Stigm_00150 : CDS information

Location

Organism	Stigmatella aurantiaca
Strain	Sga15
Entry name	Stigmatellin
Contig	AJ421825
Start / Stop / Direction	50,205 / 54,401 / + [in whole cluster] 50,205 / 54,401 / + [in contig]
Location	50205..54401 [in whole cluster] 50205..54401 [in contig]
Type	CDS
Length	4,197 bp (1,398 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	StiG protein
Gene
Gene (GenBank)	stiG
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q8RJY0 PmId [11809757] The biosynthesis of the aromatic myxobacterial electron transport inhibitor stigmatellin is directed by a novel type of modular polyketide synthase. (J Biol Chem. , 2002) comment S. aurantiacaにおいてstigmatellin形成を担う遺伝子セットの報告。 stiG (4197 bp) KS-(37-463), AT-(580-898), DH-(908-1125), ACP-(1258-1358) KR domainがないのにDH domainがあり、このDH domainはStiBで足りない分を補完するかもしれない。 [13C]acetate and [13C]propionate取り込み実験で、polyketideを合成する基質が確認されている。

PKS/NRPS Module

methylmalonyl-CoA

KS	37..413

AT	577..890

DH	945..1114

ACP	1275..1345

Sequence

>polyketide synthase [StiG protein]
MSKPANPVEQLSPLQQALLALREMRAKLDASERARTEPIAVVGMGCRVPGGASNAELLWA
LLRDGVDAISEVPADRWDLKDYYDPDPDAVGKMYTRHGGFVDAIDRFDAQFFGIAPKEAS
RMDPQQRLLLEVTWEALEHAGIAVDRLTGSSTGVFVGLCNNDYSFLHMRSGAGDAPDTYS
ATGSALSIASGRISYLLGLQGPCMTVDTACSASLLTVHLACQSLRQRECNLALAGGVNAI
LLPEGTVSGCQLRALAPDGRCKTFDAAANGYVRSEGCGMVVLKRLSDALADGDDILAVIR
GSAVNQDGRSGGLTAPNGPAQEDVIRKALARAGVEPKQVGYVEAHGTGTSLGDPIEVRAL
GAVLGAGRSKEQPLLLGSIKTNVGHLEGAAGIAGLLKVVLMLKHGEIPPHLHLSRPNPYI
SWDELPLAIPARRIPWAPIDGRRIAGVSSFGLSGTNVHVVLEAAPPRTAATSGPAAPRSE
SDLFHLLPLSARTKGALESLAKEYLGLLSQEAPAAAPLLQDVCRSASVRRSHHEHRLAVV
GKTHAELSEQLQNFLRGETLAGLSTGYTVPGERRKVVFIFPGQGSQWVGMGRKLLAQEPV
FRAAIERCDEEMRTHLNGSLLEALRDGSAALPLDRIDVLQPALFAFEVALAALWRSWGIE
PDAVIGHSMGEVAAAHVAGILSLPDAVRIICQRSKLLRRVSGQGAMAVVALSMEAVRREL
AGYEDRLAVAVSNSPASTVISGDPTVLRELLGRFKERDIFCREVKVDVASHSPQMDPLRG
ELLQALTGISARPASVPFYSTVKDRLVEGSELDVAYWADNLREPVLFGNAVQGLLSEGHE
IFLELSPHPILLTPVEEILHASGRQGAALPSVRRDAPEQATLLASLGSLYSLGASVDWMK
LATDGQFVRLPSYPWQRQRFWHPAAELAPRARVERGGRQESEQGHPLLGGYLPLARPEGT
HAWEIVLDPEAQPFLFEHRIKGAALLPGSVFLEMALAAAKEALGWQPLVVSEMELHRALV
LPKHGTCAVQLLIEPVREGRAGFQIYGRASASESWSLHVTGTLREKHGELESLTKEELAL
SAIEARCDTEVPGERFYERIASMGVQLGSSQQLIEHLRQREGEALASLRIPDGLVSELST
YVLHPSLVDACFQLFGAMSMASGTQESGDFVHLPVHLGGVQVVRRPEGRLWAHARLDLEA
SDGELPSGSVRLLDETGKTLVLMKGVRFMRVHREALEAQASPDAASSAPGQEAPGRARIA
HLVAGAPAARRRGLLLAEVCSDVCGVLGFDPAAPLETRLGFFKMGMDSLTAMKLRKRLEV
GIGKSLPPTLVFEYPNIEQLTDFLGRELAIHSAAPENTPVGNPSRGIPDELEGLPEEELI
ALFDREMAALERTSGEKS

>polyketide synthase [StiG protein]
ATGAGTAAACCGGCCAATCCCGTCGAGCAGTTGTCTCCGTTGCAGCAAGCGCTGCTCGCG
CTCCGTGAAATGCGCGCGAAGCTCGATGCTTCGGAGCGCGCTCGCACCGAGCCCATCGCC
GTCGTCGGGATGGGATGCCGCGTCCCTGGCGGCGCGAGCAATGCAGAGCTGCTCTGGGCA
TTGCTCCGCGATGGTGTGGACGCGATCTCCGAAGTCCCGGCTGATCGCTGGGACCTCAAG
GATTACTACGACCCGGATCCCGACGCCGTGGGCAAGATGTACACGCGTCACGGAGGGTTC
GTTGACGCCATCGATCGCTTCGATGCGCAGTTCTTCGGGATTGCTCCGAAGGAAGCCAGC
CGGATGGACCCGCAGCAGCGTCTGCTGCTCGAGGTGACGTGGGAGGCGCTGGAGCACGCG
GGGATCGCTGTCGATCGGCTCACGGGCTCGTCGACCGGCGTCTTCGTGGGGCTTTGCAAC
AACGACTATTCCTTCCTCCATATGCGCTCGGGCGCTGGCGACGCGCCCGATACGTATTCG
GCCACTGGAAGTGCCCTCAGCATTGCCTCGGGACGCATCTCGTACCTGCTCGGTCTGCAA
GGCCCTTGCATGACCGTGGACACCGCGTGTTCCGCGTCACTTTTGACCGTTCACCTTGCG
TGTCAGAGCCTGCGCCAGCGCGAGTGCAACCTCGCCTTGGCTGGCGGCGTCAATGCCATC
CTTCTCCCAGAAGGCACGGTCAGTGGTTGCCAGCTTCGGGCCCTCGCGCCTGATGGGCGG
TGCAAGACGTTCGACGCCGCGGCGAACGGCTATGTGCGGAGTGAAGGGTGCGGAATGGTG
GTGCTCAAGCGCCTTTCCGACGCCCTCGCGGATGGGGATGACATCCTCGCCGTCATCCGG
GGCTCCGCCGTCAATCAAGACGGACGCAGCGGTGGGCTCACGGCACCCAATGGTCCGGCA
CAGGAAGATGTGATTCGCAAGGCTCTGGCTCGGGCTGGCGTCGAGCCCAAGCAGGTGGGC
TATGTCGAGGCTCACGGGACGGGCACTTCGCTCGGCGATCCGATCGAAGTCCGTGCGCTG
GGCGCGGTTCTCGGGGCGGGGCGCTCAAAAGAGCAGCCGCTGTTGCTGGGTTCGATCAAA
ACCAACGTGGGGCACTTGGAGGGGGCGGCGGGCATCGCGGGTCTGCTCAAAGTGGTGTTG
ATGCTGAAGCACGGCGAGATCCCGCCCCACCTGCACCTGTCACGGCCGAACCCCTATATC
TCCTGGGACGAACTCCCCCTGGCCATTCCGGCCAGGCGGATTCCCTGGGCGCCCATCGAC
GGCCGACGAATCGCCGGCGTGAGTTCGTTCGGGCTCAGCGGGACCAATGTTCACGTCGTG
CTCGAAGCAGCTCCGCCGCGCACGGCGGCCACTTCGGGCCCTGCCGCGCCTCGTTCTGAG
TCGGACTTGTTCCACTTGTTGCCGCTGTCCGCGCGGACGAAAGGCGCACTCGAGTCCCTC
GCCAAGGAATATCTCGGGCTGCTCTCTCAGGAAGCTCCTGCAGCCGCCCCGCTGCTTCAG
GACGTCTGCCGTTCCGCCAGCGTCCGGCGCAGCCACCACGAGCATCGGTTGGCTGTTGTG
GGAAAAACGCACGCGGAGCTCTCCGAACAGCTCCAAAACTTCCTCCGGGGAGAGACTCTG
GCTGGGCTTTCGACCGGATACACGGTTCCCGGCGAGCGGCGGAAGGTCGTTTTCATCTTC
CCCGGACAAGGTTCGCAGTGGGTTGGCATGGGACGAAAGCTCCTCGCGCAGGAGCCCGTG
TTCCGTGCCGCCATTGAACGGTGCGACGAGGAGATGCGGACGCACCTCAACGGTTCGCTT
CTGGAGGCGTTGAGGGACGGTTCGGCTGCCCTGCCGCTGGATCGAATCGATGTCCTCCAG
CCCGCTTTGTTCGCGTTCGAAGTCGCCCTCGCCGCGCTTTGGCGCTCCTGGGGCATCGAG
CCGGATGCCGTCATCGGTCACAGCATGGGAGAGGTGGCCGCAGCACACGTCGCGGGCATC
CTGAGCCTTCCGGACGCGGTGCGGATCATCTGCCAGCGCAGCAAGCTCCTGAGAAGGGTG
AGCGGGCAGGGGGCCATGGCGGTCGTGGCGCTGTCGATGGAAGCCGTTCGCCGGGAGCTT
GCCGGGTATGAGGACCGGTTGGCCGTGGCCGTCAGCAACAGTCCTGCCTCCACCGTCATC
TCGGGAGATCCGACGGTGCTTCGCGAGCTTCTGGGTCGCTTCAAGGAACGCGACATCTTC
TGCCGTGAGGTGAAGGTCGACGTTGCGTCGCACAGCCCCCAGATGGATCCCCTCCGGGGG
GAGTTGCTCCAAGCCCTGACCGGAATCTCCGCCCGCCCCGCGTCCGTGCCCTTCTACTCG
ACCGTCAAGGATCGGCTCGTGGAAGGCTCCGAACTCGACGTGGCCTACTGGGCAGACAAC
CTGCGCGAGCCCGTGCTTTTTGGCAACGCGGTGCAGGGGTTGCTTTCCGAAGGGCACGAG
ATTTTCCTGGAGCTCAGCCCGCATCCCATTCTGCTCACGCCCGTGGAGGAGATTCTTCAC
GCCTCGGGGCGGCAGGGGGCGGCGCTTCCTTCCGTCCGGCGCGATGCACCCGAGCAGGCG
ACGTTGCTCGCGTCTCTGGGGTCGCTGTACTCGCTCGGTGCGTCCGTGGACTGGATGAAG
CTGGCCACGGACGGCCAATTCGTCCGGTTGCCCTCGTATCCCTGGCAGCGGCAGCGCTTC
TGGCATCCCGCGGCGGAATTGGCTCCACGCGCTCGCGTGGAGCGGGGGGGGCGCCAGGAG
TCGGAGCAGGGTCACCCCCTTCTCGGTGGGTATCTGCCCCTGGCTCGGCCTGAGGGCACC
CATGCCTGGGAGATCGTTCTCGACCCCGAGGCTCAGCCTTTCCTCTTCGAGCACCGGATC
AAGGGGGCCGCGCTGCTCCCTGGCTCGGTGTTCCTGGAGATGGCGCTTGCCGCGGCAAAA
GAGGCGCTGGGTTGGCAACCCCTGGTGGTTTCGGAGATGGAGCTCCATCGCGCACTCGTC
CTGCCGAAGCACGGCACGTGCGCTGTCCAGCTCTTGATCGAGCCGGTGCGAGAAGGGCGT
GCGGGTTTCCAGATCTATGGCCGCGCATCCGCGTCCGAATCGTGGAGCTTGCACGTGACC
GGCACGCTCCGGGAAAAGCACGGGGAACTCGAGTCCCTCACGAAGGAAGAACTCGCGCTG
AGCGCGATCGAAGCGCGATGCGACACCGAGGTGCCGGGCGAGCGCTTCTACGAGCGAATT
GCTTCCATGGGGGTCCAGCTCGGTTCCTCGCAGCAACTCATCGAGCACCTCCGTCAGCGT
GAGGGGGAGGCACTGGCATCCTTGCGCATTCCGGATGGGCTCGTGTCGGAACTCTCCACC
TACGTGCTCCACCCGAGCCTCGTCGATGCCTGCTTCCAGCTCTTTGGCGCGATGTCGATG
GCGTCAGGCACGCAGGAGTCAGGGGACTTCGTCCATCTGCCTGTCCACCTGGGAGGAGTG
CAGGTCGTGCGGCGTCCCGAAGGCCGACTTTGGGCTCATGCCCGTTTGGATCTCGAAGCG
TCGGATGGTGAGCTCCCCTCGGGCAGCGTCCGCCTGCTCGATGAGACTGGCAAGACGCTG
GTGTTGATGAAGGGCGTTCGGTTCATGCGCGTCCACCGGGAGGCGCTTGAGGCCCAGGCT
TCGCCGGATGCTGCTTCGTCCGCGCCGGGTCAGGAAGCGCCTGGACGTGCTCGGATCGCC
CACCTTGTCGCAGGGGCTCCAGCGGCACGGCGTCGCGGCCTGTTGCTCGCAGAGGTCTGC
TCCGATGTCTGCGGTGTGCTCGGCTTCGATCCGGCGGCTCCCTTGGAGACGCGCCTCGGC
TTCTTCAAGATGGGCATGGATTCGCTGACGGCGATGAAGCTCCGGAAGCGCCTCGAAGTG
GGCATTGGAAAGTCGCTGCCTCCCACCCTGGTTTTCGAATATCCCAACATCGAGCAGCTC
ACGGACTTCCTCGGGCGAGAACTCGCCATCCACTCCGCGGCGCCCGAGAACACGCCGGTG
GGGAACCCTTCCCGTGGCATCCCGGATGAACTGGAAGGGCTTCCGGAAGAGGAGCTCATC
GCGCTGTTCGATCGCGAAATGGCCGCCCTCGAGCGGACCTCTGGAGAGAAGTCATGA

[7] KS	37..413

[7] AT	577..890

[7] methylmalonyl-CoA	768..772

[7] DH	945..1114

[7] ACP	1275..1345

[7] KS	109..1239

[7] AT	1729..2670

[7] methylmalonyl-CoA	2302..2316

[7] DH	2833..3342

[7] ACP	3823..4035

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) PS50075 ACP_DOMAIN G3DSA:1.10.1200.10 ACP_like PF00550 PP-binding SSF47336 ACP_like IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR018201 Beta-ketoacyl synthase, active site (Active_site) PS00606 B_KETOACYL_SYNTHASE IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 PKS_PP IPR020807 Polyketide synthase, dehydratase domain (Domain) SM00826 PKS_DH IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 PKS_KS
SignalP	No significant hit
TMHMM	No significant hit

Stigm_00140 Stigm_00160