NITE - Chemical Management Field

Latest GHS Classification Results by the Japanese Government (edited by NITE)

GENERAL INFORMATION

Item	Information
CAS RN	1912-24-9
Chemical Name	Atrazine
Substance ID	m-nite-1912-24-9_v1
Download of Excel format	Excel file

REFERENCE INFORMATION

Item	Information
Guidance used for the classification (External link)	To Guidance List
UN GHS document (External link)	To UN GHS document
FAQ（GHS classification results by the Japanese Government）	To FAQ
List of Information Sources (Excel file)	List of Information Sources
List of Definitions/Abbreviations	Definitions/Abbreviations
Sample Label by MHLW (External link)	MHLW Website (in Japanese Only)
Sample SDS by MHLW (External link)	MHLW Website (in Japanese Only)
OECD/eChemPortal (External link)	To OECD/eChemPortal (External link)

PHYSICAL HAZARDS

Hazard class		Classification	Pictogram Signal word	Hazard statement (code)	Precautionary statement (code)	Rationale for the classification	Classification year (FY)	GHS Classification Guidance for the Japanese Government
1	Explosives	Not classified (Not applicable)	- -	-	-	There are no chemical groups associated with explosive properties present in the molecule.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
2	Flammable gases	Not classified (Not applicable)	- -	-	-	Solid (GHS definition)	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
3	Aerosols	Not classified (Not applicable)	- -	-	-	Not aerosol products.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
4	Oxidizing gases	Not classified (Not applicable)	- -	-	-	Solid (GHS definition)	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
5	Gases under pressure	Not classified (Not applicable)	- -	-	-	Solid (GHS definition)	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
6	Flammable liquids	Not classified (Not applicable)	- -	-	-	Solid (GHS definition)	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
7	Flammable solids	Not classified	- -	-	-	Based on the information of nonflammable (IUCLID (2000) test method: Directive 84/449/EEC A10), the substance is classified as "Not classified".	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
8	Self-reactive substances and mixtures	Not classified (Not applicable)	- -	-	-	There are no chemical groups present in the molecule associated with explosive or self-reactive properties.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
9	Pyrophoric liquids	Not classified (Not applicable)	- -	-	-	Solid (GHS definition)	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
10	Pyrophoric solids	Classification not possible	- -	-	-	Insufficient data available. It is reported as "No self ignition" (IUCLID (2000)) measured by the method of Directive 84/449/EEC A16, but no basis of pyrophoric properties in GHS is derived from this data. Therefore it is classified as "Classification not possible".	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
11	Self-heating substances and mixtures	Classification not possible	- -	-	-	Insufficient data available. It is reported as non flammable measured by the method of Directive 84/449/EEC A 10 (IUCLID (2000)), but no basis of self-heating properties in GHS is derived from this data. Therefore it is classified as "Classification not possible".	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
12	Substances and mixtures which, in contact with water, emit flammable gases	Not classified (Not applicable)	- -	-	-	The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
13	Oxidizing liquids	Not classified (Not applicable)	- -	-	-	Solid (GHS definition)	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
14	Oxidizing solids	Not classified (Not applicable)	- -	-	-	The substance is an organic compound containing chlorine which is chemically bonded only to carbon.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
15	Organic peroxides	Not classified (Not applicable)	- -	-	-	Organic compounds containing no bivalent -O-O- structure	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
16	Corrosive to metals	Classification not possible	- -	-	-	Test methods applicable to solid substances are not available. As relevant note, it is reported as "noncorrosive to equipment and metal surfaces" (HSDB (2004)).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
17	Desensitized explosives	-	- -	-	-	-	-	-

HEALTH HAZARDS

Hazard class		Classification	Pictogram Signal word	Hazard statement (code)	Precautionary statement (code)	Rationale for the classification	Classification year (FY)	GHS Classification Guidance for the Japanese Government
1	Acute toxicity (Oral)	Category 4	Warning	H302	P301+P312 P264 P270 P330 P501	Eight LD50 values of 3250, 3000 mg/kg (both from Registration and application document of pesticide (2002)), 2000, 670, 740, 2300 mg/kg (all from IARC 73 (1999)), 1471 and 1212 mg/kg (both from ATSDR (2003)) for rats were reported. The five of them corresponded to Category 4, and the other three corresponded to "Not classified" in the JIS classification. The substance was classified as Category 4, as most of the data corresponded to it.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
1	Acute toxicity (Dermal)	Not classified	- -	-	-	Three LD50 values of > 5000, > 2000 mg/kg (both from Registration and application document of pesticide (2002)) and > 3100 mg/kg (PATTY, 5th (2001))) for rats and the one LD50 value of 9300 mg/kg (PATTY, 5th (2001) for rabbits were reported. All of them correspond to "Not classified" in the JIS classification (corresponding to Category 5 or "Not classified" in the UN-GHS classification).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
1	Acute toxicity (Inhalation: Gases)	Not classified (Not applicable)	- -	-	-	Solid (GHS definition)	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
1	Acute toxicity (Inhalation: Vapours)	Classification not possible	- -	-	-	No data available.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
1	Acute toxicity (Inhalation: Dusts and mists)	Not classified	- -	-	-	Based on the rat LC50 values of > 5.148 mg/L/4hrs and > 5.82 mg/L/4hrs (Registration and application document of pesticide (2002)), > 2.84 mg/L/4hrs and 8 mg/L/4hrs (both from PATTY, 5th (2001)), the substance was classified as "Not classified" in the JIS classification (corresponding to Category 5 or "Not classified" in the UN-GHS classification). The test concentration was higher than the saturated vapor pressure concentration (3.35E-06 mg/L), the criterion values for dust/mist were adopted.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
2	Skin corrosion/irritation	Not classified	- -	-	-	In the rabbit test of technical product of pesticide, 24-hour application of 0.5 g of the substance caused slight irritation (Registration and application document of pesticide (1989)). In another rabbit test, 24-hour application of 0.5 g of the substance caused no irritation (IUCLID (2000)). The substance was minimally irritating to rabbit skin (PATTY, 5th (2001)). Based on these documents, the substance was classified as "Not classified" in the JIS classification (corresponding to Category 3 or "Not classified" in the UN-GHS classification).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
3	Serious eye damage/eye irritation	Category 2B	- Warning	H320	P305+P351+P338 P337+P313 P264	In the rabbit test, instillation of 0.1 g of the technical product caused no irritation, and caused slight irritation with 50% water dispersible formulation (wettable powder) of the substance (Registration and application document of pesticide (1989)). And it was reported that the substance caused mild irritation to rabbit eye (PATTY, 5th (2001)). Based on these documents, the substance was classified as Category 2B.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
4	Respiratory sensitization	Classification not possible	- -	-	-	No data available.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
4	Skin sensitization	Category 1	Warning	H317	P302+P352 P333+P313 P362+P364 P261 P272 P280 P321 P501	In the guinea pig maximization test (OECD TG 406) of technical product of pesticide, skin reaction was observed in 17 of 20 animals, and positive rate was 85% (Registration and application document of pesticide (1997)). In other guinea pig maximization test (OECD TG 406, GLP-compliant), 65 - 70% of animals revealed skin reaction (positive rate), and it was reported that the substance was classified as a sensitizer (IUCLID (2000)). Based on these documents, the substance was classified as Category 1. As relevant information, 50% water dispersible formulation (wettable powder) of the substance did not cause sensitization in the Buehler test (Registration and application document of pesticide (1997)). And in the repeated patch test that 50 human volunteers were dermally exposed, no signs of dermal reaction were observed and resulted as "not sensitizing" (IUCLID (2000)).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
5	Germ cell mutagenicity	Classification not possible	- -	-	-	The dominant lethal test using mice orally administered (in vivo inheritable mutagenicity test in germ cells) resulted weakly positive (ATSDR (2003)). However, since the test was conducted only at one dose level, therefore, the evaluation was impossible. As for the in vivo mutagenicity test in somatic cells, in the micronucleus test using bone marrow cell from mice orally administered, positive results in females and negative in males, showing a contradictory result, were noted (ATSDR (2003)). However, there was no description about the sex difference, and only one animal was used in the negative control group, therefore, the evaluation was impossible. In the chromosome aberration test using bone marrow cell obtained from mice orally administered via drinking water for 90 days at the dose level of 20 ppm, negative results were reported (ATSDR (2003)). However, it was difficult to evaluate the validity of the dose level of 20 ppm and the effects by the short-term high concentration exposure. Although another multiple in vivo test data, such as negative result in the micronucleus test using bone marrow from mice intraperitoneally administered, and negative results in the stomach, liver and kidney, but positive in the lung in the DNA damage test (in vivo genotoxicity test in somatic cells) using rats orally administered (ATSDR (2003)), were obtained, both of them were unable to be evaluated for classification. Therefore, the substance was classified as "Classification not possible". As relevant information, as for in vitro studies, negative results were reported in the Ames test (NTP DB (Access on Jun. 2009)) and chromosome aberration test using human lymphocytes and CHO cells (IARC 73 (1999)).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
6	Carcinogenicity	Classification not possible	- -	-	-	The substance was classified as "Classification not possible" based on the criteria of "Group 3" in the carcinogenicity assessment of the IARC (IARC 73 (1999)) and "A4" in the assessment of the ACGIH (ACGIH (2001)). As relevant information, no increase in the treatment-related incidence of tumors were reported in the 91-week dietary administration test in mice (IARC 73 (1999)). In the 104-week dietary administration test in rats, significant effects were not indicated in any dose groups (IARC 73 (1999)). In the maximum 106-week dietary administration test in SD rats, significantly increase in the incidences of fibroadenoma and adenocarcinoma of the mammary gland in females were reported (IARC 73 (1999)).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
7	Reproductive toxicity	Category 2	Warning	H361	P308+P313 P201 P202 P280 P405 P501	In the two generation study in rats that administrated by feeding from 10 weeks before mating, at the maximum tolerated dose (500 ppm), any adverse effects on the reproductive performance or on the prenatal and postnatal parameters were not caused, no treatment-related malformations, embryotoxicity or specific fetotoxicity were also observed (IUCLID (2000)). In the oral administration test during the organogenetic period in rabbits, abortions in 2/19 dams, increased incidence of fetal resorptions and post-implantation loss were observed at the dose (75 mg/kg) that reduced feed consumption and reduced body weight was observed in dams (IARC 73 (1999)). Therefore, the substance was classified as Category 2. In addition, in the administration test during the organogenetic period in rabbits, there were no dose-related increases in the incidence of malformations.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
8	Specific target organ toxicity - Single exposure	Category 2 (nervous system)	Warning	H371	P308+P311 P260 P264 P270 P405 P501	In multiple acute oral toxicity studies, sedation, dyspnea, exophthalmos etc. were reported within the dose range of 600 - 6000 mg/kg, and hypoactivity, ataxia, salivation, etc. were reported in another study within the dose range of 2000 - 5500 mg/kg for rats. For mice, hypoactivity, ptosis, ataxia, tremor etc. were reported within the dose range of 444 - 1332 mg/kg, and sedation, dyspnea etc. were reported in another study within the dose range of 1670 - 6000 mg/kg (IUCLID (2000)). While, in the study about neurological effects after single oral administration of 100 mg/kg to rats, the effects on the cerebellum containing reduction in cerebellar activity and abolish of the evoked spiked activity of Purkinje cells following stimulation of the radial nerves etc. were reported (ATSDR (2000)). As described above, the effects on the nervous system were suggested at the dose range equivalent to Category 2 of the guidance values or higher, judging from the signs observed in the acute oral toxicity studies. In consideration with the study report indicating the effects on the cerebellum as well, the substance was classified as Category 2 (nervous system). Otherwise, no serious toxic effects were found either at the concentration of 5.1 mg/L (as dusts) in inhalation route or at 2000 mg/kg in dermal route (IUCLID (2000)).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
9	Specific target organ toxicity - Repeated exposure	Category 2 (heart, liver, kidney)	Warning	H373	P260 P314 P501	In the 7-day or 14-day oral administration study in rats treated with 100 - 600 mg/kg/day (converted dose levels as that of 90-day study: 15.6 - 93.3 mg/kg/day), it was reported that hepatotoxicity such as increase of serum GPT and alkaline phosphatase activities, and degeneration of the smooth endoplasmic reticulum, lipid drop accumulation and swollen mitochondria as electron micrographic findings were observed in all treatment groups except for the lowest dose group, and that renal toxicity containing proteinuria, reduced creatinine clearance and increased urinary electrolyte output were observed in all treatment groups (IARC 73 (1999)). Since the test dose levels ranged within Category 2 of the guidance values, the substance was classified as Category 2 (liver, kidney). While in the one-year feeding study in dogs, the most significant effect was the cardiopathy which was characterized by discrete myocardial degeneration, which was the most prominently found in animals receiving 1000 ppm (ca. 34 mg/kg/day). In the study, cardiac toxicity such as ascites, cachexia, labored/shallow breathing, and abnormal changes on the electrocardiograph (ECG) were reported, and dilatation of the right atrium as gross pathological examination, microscopically atrophy and myelosis were reported (IRIS (2004)). Based on the information, the substance was classified as Category 2 (heart).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
10	Aspiration hazard	Classification not possible	- -	-	-	No data available.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）

ENVIRONMENTAL HAZARDS

Hazard class		Classification	Pictogram Signal word	Hazard statement (code)	Precautionary statement (code)	Rationale for the classification	Classification year (FY)	GHS Classification Guidance for the Japanese Government
11	Hazardous to the aquatic environment Short term (Acute)	Category 1	Warning	H400	P273 P391 P501	Classified into Category 1 from its 96h-EbC50 = 0.147 mg/L for algae (Skeletonema costatum) (ECETOC TR91, 2003).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
11	Hazardous to the aquatic environment Long term (Chronic)	Category 1	Warning	H410	P273 P391 P501	Classified into Category 1 since its acute toxicity is Category 1 and it is not rapidly degradable (BOD degradation rate: 1% (Biodegradation and Bioconcentration of Existing Chemical Substances under the Chemical Substances Control Law, 2002)).	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）
12	Hazardous to the ozone layer	Classification not possible	- -	-	-	This substance is not listed in Annexes to the Montreal Protocol.	FY2010	GHS Classification Guidance by the Japanese Government （July, 2010）

NOTE:

GHS Classification Result by the Japanese Government is intended to provide a reference for preparing a GHS label or SDS for users. To include the same classification result in a label or SDS for Japan is NOT mandatory.
Users can cite or copy this classification result when preparing a GHS label or SDS. Please be aware, however, that the responsibility for a label or SDS prepared by citing or copying this classification result lies with users.
This GHS classification was conducted based on the information sources and the guidance for classification and judgement which are described in the GHS Classification Guidance for the Japanese Government etc. Using other literature, test results etc. as evidence and including different content from this classification result in a label or SDS are allowed.
Hazard statement and precautionary statement will show by hovering the mouse cursor over a code in the column of "Hazard statement" and "Precautionary statement," respectively. In the excel file, both the codes and statements are provided.
A blank or "-" in the column of "Classification" denotes that a classification for the hazard class was not conducted in the year.
An asterisk “*” in the column of “Classification” denotes that “Not classified (or No applicable)” and/or “Classification not possible” is applicable. Details are described in the column of “Rationale for the classification”. If no English translation is available for “Rationale for the classification,” please refer to the Japanese version of the results.

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