Monensin : Cluster information --- DoBISCUIT

Entry name	Monensin
PKS Type	TypeI modular
Starter Unit	malonyl-CoA
Chain Length	13
Sugar Unit	none
Classification	Polyether
Activity	Antibacterial Ionophore Antiprotozoal
Composition	A: C₃₆H₆₂O₁₁ B: C₃₅H₆₀O₁₁ C: C₃₇H₆₄O₁₁

Organism	Streptomyces cinnamonensis
Strain	ATCC 15413
Contig	AF440781

Monen_00150 monAI	0	malonyl-CoA
Monen_00150 monAI	1	methylmalonyl-CoA
Monen_00160 monAII	2	methylmalonyl-CoA
Monen_00170 monAIII	3	malonyl-CoA
Monen_00170 monAIII	4	methylmalonyl-CoA
Monen_00180 monAIV	5	A: ethylmalonyl-CoA B: methylmalonyl-CoA
Monen_00180 monAIV	6	malonyl-CoA
Monen_00190 monAV	7	methylmalonyl-CoA
Monen_00190 monAV	8	malonyl-CoA
Monen_00200 monAVI	9	malonyl-CoA
Monen_00260 monAVII	10	methylmalonyl-CoA
Monen_00250 monAVIII	11	methylmalonyl-CoA
Monen_00250 monAVIII	12	methylmalonyl-CoA
Monen_00090 ksX	13
Monen_00080 acpX	13

KSQ	17..381

AT	553..899

ACP	977..1050

KS	1079..1455

AT	1615..1930

dh	1978..2167

kr	2508..2721

ACP	2818..2888

KS	34..408

AT	564..881

DH	927..1090

ER	1445..1753

KR	1763..1943

ACP	2049..2119

KS	34..405

AT	609..932

DH	981..1198

KR	1576..1756

ACP	1873..1943

KS	1975..2351

AT	2507..2824

DH	2873..3037

ER	3364..3671

KR	3681..3861

ACP	3971..4041

KS	60..433

AT	593..910

DH	959..1133

KR	1449..1629

ACP	1728..1798

KS	1833..2209

AT	2377..2696

DH	2744..2910

ER	3239..3545

KR	3555..3735

ACP	3877..3950

KS	34..408

AT	563..879

DH	927..1097

KR	1482..1662

ACP	1764..1834

KS	1856..2232

AT	2398..2720

DH	2768..2935

ER	3302..3608

KR	3618..3798

ACP	3943..4013

Production of 26-deoxymonensins A and B by Streptomyces cinnamonensis in the presence of Metyrapone.: Pospisil S, Sedmera P, Havlicek V, Tax J
[PMID: 8017935]Appl Environ Microbiol. 60 (1994) 1561-4
Engineering of complex polyketide biosynthesis--insights from sequencing of the monensin biosynthetic gene cluster.: Leadlay PF, Staunton J, Oliynyk M, Bisang C, Cortes J, Frost E, Hughes-Thomas ZA, Jones MA, Kendrew SG, Lester JB, Long PF, McArthur HA, McCormick EL, Oliynyk Z, Stark CB, Wilkinson CJ
[PMID: 11774001]J Ind Microbiol Biotechnol. 27 (2001) 360-7
Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization.: Oliynyk M, Stark CB, Bhatt A, Jones MA, Hughes-Thomas ZA, Wilkinson C, Oliynyk Z, Demydchuk Y, Staunton J, Leadlay PF
[PMID: 12940979]Mol Microbiol. 49 (2003) 1179-90
Accumulation of an E,E,E-triene by the monensin-producing polyketide synthase when oxidative cyclization is blocked.: Bhatt A, Stark CB, Harvey BM, Gallimore AR, Demydchuk YA, Spencer JB, Staunton J, Leadlay PF
[PMID: 16217816]Angew Chem Int Ed Engl. 44 (2005) 7075-8
Evidence for the role of the monB genes in polyether ring formation during monensin biosynthesis.: Gallimore AR, Stark CB, Bhatt A, Harvey BM, Demydchuk Y, Bolanos-Garcia V, Fowler DJ, Staunton J, Leadlay PF, Spencer JB
[PMID: 16632258]Chem Biol. 13 (2006) 453-60
Evidence that a novel thioesterase is responsible for polyketide chain release during biosynthesis of the polyether ionophore monensin.: Harvey BM, Hong H, Jones MA, Hughes-Thomas ZA, Goss RM, Heathcote ML, Bolanos-Garcia VM, Kroutil W, Staunton J, Leadlay PF, Spencer JB
[PMID: 16897798]Chembiochem. 7 (2006) 1435-42
Identification of NanE as the thioesterase for polyether chain release in nanchangmycin biosynthesis.: Liu T, You D, Valenzano C, Sun Y, Li J, Yu Q, Zhou X, Cane DE, Deng Z
[PMID: 16984884]Chem Biol. 13 (2006) 945-55
Allosteric regulation of epoxide opening cascades by a pair of epoxide hydrolases in monensin biosynthesis.: Minami A, Ose T, Sato K, Oikawa A, Kuroki K, Maenaka K, Oguri H, Oikawa H
[PMID: 24320215]ACS Chem Biol. 9 (2014) 562-9
Predicted incorporation of non-native substrates by a polyketide synthase yields bioactive natural product derivatives.: Bravo-Rodriguez K, Ismail-Ali AF, Klopries S, Kushnir S, Ismail S, Fansa EK, Wittinghofer A, Schulz F, Sanchez-Garcia E
[PMID: 25044264]Chembiochem. 15 (2014) 1991-7