first first   Aver_00020 Aver_00020

Aver_00010 : CDS information

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Organism
StrainATCC 31267 (=NBRC 14893)
Entry nameAvermectin
Contig
Start / Stop / Direction327 / 3,176 / + [in whole cluster]
327 / 3,176 / + [in contig]
Location327..3176 [in whole cluster]
327..3176 [in contig]
TypeCDS
Length2,850 bp (949 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
ProductLuxR family transcriptional regulator
Product (GenBank)
Gene
Gene (GenBank)aveR
EC number
Keyword
Note
Note (GenBank)
  • contains helix-turn-helix DNA binding domain; positive response regulator
Reference
ACC
PmId
[10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999)
[19148632] Characterization of a regulatory gene, aveR, for the biosynthesis of avermectin in Streptomyces avermitilis. (Appl Microbiol Biotechnol. , 2009)
[20012992] The pathway-specific regulator AveR from Streptomyces avermitilis positively regulates avermectin production while it negatively affects oligomycin biosynthesis. (Mol Genet Genomics. , 2010)
[26298701] The PhoP transcription factor negatively regulates avermectin biosynthesis in Streptomyces avermitilis. (Appl Microbiol Biotechnol. , 2015)
comment
[PMID:10449723](1999)
avermectin生合成gene clusterの報告。

AveR: positive regulator

aveR mutantsの表現型、DNA segmentsを導入することによるその相補特性、helix-turn-helix motifを持つタンパクへの配列類似から、AveRがpotential regulatory functionを持つことが示される。

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[PMID: 19148632](2009)abstract
aveR 不活化mutantは、avermectin中間体をいかなるavermectin派生体にも変換できない。よってAveRは、avermectinの生合成遺伝子と修飾遺伝子の両方の発現を調節するpositive regulatorであることが示唆された。
また、多量のaveRはavermectinの完全な損失をもたらすことから、aveRには最高許容限界濃度がある。

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[PMID: 20012992](2010)abstract
aveR deletion mutantはavermectin産生を消失し、oligomycin産生が増える。この表現型はaveR geneのsingle copyによって相補された。

AveRのC末HTH domainの除去はavermectin生合成を消失。
ave clusterとolm clusterの両方のpredicted promoter regionsがAveRのtarget sitesであり、AveRのDNA-binding activityはHTH domainに依存的である。
aveの構造遺伝子の転写はAveRに依存的であるが、olmの構造遺伝子とputative pathway-specific regulatory genesの転写はaveR mutantsで増える。

avermectin PKS_AVES1をコードするaveA1の転写がAveRによって活性化される。
aveRの過剰発現でavermectin産生増加。

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[PMID: 26298701](2015)abstract
aveR転写はPhoPによって抑制される。

close this sectionSequence

selected fasta
>LuxR family transcriptional regulator
MQGVSCLHPPRKPEELTLVDRETQFRALRLALTECAAGTVKLLVAEGGMGCGKSTFLGEA
LHTAAASGFAVLRAAGLPADHRQPLGVLQQLLNDPAPEDTARTAVRPMPVQHVRGALERL
AAGAPLAIGIDDVQDADPESLHCLMRLTRHSPTSRILLLCTALACSPAADPVLEAELMRQ
TAFERITLDCLSLDGVTGLVSDRCARPTAPPPADYCLTVTGGNPLLLRALLEEHSEADAP
SAPRPAEPSALHSPPQAAPPRPVVGGRFYQSVLACLSRTETAIRQTAGALAVLGGRARAD
LLPQLLGASPASVTRGLRALEATGLTTSGRFRHPVAEAAALDALDPSRRAHLHRRAAALQ
HHDGAAPRDVARHLLAARHAAGPWAVSVLRDAAEQSLAQDDVASAVSCLELAYGACVRER
ERPEIRIRLAAAFGRTNIAVAEEHLADLVATLREGELTGHQTALLVPLLVNHGRLGEARE
AMDRLNAADDARGLCADGGFPMAAPWPSTAHLAARRDPAARRDPGTRRDPADKPFLPRQS
GAPQPRPEDGRGQQPTAALWALPGNGTSEAAAHAAEQVLRSSPLTDSTLVLLVNAVRILA
RTGRYDTADIWCHRLLGEATRRRCPGWQAHLLAVRAELSLCRGLLADAKECAQRALTHVP
GHSRSVFAGGPLACQVLACTAMGRYDEATQLLSHPVPEALFHSVYGLGYLRARGHFHLAM
NRLPAAVRDFLTAGRVAREWGLDHPVLLPWRTDAAEAFLRLGETKRADQLLTEQLVSPHS
GNPYVRGTALRLRAQTAAPAERLRLLSEAVSDLQSSGDRLALARALADLGAAYHSRNEPV
RASATVRRAWQLAKECGAQALCDSILPSRGTKDRGPDGRAAATEALLSESEMRVATLAAG
GNTNREIAGRLCVTVSTVEQHLTRVYRKLNITRRRELPTRLRHLADQAN
selected fasta
>LuxR family transcriptional regulator
ATGCAGGGAGTTTCCTGTCTGCACCCCCCTCGGAAACCAGAAGAACTCACGCTCGTCGAC
CGGGAAACACAGTTCCGCGCGCTGCGGCTGGCCCTTACCGAATGCGCGGCCGGCACGGTG
AAACTGCTCGTCGCCGAGGGCGGAATGGGCTGTGGAAAGAGTACGTTCCTGGGCGAGGCA
CTGCACACCGCCGCCGCCTCCGGCTTCGCCGTCCTGCGTGCCGCCGGGCTTCCCGCGGAC
CACCGGCAACCCCTCGGCGTACTGCAGCAACTGCTGAACGACCCCGCCCCCGAGGACACC
GCCCGCACCGCCGTCCGCCCCATGCCGGTGCAACACGTCCGCGGCGCCCTCGAACGCCTC
GCCGCCGGCGCCCCGCTGGCGATCGGCATCGACGACGTACAGGACGCGGACCCGGAGTCG
CTGCACTGTCTGATGCGCCTCACCCGCCACTCCCCCACCTCGCGGATCCTGCTGCTGTGC
ACCGCCCTGGCGTGCAGTCCGGCCGCCGACCCGGTACTCGAAGCCGAGCTGATGCGTCAG
ACCGCCTTCGAACGCATCACGCTGGACTGCCTGTCCCTGGACGGAGTGACCGGGCTGGTC
TCGGACCGCTGCGCGCGGCCCACGGCGCCCCCGCCGGCGGACTACTGCCTGACCGTCACC
GGGGGCAACCCGCTGCTGCTGCGGGCCCTGCTCGAAGAGCACAGCGAGGCCGACGCCCCC
TCGGCACCCCGCCCGGCGGAGCCCTCCGCGCTGCACTCCCCGCCGCAGGCCGCCCCGCCG
CGCCCGGTCGTCGGCGGCCGCTTCTACCAGTCCGTACTGGCCTGCCTGTCCCGCACGGAG
ACGGCGATCAGGCAGACGGCCGGCGCCCTCGCCGTCCTCGGCGGGCGTGCGCGCGCCGAC
CTGCTCCCCCAACTGCTCGGCGCGAGTCCCGCGTCGGTCACCCGGGGGCTGCGTGCGCTG
GAGGCGACGGGGCTGACCACCTCCGGCCGTTTCCGGCACCCGGTGGCCGAGGCCGCCGCG
CTCGACGCACTGGACCCGAGCCGCCGTGCCCACCTGCACCGCCGAGCGGCGGCGCTGCAG
CACCACGACGGCGCGGCGCCGCGGGACGTCGCCCGCCACCTTCTCGCGGCCCGCCATGCG
GCGGGCCCCTGGGCGGTGTCCGTGCTGCGTGACGCCGCCGAGCAGTCGCTGGCGCAGGAC
GACGTGGCGTCGGCGGTCTCCTGCCTGGAACTCGCCTACGGGGCCTGTGTCCGGGAACGG
GAACGTCCGGAGATCAGGATCAGGCTCGCCGCCGCTTTCGGGCGCACCAACATCGCGGTG
GCGGAAGAGCACCTCGCCGACCTGGTCGCCACCTTGCGGGAAGGAGAATTGACCGGCCAT
CAGACGGCTTTACTCGTCCCTCTGCTCGTCAACCACGGCCGCCTCGGCGAAGCGCGGGAG
GCGATGGACCGGCTCAACGCCGCCGACGACGCGCGCGGCCTGTGCGCGGACGGCGGCTTC
CCGATGGCCGCTCCGTGGCCGTCGACCGCACACCTCGCCGCACGCCGCGATCCCGCCGCG
CGCCGCGATCCCGGCACCCGCCGCGATCCCGCCGACAAGCCGTTCCTGCCCCGGCAGTCC
GGTGCCCCGCAGCCCCGGCCGGAGGACGGCCGTGGCCAGCAGCCGACAGCGGCCTTGTGG
GCCCTGCCCGGAAACGGCACCAGCGAGGCGGCCGCACACGCCGCGGAACAGGTACTGCGG
TCCTCCCCGCTCACCGACAGCACCCTCGTGCTCCTGGTGAACGCCGTGCGGATCCTCGCC
CGCACGGGCCGGTACGACACCGCGGACATCTGGTGCCACCGCCTGCTCGGCGAGGCCACC
CGTCGCCGTTGTCCCGGCTGGCAGGCGCACCTCCTGGCGGTGCGGGCCGAACTCTCGCTG
TGCCGTGGCCTGCTCGCCGACGCCAAGGAGTGCGCCCAGCGCGCACTGACACACGTACCG
GGGCACAGCCGCAGCGTCTTCGCGGGCGGTCCGCTGGCCTGCCAGGTCCTCGCCTGCACC
GCGATGGGACGCTACGACGAAGCCACGCAACTGCTCAGCCATCCGGTTCCCGAGGCGCTG
TTCCACAGTGTGTACGGCCTGGGGTACCTGCGGGCCCGGGGCCATTTCCACCTGGCCATG
AACCGCCTGCCCGCCGCCGTCCGCGACTTCCTCACCGCCGGCCGGGTGGCGCGGGAGTGG
GGACTGGACCATCCGGTGCTGCTGCCCTGGCGTACGGACGCCGCGGAGGCGTTCCTCCGG
CTCGGGGAAACGAAGAGGGCCGACCAACTCCTCACCGAACAGCTCGTCTCCCCGCACAGC
GGCAACCCGTACGTCCGCGGCACCGCGCTGCGCCTGCGGGCCCAGACCGCGGCGCCGGCG
GAACGGCTCCGGCTGCTGAGCGAGGCGGTCAGTGACCTCCAGAGCTCCGGCGACCGCCTG
GCGCTGGCCCGCGCACTGGCCGATCTCGGCGCCGCGTATCACAGCCGGAACGAGCCCGTA
CGGGCGAGCGCCACGGTCCGCCGCGCCTGGCAGCTGGCCAAGGAGTGCGGAGCCCAGGCC
CTGTGCGACAGCATCCTGCCCAGTCGCGGCACCAAGGACCGGGGGCCCGACGGAAGGGCG
GCCGCGACCGAGGCCCTGCTGAGCGAGTCCGAGATGCGAGTCGCGACACTGGCGGCGGGC
GGCAACACCAACCGTGAGATCGCCGGCCGGCTCTGCGTCACCGTCAGCACGGTCGAACAG
CATCTGACGCGGGTCTACCGCAAACTGAACATCACCCGCCGCAGGGAGCTGCCGACCCGT
CTGCGACACCTCGCGGACCAGGCCAACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[884-941] 2.49999811956465e-19 SM00421
SM00421   HTH_LUXR
[887-901] 8.00000024242803e-08 PR00038 [901-917] 8.00000024242803e-08 PR00038 [917-929] 8.00000024242803e-08 PR00038
PR00038   HTHLUXR
[901-928] PS00622
PS00622   HTH_LUXR_1
[880-945] PS50043
PS50043   HTH_LUXR_2
[886-937] 4.6e-11 PF00196
PF00196   GerE
IPR011990 Tetratricopeptide-like helical (Domain)
[564-896] 4.40000000000001e-19 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[897-940] 2.1e-15 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[865-944] 3.7999986264479e-15 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
first first   Aver_00020 Aver_00020
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