Nidda_00010 : CDS information

Location

Organism	Streptomyces caelestis
Strain	NRRL 2821
Entry name	Niddamycin
Contig	AF016585
Start / Stop / Direction	396 / 1 / - [in whole cluster] 396 / 1 / - [in contig]
Location	complement(1..396) [in whole cluster] complement(1..396) [in contig]
Type	CDS
Length	396 bp (131 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450(fragment)
Product (GenBank)	cytochrome P-450 hydroxylase homolog
Gene
Gene (GenBank)	nidi
EC number
Keyword
Note
Note (GenBank)	ORF6; similar to tyli
Reference	ACC O30763 PmId [9393718] Identification and characterization of the niddamycin polyketide synthase genes from Streptomyces caelestis. (J Bacteriol. , 1997) comment ORF6: P450(N-terminal portion) ORF6はmacrolide tylosin生合成経路においてcytochrome P450 hydroxylaseをコードするtylIにN末AAの相同性がある。tylIもORF6と同じようにPKSに隣接して逆向きに並んでいる。
Related Reference	ACC P48635 PmId [8416893] Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. (J Bacteriol. , 1993) [7849045] ( , ) comment 44th(P48635) 41%, 2e-11, N末hit Saccharopolyspora erythraea_eryK Erythromycin C-12 hydroxylase(EC 1.14.13.154) --- [PMID: 8416893](1993) Saccharopolyspora erythraea (Streptomyces erythraeus)において、P450 monooxygenase familyに属するEryKが、erythromycin生合成の最終反応の１つであるmacrolactone ringのC-12 hydroxylationに関与していることをeryK変異体を作成して調べている。 --- [PMID: 7849045](1995) 不溶性封入体としてE.coliでEryKを過剰産生し、可溶化、再折りたたみ、active holo-EryK再構築をした。動態確認で、erythromycin Bより1200-1900倍もerythromycin Dを基質として好むことが分かった。

Sequence

>putative cytochrome P450(fragment) [cytochrome P-450 hydroxylase homolog]
MVDSVTGPMELSKDANAKELLDWFSHNRTHHPVFWDEGRQAWQVFRYDDYLTVSNHPEFF
SSDFTEVAPTPPELEMILGPGTIGALDPPAHGPMRKLVSQAFTPRRMAGQEQRIRVIAEE
LLDRVRGQKTIA

>putative cytochrome P450(fragment) [cytochrome P-450 hydroxylase homolog]
ATGGTCGACAGCGTGACTGGGCCGATGGAGCTCAGCAAGGACGCGAATGCCAAGGAGCTG
CTCGACTGGTTCAGCCATAACCGGACCCACCACCCCGTCTTCTGGGACGAGGGCCGTCAA
GCGTGGCAGGTGTTCCGGTATGACGACTACCTGACCGTCTCGAATCATCCCGAGTTTTTC
TCGTCGGATTTCACGGAGGTCGCGCCGACCCCGCCCGAGCTGGAGATGATCCTCGGGCCC
GGCACCATCGGCGCCCTGGACCCGCCCGCCCACGGCCCCATGCGCAAGCTGGTCAGCCAG
GCGTTCACACCCCGGCGGATGGCCGGTCAGGAACAGCGGATCCGGGTCATCGCCGAGGAA
CTGCTGGACCGGGTGCGTGGGCAGAAGACCATCGCG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

first Nidda_00020