Stigm_00180 : CDS information

Location

Organism	Stigmatella aurantiaca
Strain	Sga15
Entry name	Stigmatellin
Contig	AJ421825
Start / Stop / Direction	63,070 / 63,840 / + [in whole cluster] 63,070 / 63,840 / + [in contig]
Location	63070..63840 [in whole cluster] 63070..63840 [in contig]
Type	CDS
Length	771 bp (256 aa)

Annotation

Category	3.2 modification methylation
Product	putative methyltransferase
Product (GenBank)	methyl transferase
Gene
Gene (GenBank)	stiK
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q8RJX7 PmId [11809757] The biosynthesis of the aromatic myxobacterial electron transport inhibitor stigmatellin is directed by a novel type of modular polyketide synthase. (J Biol Chem. , 2002) comment S. aurantiacaにおいてstigmatellin形成を担う遺伝子セットの報告。 stiK (771 bp) Similar to methyltransferases 本ORFはStigmatellinの骨格が出来た後で芳香環部分のHydroxy基にMethyl基を転移させ、OMeとすると推測される。
Family/Domain	FD IPR010233, IPR013216 comment [IPR010233] Ubiquinone biosynthesis O-methyltransferase (Family) function; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity [IPR013216] Methyltransferase type 11 (Domain) function; methyltransferase activity

Sequence

>putative methyltransferase [methyl transferase]
MPVNNGMYDEMNWWNDDSSVSLLSTSINPCRIAYFHNLLTNRIGLDPTKVRVLDVGCGGG
LLAEEFSKKGWKVTGVDQSAPSIELAKAHSAKVGLNIDYHVSKAQKLPFEDETFDIVYCC
DVLEHIPTYPAAVAESARVLKKGGVYLYETINRTFMSKMIAIKMLQEWSWTRVIPDDVHD
WKMFIRPEELHNAMHAAKIQNVETAGMTVDNPVGFIRAILRHRRGHSSLGDVGRVAPAVV
SKDLSMGYMGFGVKTA

>putative methyltransferase [methyl transferase]
ATGCCTGTCAATAATGGGATGTATGATGAGATGAACTGGTGGAATGACGACTCGTCCGTC
AGCCTTCTGTCGACGTCGATCAACCCGTGCCGCATTGCCTATTTCCACAACCTGCTCACC
AACCGGATTGGGCTCGATCCCACGAAGGTGCGCGTGCTCGACGTCGGCTGTGGCGGCGGT
CTGCTGGCCGAGGAGTTTTCCAAGAAGGGCTGGAAAGTGACGGGCGTCGATCAGTCCGCC
CCGTCCATCGAGCTGGCCAAGGCCCACTCCGCGAAGGTGGGGCTCAACATCGACTACCAC
GTCTCCAAGGCTCAGAAGCTCCCGTTCGAGGACGAGACCTTCGACATCGTCTATTGCTGT
GATGTGCTCGAGCACATTCCCACCTATCCCGCCGCCGTCGCGGAGAGCGCTCGGGTGCTC
AAGAAGGGGGGCGTCTATCTCTACGAGACCATCAACCGGACGTTCATGAGCAAGATGATC
GCCATCAAGATGCTTCAGGAGTGGTCCTGGACGCGGGTGATCCCCGACGATGTGCACGAC
TGGAAGATGTTCATCCGTCCCGAGGAGCTTCACAACGCGATGCATGCAGCGAAGATCCAG
AACGTCGAGACGGCGGGCATGACGGTCGACAATCCCGTGGGCTTCATCCGGGCGATCCTC
CGCCACCGCCGGGGCCACTCCTCGCTCGGCGATGTGGGACGCGTCGCGCCCGCGGTGGTC
TCGAAGGATCTGTCGATGGGGTACATGGGGTTCGGGGTCAAGACCGCCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR010233 Ubiquinone biosynthesis O-methyltransferase (Family) TIGR01983 UbiG IPR013216 Methyltransferase type 11 (Domain) PF08241 Methyltransf_11
SignalP	No significant hit
TMHMM	No significant hit

Stigm_00170 Stigm_00190