Aran_00020 Aran_00020   Aran_00040 Aran_00040

Aran_00030 : CDS information

close this sectionLocation

Organism
StrainTü303
Entry nameAranciamycin
Contig
Start / Stop / Direction3,617 / 5,569 / + [in whole cluster]
3,617 / 5,569 / + [in contig]
Location3617..5569 [in whole cluster]
3617..5569 [in contig]
TypeCDS
Length1,953 bp (650 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase
Product (GenBank)oxidoreductase
Gene
Gene (GenBank)
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in aranciamycin biosynthesis.
Note (GenBank)
  • orf3
Reference
ACC
PmId
[17357167] Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. (Chembiochem. , 2007)
comment
aranciamycin生合成gene cluster(ara-cluster)のクローニングとシークエンス報告。
ara clusterの5'末はORF4だという記述がある（理由は不明）。

ORF3(650aa): oxidoreductase
Related Reference
ACC
P07788
PmId
[3135411] Identification of the promoter for a spore coat protein gene in Bacillus subtilis and studies on the regulation of its induction at a late stage of sporulation. (J Mol Biol. , 1988)
[11514528] CotA of Bacillus subtilis is a copper-dependent laccase. (J Bacteriol. , 2001)
comment
Blast 37th, id32%, 6e-75
Bacillus subtilis_cotA
Spore coat protein A

--
[PMID: 3135411](1988)
cotAのPromoterと、転写開始点をsporulation(胞子形成)と germination (発芽)の色々なmutantを作成したり、調節領域付近のdeletion mutantを作成し調べている。
結局、胞子形成中期の様々な遺伝子の発現にcatAが絡んでいるのではないかと考察している。

--
[PMID: 11514528](2001)
B.subtilisのspore coat protein CotAは、manganese oxidasesやlaccasesといったmulticopper oxidasesに似ている。

B.subtilisはmanganeseを酸化できるが、CotAもその他のsporulation proteinsも関連しない。
laccasesの特異的な基質であるsyringaldazineは、wild-type sporesと反応するがdelta-cotA sporesとは反応しない。
よってCotAはlaccaseである。

CotAは、メラニン様でUV抵抗性のある茶色いspore pigmentの生合成に関与するかもしれない。

close this sectionSequence

selected fasta
>putative oxidoreductase [oxidoreductase]
MGSPVISRRLVLQTSLAGGMSLFCPPGTAMPRAAPAQAEVLDPAAIRKYVAELVVPPVMP
FDSRAGRDVYTIGIRQFRQQVLPPGMPATTVFGYGSTGHPETLHYPAFTIEAWYGRAVQV
RWVNQLLDPNGGHLPHILPVDPTLHWANPPGGAERRDSRPTFTSTPGPYGGPVPVVTHLH
GGANTEESDGYAEAWYLPDARGIPRGYARVGSFYQEFAKKSEAKFGVPWGPGEAVFHYAN
LSRAATMWFHDHTLGITRLNVYAGMSGLYLLRGGPSDLAEGVLPGPAPRLGDPPGKRYYE
IPVVIQDRSFRADGSLFYPASRALFDRFKGPYVPHSDIPPIWNPEYFGNAMVANGRTWPV
LPVEPRRYRLRFLNGCNARTLILKIVTDPMARRPVPAKLALWQIGSDGGFLPAPVRRDQL
LLAPAERADVVVDFSSTPVGSELYLINEGPDAVFAGGTVETGFPAANPKTTGQIMKLVIE
PLVTADTSTPPAQLTLPPVKPLGAADRTRQVSLGEVASARVRGVSPREVLLGVIGPNGEP
RLLGWADPITENPAVGSTEIWEIHNLTKDAHPIHIHEVQFQIVDRQKLPVGGRRPPEAWE
RGFKDTVLAYPQETTRVKVRFSQTGRYMWHCHLLEHEDNEMMRPLHVGPA
selected fasta
>putative oxidoreductase [oxidoreductase]
GTGGGAAGCCCAGTGATCAGCAGAAGGCTCGTGCTTCAGACCAGTCTGGCGGGCGGAATG
TCGCTCTTTTGCCCTCCCGGTACCGCTATGCCGCGGGCTGCGCCCGCGCAAGCGGAAGTC
CTTGATCCGGCGGCGATACGGAAGTACGTGGCCGAACTTGTGGTACCTCCCGTCATGCCG
TTCGACAGCCGGGCCGGCCGGGACGTCTACACGATCGGCATCCGGCAGTTCCGGCAGCAG
GTGCTTCCCCCGGGCATGCCGGCCACAACGGTGTTCGGATACGGTTCGACCGGGCATCCC
GAAACGCTCCACTATCCCGCGTTCACCATCGAAGCGTGGTACGGACGTGCGGTCCAGGTC
AGGTGGGTCAACCAACTCCTCGACCCGAACGGCGGACATCTGCCGCACATATTGCCGGTC
GATCCCACACTCCACTGGGCGAACCCACCGGGCGGGGCGGAGCGGCGGGACTCCCGTCCC
ACGTTCACTTCGACACCCGGGCCGTACGGCGGACCGGTACCCGTTGTCACCCACCTGCAC
GGCGGGGCGAACACGGAGGAGAGCGACGGATACGCCGAGGCCTGGTATTTGCCCGATGCG
CGGGGCATTCCCCGCGGTTACGCCCGAGTCGGGTCCTTCTACCAGGAGTTCGCGAAGAAG
TCCGAGGCGAAGTTCGGCGTGCCCTGGGGCCCGGGCGAGGCCGTCTTCCACTACGCCAAC
CTCAGCCGGGCCGCCACCATGTGGTTCCACGACCACACCCTGGGCATCACCCGGCTGAAC
GTGTACGCCGGAATGTCGGGCCTCTACCTGCTGCGCGGTGGGCCGTCGGATCTGGCGGAA
GGCGTCCTGCCCGGACCCGCGCCGAGGCTCGGTGACCCGCCCGGCAAGCGCTACTACGAA
ATTCCGGTCGTCATCCAGGACCGCTCGTTCCGGGCCGACGGGAGCCTGTTCTACCCCGCG
AGCCGGGCGCTCTTCGACCGGTTCAAGGGCCCCTATGTGCCCCACAGTGACATACCGCCG
ATCTGGAACCCGGAGTACTTCGGCAACGCGATGGTCGCCAACGGCAGAACCTGGCCGGTG
CTGCCCGTCGAGCCGCGCCGTTACCGACTCCGGTTCCTCAACGGCTGCAACGCACGCACT
CTGATCCTGAAGATCGTCACTGATCCGATGGCCCGCAGGCCGGTACCGGCAAAGCTTGCC
CTGTGGCAGATAGGAAGCGACGGGGGCTTCCTACCGGCCCCCGTCCGGCGCGACCAACTG
CTGCTCGCCCCGGCCGAACGCGCCGACGTCGTCGTCGACTTCTCCTCGACCCCGGTCGGC
TCGGAGCTGTACCTGATCAACGAGGGACCGGACGCCGTGTTCGCGGGGGGCACGGTCGAA
ACCGGCTTCCCCGCTGCGAACCCGAAGACCACCGGGCAGATCATGAAGCTCGTGATCGAA
CCGCTCGTCACGGCGGACACCTCGACGCCTCCCGCACAGCTCACGCTGCCACCCGTCAAG
CCCCTGGGCGCAGCAGACCGCACCCGACAGGTCAGTCTCGGCGAGGTGGCCTCAGCCCGC
GTGCGGGGCGTGAGCCCCCGCGAAGTGCTGCTCGGCGTCATCGGTCCGAACGGCGAACCG
AGGCTGTTGGGATGGGCCGATCCCATCACGGAGAACCCCGCCGTGGGGTCGACGGAGATC
TGGGAGATCCACAACCTGACGAAGGACGCCCACCCCATTCACATCCACGAAGTGCAGTTC
CAGATCGTCGACCGGCAAAAACTTCCGGTCGGCGGCCGTCGACCCCCGGAGGCGTGGGAG
CGCGGTTTCAAGGACACGGTCCTCGCCTACCCGCAGGAGACAACCCGGGTCAAGGTGCGC
TTCAGCCAGACAGGCCGGTACATGTGGCACTGCCACCTCCTGGAACACGAGGACAACGAA
ATGATGCGTCCCCTGCACGTCGGTCCGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006311 Twin-arginine translocation pathway, signal sequence (Conserved_site)
[1-36] PS51318
PS51318   TAT
IPR008972 Cupredoxin (Domain)
[46-270] 6.40000000000004e-57 G3DSA:2.60.40.420 [296-441] 5.30000000000001e-61 G3DSA:2.60.40.420 [498-648] 8.50000000000004e-42 G3DSA:2.60.40.420
G3DSA:2.60.40.420   Cupredoxin
[45-277] 6.69999687625999e-14 SSF49503 [291-435] 6.19998763505952e-26 SSF49503 [496-649] 5.49999338756182e-30 SSF49503
SSF49503   Cupredoxin
IPR011706 Multicopper oxidase, type 2 (Domain)
[557-649] 1.69999999999999e-22 PF07731
PF07731   Cu-oxidase_2
SignalP
[1-29] 0.908 Signal
Eukaryota   
[1-38] 0.906 Signal
Bacteria, Gram-negative   
[1-38] 0.999 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Aran_00020 Aran_00020   Aran_00040 Aran_00040
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