Resis_00160 : CDS information

Location

Organism	Streptomyces resistomycificus
Strain	HKI-237
Entry name	Resistomycin
Contig	AJ585192
Start / Stop / Direction	13,522 / 12,323 / - [in whole cluster] 13,522 / 12,323 / - [in contig]
Location	complement(12323..13522) [in whole cluster] complement(12323..13522) [in contig]
Type	CDS
Length	1,200 bp (399 aa)

Annotation

Category	3.1 modification hydroxylation
Product	resistomycin hydroxylase
Product (GenBank)	RemO protein
Gene
Gene (GenBank)	remO
EC number
Keyword	resistoflavin
Note
Note (GenBank)
Reference	ACC Q70DW2 PmId [14982421] A gene cluster encoding resistomycin biosynthesis in Streptomyces resistomycificus; exploring polyketide cyclization beyond linear and angucyclic patterns. (J Am Chem Soc. , 2004) [17090047] The boat-shaped polyketide resistoflavin results from re-facial central hydroxylation of the discoid metabolite resistomycin. (J Am Chem Soc. , 2006) comment [PMID: 14982421](2004) resistomycin生合成gene cluster全体を異種性発現により同定。 RemO: Oxygenase 配列解析に基づき、resistomycin → resistoflavinへのhydroxylationを触媒するFAD-dependent oxygenaseかもしれないと述べている。 --- [PMID: 17090047](2006) resistomycin→resistoflavinへの珍しいenantioface-differentiating hydroxylationが、FAD-dependent monooxygenase_RemOによって触媒されるということをvivoとvitroの実験で実証している。

Sequence

>resistomycin hydroxylase [RemO protein]
MKTETAQCVVVGGGPAGMMAGLLLARQGVEVTVVEKHGDFLRDFRGDTVHPSTLQVIDEL
GLLEEFLQIPHTKVTDITVSTAAGPTTFADFSKIRSRYPYIAFMPQWDVLDFLARKAGEL
PGFRLLQKTEGTTVIREGDRVCGVRADTPEGPLEIRSDLVIAADGRHSTMRKAVGLRVAA
SAAPMDVLWFRLEREPGETMTFLHTGQGFVLITIDRGAYWQVAYVIPQGQYDAVRAEGLG
RLRADVSSVHPPFAERVEREIRDWDDVKLLGVRVDRLRTWYRPGLLCVGDAAHAMSPAGG
VGINLAVQDAVAAARMLGPVLRTGRTPTPQELRRVQRRRELPVRAVQLAQVHMLADLYPK
DRGSVVERPFAARLVRRFPSLNRWTAQFIGVGLRPEHVS

>resistomycin hydroxylase [RemO protein]
ATGAAGACCGAGACCGCCCAGTGCGTCGTCGTCGGCGGCGGCCCCGCCGGGATGATGGCA
GGACTGCTGCTCGCCCGGCAGGGCGTGGAGGTCACCGTCGTCGAGAAGCACGGCGACTTC
CTGCGGGACTTCCGCGGCGACACCGTGCACCCCTCGACCCTCCAGGTCATCGACGAACTG
GGGCTGTTGGAGGAGTTCCTGCAGATCCCGCACACCAAGGTCACCGACATCACCGTGAGC
ACGGCCGCGGGGCCGACCACGTTCGCCGACTTCTCCAAGATCCGCAGCCGCTACCCGTAC
ATCGCGTTCATGCCGCAGTGGGACGTCCTGGACTTCCTGGCCCGCAAGGCCGGGGAACTG
CCCGGCTTCCGGCTGCTGCAGAAGACCGAGGGCACCACCGTGATCCGCGAGGGCGACCGG
GTGTGCGGAGTGCGCGCGGACACCCCGGAGGGGCCGCTGGAGATCCGGTCCGACCTGGTG
ATCGCCGCGGACGGCCGCCACTCGACCATGCGCAAGGCCGTAGGACTGCGGGTGGCGGCG
AGCGCGGCGCCCATGGACGTGCTGTGGTTCCGGCTCGAGCGCGAACCCGGCGAGACCATG
ACCTTCCTGCACACCGGACAGGGCTTCGTGCTGATCACCATCGACCGGGGCGCCTACTGG
CAGGTCGCGTACGTCATCCCGCAGGGCCAGTACGACGCCGTACGGGCGGAGGGCCTCGGC
CGCCTCAGGGCCGACGTGTCCTCGGTGCACCCGCCGTTCGCCGAGCGCGTCGAGCGGGAG
ATCCGGGACTGGGACGACGTGAAGCTCCTCGGCGTCCGGGTCGACCGGTTGCGCACCTGG
TATCGGCCCGGCCTGCTGTGCGTCGGAGACGCGGCGCACGCCATGTCCCCGGCCGGCGGA
GTCGGCATCAACCTCGCCGTGCAGGACGCGGTGGCGGCCGCGCGGATGCTCGGGCCGGTG
CTGCGCACCGGACGTACGCCGACCCCGCAGGAGCTGCGCCGGGTCCAGCGGCGGCGCGAA
CTGCCGGTACGGGCCGTGCAGTTGGCGCAGGTGCACATGCTGGCCGACCTGTATCCGAAG
GACCGCGGGTCGGTGGTGGAGCGGCCGTTCGCGGCCCGGCTGGTGCGGCGCTTCCCGTCC
CTGAACCGGTGGACGGCGCAGTTCATCGGTGTGGGGCTGCGTCCTGAGCACGTGTCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002938 Monooxygenase, FAD-binding (Domain) PF01494 FAD_binding_3 IPR003042 Aromatic-ring hydroxylase-like (Domain) PR00420 RNGMNOXGNASE
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Resis_00150 Resis_00170