irregular respiration, and centrilobular hepatocellular hypertrophy at doses corresponding to Category 2 were observed (SIDS (2004), DFGOT Vol. 20 (2003), NICNAS (2001), JECDB (Access on August 2015)). The finding of tremors in experimental animals was included in a narcotic effect. From the above, this substance has effects such as respiratory tract irritation, narcotic effects, and effects on the liver and kidneys. Therefore, it was classified in Category 1 (liver, kidney), Category 3 (respiratory tract irritation, narcotic effects).	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
9	Specific target organ toxicity - Repeated exposure	Category 1 (nervous system, liver, respiratory organs, blood system)	Danger	H372	P260 P264 P270 P314 P501	In humans, polyneuropathy, liver damage, irritation to the nasal cavity and respiratory tract (Hazard Assessment Report (CERI, NITE, 2008)), bone marrow hyperplasia, acute haemolytic anaemia, leukocytosis (NICNAS (2001)), etc. were seen. As for experimental animals, there is a report that in a 90-day oral toxicity test with rats dosed by gavage, centrilobular hepatocellular hypertrophy and centrilobular single cell necrosis (males) in the liver, and eosinophilic intracytoplasmic inclusion bodies in the proximal tubules of the kidney (males) were observed at or above 100 mg/kg/day (converted guidance value: 31.1 mg/kg/day), which is within the range of Category 2 (JECDB (Access on August 2015)). There is a report that in a 2-generation reproductive toxicity study with rats by the inhalation route, liver hypertrophy and effects on the kidneys (dilated renal tubules with intraluminal granular casts, intracytoplasmic granules/droplets in the proximal convoluted tubular epithelium) (males) were observed at or above 150 ppm (converted guidance value: 0.90 mg/L), which is within the range of Category 2 (SIDS (2004)). There is a report that in a 192-day inhalation toxicity study with rats, pneumonia was observed at 0.02 mg/L, which is within the range of Category 1 (Hazard Assessment Report (CERI, NITE, 2008)). As in the above, in humans, effects were seen in the nervous system, liver, respiratory organs, and haemal system. In experimental animals, effects were seen in the lungs within a guidance value range corresponding to Category 1, and in the liver and kidneys within a guidance value range corresponding to the Category 2. The kidney was not adopted as a target organ since the effects on the kidneys were considered to be specific to male rats. Therefore, this substance was classified in Category 1 (nervous system, liver, respiratory organs, haemal system).	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
10	Aspiration hazard	Classification not possible	- -	-	-	Classification not possible due to lack of data. Besides, the kinematic viscosity is calculated to be 1.014 mm2/sec (25/20 deg C) from the numerical data (viscosity: 1.324 mPa*s (25 deg C), density: 1.3059 g/mL (20 deg C)) listed on the HSDB (Access on August 2015).	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))

ENVIRONMENTAL HAZARDS

Hazard class		Classification	Pictogram Signal word	Hazard statement (code)	Precautionary statement (code)	Rationale for the classification	Classification year (FY)	GHS Classification Guidance for the Japanese Government
11	Hazardous to the aquatic environment Short term (Acute)	Category 1	Warning	H400	P273 P391 P501	From 48-hour EC50 = 0.66 mg/L for crustacea (Ceriodaphnia dubia) (NICNAS, 2001, Initial Risk Assessment (NITE, CERI, NEDO, 2007)), it was classified in Category 1.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
11	Hazardous to the aquatic environment Long term (Chronic)	Category 1	Warning	H410	P273 P391 P501	If chronic toxicity data are used, then it is classified in Category 1 due to being not rapidly degradable (a degradation rate by 28-day BOD = 0%, a degradation rate by GC = 3% (Official Bulletin of Ministry of International Trade and Industry, 1975)), and 21-day NOEC (reproduction) < 0.10 mg/L for crustacea (Daphnia magna) (Results of Aquatic Toxicity Tests of Chemicals conducted by Environment Agency in Japan (Environment Agency, 1995), Environmental Risk Assessment for Chemical Substances vol. 1 (Ministry of the Environment, 2002), Initial Risk Assessment (NITE, CERI, NEDO, 2007)). If acute toxicity data are used for a trophic level for which chronic toxicity data are not obtained, then it is classified in Category 2 due to being not rapidly degradable and 96-hour LC50 = 1.16 mg/L for fish (Oncorhynchus mykiss) (SIDS, 2004). By drawing a comparison between the above results, it was classified in Category 1.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))
12	Hazardous to the ozone layer	Classification not possible	- -	-	-	No data available.	FY2015	GHS Classification Guidance for the Japanese Government (FY2013 revised edition (Ver. 1.1))

NOTE:

GHS Classification Result by the Japanese Government is intended to provide a reference for preparing a GHS label or SDS for users. To include the same classification result in a label or SDS for Japan is NOT mandatory.
Users can cite or copy this classification result when preparing a GHS label or SDS. Please be aware, however, that the responsibility for a label or SDS prepared by citing or copying this classification result lies with users.
This GHS classification was conducted based on the information sources and the guidance for classification and judgement which are described in the GHS Classification Guidance for the Japanese Government etc. Using other literature, test results etc. as evidence and including different content from this classification result in a label or SDS are allowed.
Hazard statement and precautionary statement will show by hovering the mouse cursor over a code in the column of "Hazard statement" and "Precautionary statement," respectively. In the excel file, both the codes and statements are provided.
A blank or "-" in the column of "Classification" denotes that a classification for the hazard class was not conducted in the year.
An asterisk “*” in the column of “Classification” denotes that “Not classified (or No applicable)” and/or “Classification not possible” is applicable. Details are described in the column of “Rationale for the classification”. If no English translation is available for “Rationale for the classification,” please refer to the Japanese version of the results.

To GHS Information