Neocarzinostatin : Cluster information
Compound
Entry name | Neocarzinostatin |
---|---|
Moiety description | A: enediyne core B: naphthoic acid moiety |
PKS Type | A: TypeI iterative B: TypeI iterative |
Starter Unit | A: acetyl-CoA B: acetyl-CoA |
Chain Length | A: 8 B: 6 |
Sugar Unit | N-methyl-D-fucosamine |
Classification | Enediyne |
Activity | Antibiotic Antitumor |
Composition | C35H33NO12 |
Original source
Organism | Streptomyces carzinostaticus |
---|---|
Strain | ATCC 15944 |
Contig | AY117439 |
PKS/NRPS Module
A | Ncarz_00330 ncsE | 1 | acetyl-CoA malonyl-CoA | |
---|---|---|---|---|
B | Ncarz_00070 ncsB | 1 | acetyl-CoA malonyl-CoA |
Reference
- A genomics-guided approach for discovering and expressing cryptic metabolic pathways.
- Zazopoulos E, Huang K, Staffa A, Liu W, Bachmann BO, Nonaka K, Ahlert J, Thorson JS, Shen B, Farnet CM[PMID: 12536216]Nat Biotechnol. 21 (2003) 187-90
- Neocarzinostatin naphthoate synthase: an unique iterative type I PKS from neocarzinostatin producer Streptomyces carzinostaticus.
- Sthapit B, Oh TJ, Lamichhane R, Liou K, Lee HC, Kim CG, Sohng JK[PMID: 15147895]FEBS Lett. 566 (2004) 201-6
- The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases.
- Liu W, Nonaka K, Nie L, Zhang J, Christenson SD, Bae J, Van Lanen SG, Zazopoulos E, Farnet CM, Yang CF, Shen B[PMID: 15797213]Chem Biol. 12 (2005) 293-302
- Characterization of NcsB2 as a promiscuous naphthoic acid/coenzyme A ligase integral to the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin.
- Cooke HA, Zhang J, Griffin MA, Nonaka K, Van Lanen SG, Shen B, Bruner SD[PMID: 17539640]J Am Chem Soc. 129 (2007) 7728-9
- A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis.
- Zhang J, Van Lanen SG, Ju J, Liu W, Dorrestein PC, Li W, Kelleher NL, Shen B[PMID: 18223152]Proc Natl Acad Sci U S A. 105 (2008) 1460-5
- Regiospecific O-methylation of naphthoic acids catalyzed by NcsB1, an O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin.
- Luo Y, Lin S, Zhang J, Cooke HA, Bruner SD, Shen B[PMID: 18387946]J Biol Chem. 283 (2008) 14694-702
- Molecular basis of substrate promiscuity for the SAM-dependent O-methyltransferase NcsB1, involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin.
- Cooke HA, Guenther EL, Luo Y, Shen B, Bruner SD[PMID: 19702337]Biochemistry. 48 (2009) 9590-8
- Characterization of the epoxide hydrolase NcsF2 from the neocarzinostatin biosynthetic gene cluster.
- Lin S, Horsman GP, Shen B[PMID: 20704329]Org Lett. 12 (2010) 3816-9
- In vivo characterization of NcsB3 to establish the complete biosynthesis of the naphthoic acid moiety of the neocarzinostatin chromophore.
- Hang VT, Oh TJ, Yamaguchi T, Sohng JK[PMID: 20735485]FEMS Microbiol Lett. 311 (2010) 119-25
- Predictive model for epoxide hydrolase-generated stereochemistry in the biosynthesis of nine-membered enediyne antitumor antibiotics.
- Horsman GP, Lechner A, Ohnishi Y, Moore BS, Shen B[PMID: 23844627]Biochemistry. 52 (2013) 5217-24
- Enediyne polyketide synthases stereoselectively reduce the beta-ketoacyl intermediates to beta-D-hydroxyacyl intermediates in enediyne core biosynthesis.
- Ge HM, Huang T, Rudolf JD, Lohman JR, Huang SX, Guo X, Shen B[PMID: 25019332]Org Lett. 16 (2014) 3958-61
- Insights into the programmed ketoreduction of partially reducing polyketide synthases: stereo- and substrate-specificity of the ketoreductase domain.
- Soehano I, Yang L, Ding F, Sun H, Low ZJ, Liu X, Liang ZX[PMID: 25238086]Org Biomol Chem. 12 (2014) 8542-9
Data download
History
- 2016-01-27[Update]
- 2014-06-25[Update]
- 2013-12-27[Release]