Kirromycin : Cluster information
Compound
| Entry name | Kirromycin |
|---|---|
| PKS Type | Trans-AT PKS-NRPS hybrid |
| Starter Unit | acetyl-CoA |
| Chain Length | 15 |
| Sugar Unit | none |
| Classification | Peptide-Polyketide Hybrid |
| Activity | Antibacterial |
| Composition | C43H60N2O12 |
Original source
| Organism | Streptomyces collinus |
|---|---|
| Strain | Tü365 |
| Contig | AM746336 |
PKS/NRPS Module
| Kirro_00220 kirAI | 0 |  | |
| 1 |  | ||
| 2 |  | ||
| Kirro_00230 kirAII | 2 |  | |
| 3 |  | ||
| 4 | | ||
| 5 |  | ||
| Kirro_00240 kirAIII | 6 |  | glycine |
| 7 | | ||
| Kirro_00250 kirAIV | 7 |  | |
| 8 |  | ||
| 9 | | ||
| 10 | | ||
| 11 | | ||
| 12 | | ||
| Kirro_00260 kirAV | 12 | | |
| 13 |  | ||
| Kirro_00270 kirAVI | 14 |  | methylmalonyl-CoA |
| 15 |  | malonyl-CoA not conserved HAFHS(H->T) | |
| Kirro_00290 kirB | 16 | | beta-alanine |
| Kirro_00300 kirCI | 17 |  | malonyl-CoA |
| Kirro_00340 kirCII | 18 |  | ethylmalonyl-CoA |
Reference
- Molecular analysis of the kirromycin biosynthetic gene cluster revealed beta-alanine as precursor of the pyridone moiety.
- Weber T, Laiple KJ, Pross EK, Textor A, Grond S, Welzel K, Pelzer S, Vente A, Wohlleben W[PMID: 18291322]Chem Biol. 15 (2008) 175-88
- The kirromycin gene cluster of Streptomyces collinus Tu 365 codes for an aspartate-alpha-decarboxylase, KirD, which is involved in the biosynthesis of the precursor beta-alanine.
- Laiple KJ, Hartner T, Fiedler HP, Wohlleben W, Weber T[PMID: 19609288]J Antibiot (Tokyo). 62 (2009) 465-8
- The phosphopantetheinyl transferase KirP activates the ACP and PCP domains of the kirromycin NRPS/PKS of Streptomyces collinus Tu 365.
- Pavlidou M, Pross EK, Musiol EM, Kulik A, Wohlleben W, Weber T[PMID: 21401713]FEMS Microbiol Lett. 319 (2011) 26-33
- Supramolecular templating in kirromycin biosynthesis: the acyltransferase KirCII loads ethylmalonyl-CoA extender onto a specific ACP of the trans-AT PKS.
- Musiol EM, Hartner T, Kulik A, Moldenhauer J, Piel J, Wohlleben W, Weber T[PMID: 21513880]Chem Biol. 18 (2011) 438-44
- Poly specific trans-acyltransferase machinery revealed via engineered acyl-CoA synthetases.
- Koryakina I, McArthur J, Randall S, Draelos MM, Musiol EM, Muddiman DC, Weber T, Williams GJ[PMID: 23083014]ACS Chem Biol. 8 (2013) 200-8
- The AT(2) domain of KirCI loads malonyl extender units to the ACPs of the kirromycin PKS.
- Musiol EM, Greule A, Hartner T, Kulik A, Wohlleben W, Weber T[PMID: 23828654]Chembiochem. 14 (2013) 1343-52
- Complete genome sequence of the kirromycin producer Streptomyces collinus Tu 365 consisting of a linear chromosome and two linear plasmids.
- Ruckert C, Szczepanowski R, Albersmeier A, Goesmann A, Iftime D, Musiol EM, Blin K, Wohlleben W, Puhler A, Kalinowski J, Weber T[PMID: 24140291]J Biotechnol. 168 (2013) 739-40
- Reprogramming acyl carrier protein interactions of an Acyl-CoA promiscuous trans-acyltransferase.
- Ye Z, Musiol EM, Weber T, Williams GJ[PMID: 24726832]Chem Biol. 21 (2014) 636-46
- Comparative analysis of the substrate specificity of trans- versus cis-acyltransferases of assembly line polyketide synthases.
- Dunn BJ, Watts KR, Robbins T, Cane DE, Khosla C[PMID: 24871074]Biochemistry. 53 (2014) 3796-806
- Discovery of a new family of Dieckmann cyclases essential to tetramic acid and pyridone-based natural products biosynthesis.
- Gui C, Li Q, Mo X, Qin X, Ma J, Ju J[PMID: 25621700]Org Lett. 17 (2015) 628-31
Data download
| Compound name | Genomic nucleotide sequence |
Nucleotide sequences of the coding regions | Translation of the coding sequences | List of genes/ORFs | GBK | |||||||
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 Kirromycin |
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History
- 2016-01-27[Update]
- 2014-06-25[Update]
- 2013-12-27[Update]
- 2012-10-02[Update]
- 2012-08-03[Release]
