Aureo_00040 Aureo_00040   Aureo_00060 Aureo_00060

Aureo_00050 : CDS information

close this sectionLocation

Organism
StrainHKI-227
Entry nameAureothin
Contig
Start / Stop / Direction11,811 / 13,832 / + [in whole cluster]
11,811 / 13,832 / + [in contig]
Location11811..13832 [in whole cluster]
11811..13832 [in contig]
TypeCDS
Length2,022 bp (673 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative aminodeoxychorismate synthase
putative 4-amino-4-deoxychorismate synthase
Product (GenBank)PABA synthase
GeneaurG
pabAB
Gene (GenBank)aurG
EC number2.6.1.85
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[14700630] Iteration as programmed event during polyketide assembly; molecular analysis of the aureothin biosynthesis gene cluster. (Chem Biol. , 2003)
comment
aureothin生合成gene clusterの同定論文。

AurG: PABA synthase

AurG(695aa)はbifunctional PABA synthasesのグループに高い相同性がある。
PKS starter unitであるPNBA(PABA derivative)の合成に関与すると考えられている。
Related Reference
ACC
P72539
PmId
[9044253] Identification and analysis of genes from Streptomyces pristinaespiralis encoding enzymes involved in the biosynthesis of the 4-dimethylamino-L-phenylalanine precursor of pristinamycin I. (Mol Microbiol. , 1997)
comment
27th(P72539) 48%, 1e-154
Streptomyces pristinaespiralis_papA
PapA

papA, papB, papC, papMはpristinamycin I (PI) precursor 4-dimethylamino-L-phenylalanine (DMPAPA)生合成に関連すると示唆される。
papA mutantはPI非産生、DMPAPAを添加すると産生回復する。
ACC
P32483
PmId
[11782498] The candicidin gene cluster from Streptomyces griseus IMRU 3570. (Microbiology. , 2002)
comment
33rd(P32483) 49%, 1e-152
Streptomyces griseus_pab
Para-aminobenzoate synthase(EC 2.6.1.85)

aureothin生合成gene cluster同定論文[PMID: 14700630]において、pabABに似ているStreptomyces griseus_PabABのref.として、このentryの登録論文[PMID: 8472954]が挙げられている。
pabABの置換はcandicidin産生を廃止し、これはPABAの添加によってのみ回復した。
ACC
Q56151
PmId
[8704974] A role for pabAB, a p-aminobenzoate synthase gene of Streptomyces venezuelae ISP5230, in chloramphenicol biosynthesis. (Microbiology. , 1996)
comment
44th(Q56151) 47%, 1e-140
Streptomyces venezuelae_pabAB
P-aminobenzoic acid synthase

pabABのサブクローニング、シークエンス、配列解析。
pabABの不活化はPABAの競合阻害剤であるsulfanilamideの抵抗性を減らし、PabAB reactionを介するchloramphenicol産生をブロックする。

close this sectionSequence

selected fasta
>putative aminodeoxychorismate synthase [PABA synthase]
MYGKEPTVLVNDDPSWPELDPAMFDCVVVSPGPGRPQHPADLGFCQEVLDRWTDTPVLGV
CLGHQAIAHHHGADVAEGTPKHGHLARVRHGGRDLFDGLPQNFTAVRYHSLRAAEPLPEE
LEATAWAEDGTVMALRHRTLPRWGVQFHPESIASEWGAQLLQNFAKLVGAEYAAAPRERA
RWAAPLEDAPAEAPELRPVFEVIDRAVDTAELFTTLYSASAGCFWLDSSAPGIGPARFSF
LGDDSGPLAETLTYRTGENTVAVRDAAGERAEPGTIFEVLQQRLRDRRLSTPELPFDLNG
GYVGYFGYELKGECGSPNVHRSETPDAVWTFADRLIAVDHLEEKTYVVAVSGTGPGEEDA
ARAWVTETARRARELPDPAPSEAPAPAATAPSEQVDIARDRGQYVSDIKDCLDELHSGES
YEICLTNRIRLPELKDPLGYHLLLRRLNPAPYGAFLRHEGVSVVCSSPERFLRIDRDGVV
ESRPIKGTAPRGEDPAADEALRVSLAASAKTRAENLMIVDLLRNDLGRVCEVGSVDVPAY
MVTETYATVHQLVTTVRGRLRHDVDPVACVRACFPGGSMTGAPKLRTMAIIDRLEQEARG
IYSGTIGYFGLSGAADLNIVIRTAVSTGTGTVIGTGGAIVLDSDPDEEFDEVLLKGHALV
RAHGLLEGAAEAP
selected fasta
>putative aminodeoxychorismate synthase [PABA synthase]
GTGTACGGCAAGGAACCGACGGTCCTCGTCAACGACGACCCGTCGTGGCCGGAGCTGGAC
CCGGCGATGTTCGACTGCGTCGTCGTCTCGCCCGGCCCGGGCCGCCCCCAGCACCCGGCC
GACCTCGGCTTCTGCCAGGAGGTCCTGGACCGCTGGACGGACACCCCGGTCCTCGGGGTG
TGCCTGGGGCACCAGGCGATCGCCCACCACCACGGCGCGGACGTGGCCGAGGGCACGCCG
AAGCACGGTCACCTGGCGCGGGTCCGGCACGGCGGGCGGGACCTCTTCGACGGCCTGCCG
CAGAACTTCACCGCGGTGCGCTACCACTCGCTGCGGGCCGCCGAACCCCTGCCGGAGGAG
CTGGAGGCCACCGCCTGGGCCGAGGACGGCACCGTCATGGCGCTGCGGCACCGCACGCTG
CCCCGCTGGGGCGTGCAGTTCCACCCGGAGTCCATCGCGAGCGAGTGGGGCGCCCAACTG
CTCCAGAACTTCGCGAAGCTCGTCGGCGCCGAGTACGCCGCCGCGCCCCGGGAACGGGCG
CGGTGGGCCGCCCCGTTGGAGGACGCCCCCGCGGAGGCGCCGGAACTGCGGCCCGTGTTC
GAGGTGATCGACCGGGCCGTGGACACCGCGGAGCTGTTCACCACGCTGTACTCCGCGAGC
GCCGGCTGCTTCTGGCTCGACAGCAGCGCGCCGGGGATCGGGCCGGCCCGGTTCTCGTTC
CTCGGGGACGACTCCGGCCCCCTCGCCGAGACGCTGACGTACCGGACCGGGGAGAACACG
GTCGCGGTGCGCGACGCCGCCGGTGAACGGGCCGAGCCCGGCACCATCTTCGAGGTGCTC
CAGCAGCGGCTCCGCGACCGCCGGCTCAGCACGCCGGAGCTGCCCTTCGACCTCAACGGC
GGCTACGTCGGCTACTTCGGCTACGAGCTCAAGGGCGAGTGCGGCAGCCCGAACGTCCAC
CGGTCCGAAACCCCGGACGCCGTCTGGACGTTCGCCGACCGGCTGATCGCGGTGGACCAC
CTGGAAGAGAAGACCTACGTCGTGGCCGTGAGCGGCACCGGGCCGGGCGAGGAGGACGCG
GCCCGGGCCTGGGTGACGGAGACCGCCCGGCGGGCCCGCGAACTGCCGGACCCCGCCCCG
TCCGAGGCACCCGCCCCGGCCGCCACGGCGCCGTCCGAACAGGTCGACATCGCCCGCGAC
CGCGGCCAGTACGTGAGCGACATCAAGGACTGCCTCGACGAGCTGCACAGCGGCGAGAGC
TACGAGATCTGCCTGACCAACCGGATCCGGCTGCCCGAGCTGAAGGACCCGCTCGGCTAC
CACCTCCTGCTCCGGCGCCTCAACCCCGCCCCCTACGGCGCGTTCCTGCGCCACGAGGGC
GTGAGCGTCGTGTGCTCCTCCCCGGAGCGGTTCCTGCGGATCGACCGGGACGGCGTCGTG
GAGAGCCGGCCCATCAAGGGAACGGCCCCCCGGGGCGAGGACCCGGCCGCCGACGAGGCG
CTCCGGGTGTCGCTCGCCGCCTCGGCCAAGACCCGGGCCGAGAACCTCATGATCGTCGAC
CTGCTCCGCAACGACCTGGGCCGGGTGTGCGAGGTCGGTTCCGTCGACGTCCCCGCCTAC
ATGGTCACCGAGACGTACGCGACGGTGCACCAGCTGGTCACCACGGTCCGGGGCCGCCTG
CGGCACGACGTGGACCCGGTCGCCTGCGTCCGGGCCTGCTTCCCCGGCGGCTCGATGACG
GGCGCCCCGAAGCTGCGGACGATGGCCATCATCGACCGCCTGGAGCAGGAGGCCCGCGGC
ATCTACTCCGGCACCATCGGCTACTTCGGGCTCTCCGGCGCCGCGGACCTCAACATCGTC
ATCCGCACCGCCGTCTCCACCGGTACGGGCACGGTCATCGGCACCGGCGGCGCCATCGTC
CTCGACTCGGACCCGGACGAGGAGTTCGACGAGGTGCTGCTCAAGGGCCACGCGCTGGTG
CGGGCGCACGGGCTGCTGGAGGGGGCCGCGGAGGCCCCGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005801 ADC synthase (Domain)
[192-673] 1.7e-122 SSF56322
SSF56322   ADC synthase
[200-664] 1.5e-139 G3DSA:3.60.120.10
G3DSA:3.60.120.10   no description
IPR005802 Para-aminobenzoate synthase, component I (Family)
[303-657] 5.5e-105 TIGR00553
TIGR00553   pabB: aminodeoxychorismate synthase, component I
IPR006221 Anthranilate synthase/para-aminobenzoate synthase, glutamine amidotransferase (Family)
[10-166] 3.1e-36 TIGR00566
TIGR00566   trpG_papA: glutamine amidotransferase of anthrani
IPR006805 Anthranilate synthase component I, N-terminal (Domain)
[208-347] 8.3e-21 PF04715
PF04715   Anth_synt_I_N
IPR015890 Chorismate binding, C-terminal (Domain)
[401-655] 1.9e-84 PF00425
PF00425   Chorismate_bind
IPR017926 Glutamine amidotransferase type 1 (Domain)
[23-164] 4.2e-35 PF00117
PF00117   GATase
[1-174] 22.336 PS51273
PS51273   GATASE_TYPE_1
SignalP No significant hit
TMHMM No significant hit
Aureo_00040 Aureo_00040   Aureo_00060 Aureo_00060
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