Aran_00060 Aran_00060   Aran_00080 Aran_00080

Aran_00070 : CDS information

close this sectionLocation

Organism
StrainTü303
Entry nameAranciamycin
Contig
Start / Stop / Direction13,039 / 12,254 / - [in whole cluster]
13,039 / 12,254 / - [in contig]
Locationcomplement(12254..13039) [in whole cluster]
complement(12254..13039) [in contig]
TypeCDS
Length786 bp (261 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative polyketide C-9 ketoreductase
Product (GenBank)ketoreductase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • orf7
Reference
ACC
PmId
[17357167] Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. (Chembiochem. , 2007)
comment
aranciamycin生合成gene cluster(ara-cluster)のクローニングとシークエンス報告。

ORF7(261aa): ketoreductase

配列解析のみ。
minimal PKS genesのそばにある。
Related Reference
ACC
Q8VWB5
PmId
[8157619] Nucleotide sequence of the aknA region of the aklavinone biosynthetic gene cluster of Streptomyces galilaeus. (J Bacteriol. , 1994)
comment
Blast 6th, id74%, 1e-105
Streptomyces galilaeus(3AR-33)_aknA
AknA

2-hydroxyaklavinone(polyketide C9の位置にOH基が残っているaklavinone)を産生するS. galilaeus ANR-58では、aknAの配列に変更がある。
ACC
Q56166
NITE
Jado_00110
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 22nd, id70%, 1e-100
Streptomyces venezuelae_ORF5(jadE)
Ketoreductase

--
[PMID: 7881555](1994)
ORF5(=jadEに相当)は配列解析からketoreductaseであると提唱されている。
ORF2-ORF4領域(ORF5も含まれる)を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、
そのplasmid発現株での産物を解析。
JadEはdecaketideだけでなく、octaketideも還元できる。
ACC
Q54812
NITE
Adria_00250
PmId
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 38th, id68%, 1e-93
Streptomyces peucetius_dpsE
Daunorubicin-doxorubicin polyketide synthase
[DoBISCUIT]C-9 ketoreductase

DpsEは、DpsFによる環化に先立って必要なC-9還元を担う、decaketideに特異的なketoreductaseであることが実験で確認されている。

minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
DpsE KRはdecaketidesを還元できるが、octaketidesを還元できない。

close this sectionSequence

selected fasta
>putative polyketide C-9 ketoreductase [ketoreductase]
MVNDDRRVALVTGATSGIGLSVARDLARAGLAVFLCARDTDAVKRTVEELRAVGHEADGT
SCDVRDKTSVRALVDSALGAYGRVDVLVNNAGRNGGGVTADLPDETWYDVIDTNLNSVFL
VTREVLKRSGMRERGWGRVISIASTGGKQGVVLAAPYSASKHGVIGFSKALGKELAPTGV
TVNAVCPGYVETPMAQRVRAGYAAAWETTEEDVLEQFQAKIPLGRYSTPEEVAGLVGYLV
SDTAASITAQALNVCGGLGNY
selected fasta
>putative polyketide C-9 ketoreductase [ketoreductase]
ATGGTGAACGACGATCGGCGGGTGGCCCTCGTCACGGGCGCCACCAGCGGCATCGGGCTG
TCCGTCGCCCGGGACCTGGCCCGCGCGGGCCTGGCGGTGTTCCTCTGCGCCCGGGACACG
GACGCCGTGAAGCGGACGGTGGAGGAACTGCGGGCCGTCGGTCACGAGGCCGACGGCACG
TCGTGCGACGTACGGGACAAGACCTCGGTCCGGGCCCTGGTGGACTCCGCCCTCGGGGCG
TACGGCCGGGTCGACGTCCTGGTGAACAACGCCGGCCGCAACGGCGGCGGGGTCACCGCC
GATCTCCCCGACGAGACCTGGTACGACGTCATCGACACCAACCTCAACAGCGTCTTCCTG
GTCACCCGTGAGGTGCTCAAGCGCTCCGGTATGCGGGAGCGCGGCTGGGGCCGGGTCATC
AGCATCGCCTCCACCGGCGGCAAACAGGGCGTGGTGCTCGCGGCGCCCTACTCGGCGTCC
AAGCACGGGGTGATCGGCTTCTCCAAAGCGCTCGGCAAGGAGCTCGCGCCGACCGGCGTC
ACCGTCAACGCGGTGTGCCCGGGCTACGTCGAGACCCCGATGGCCCAGCGGGTGCGGGCC
GGCTACGCCGCCGCCTGGGAGACCACCGAGGAGGACGTACTGGAGCAGTTCCAGGCCAAG
ATCCCGCTGGGGCGCTACTCCACGCCGGAGGAGGTGGCCGGCCTCGTCGGCTATCTGGTG
AGCGACACGGCGGCGTCCATCACGGCCCAGGCGCTGAACGTCTGCGGCGGCCTGGGCAAC
TACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[1-261] 9.5000102816722e-16 PIRSF000126
PIRSF000126   11-beta-HSD1
[8-175] 7.10000000000008e-35 PF00106
PF00106   adh_short
[82-93] 2.19999900980708e-13 PR00080 [137-145] 2.19999900980708e-13 PR00080 [157-176] 2.19999900980708e-13 PR00080
PR00080   SDRFAMILY
IPR002347 Glucose/ribitol dehydrogenase (Family)
[8-25] 5.50001754266469e-47 PR00081 [82-93] 5.50001754266469e-47 PR00081 [131-147] 5.50001754266469e-47 PR00081 [157-176] 5.50001754266469e-47 PR00081 [178-195] 5.50001754266469e-47 PR00081 [222-242] 5.50001754266469e-47 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[6-258] 6.70000000000005e-82 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[144-172] PS00061
PS00061   ADH_SHORT
SignalP No significant hit
TMHMM No significant hit
Aran_00060 Aran_00060   Aran_00080 Aran_00080
Page top