Aran_00200 Aran_00200   Aran_00220 Aran_00220

Aran_00210 : CDS information

close this sectionLocation

Organism
StrainTü303
Entry nameAranciamycin
Contig
Start / Stop / Direction29,585 / 30,868 / + [in whole cluster]
29,585 / 30,868 / + [in contig]
Location29585..30868 [in whole cluster]
29585..30868 [in contig]
TypeCDS
Length1,284 bp (427 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productglycosyltransferase
Product (GenBank)L-rhamnosyltransferase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • glycosyltransferase; orf21
Reference
ACC
PmId
[17357167] Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. (Chembiochem. , 2007)
[16713002] Cloning, purification and characterization of a functional anthracycline glycosyltransferase. (J Biotechnol. , 2006)
comment
[PMID: 17357167](2007)
aranciamycin生合成gene cluster(ara-cluster)のクローニングとシークエンス報告。

ORF21(427aa): GT(AraGT)

nucleotide-activated deoxysugarsを産生可能なStreptomyces fradiae A0, Streptomyces diastatochromogenes Tu6028でara clusterを発現。

その結果、C-8でmethoxy基を欠いているが、C-7で2-O-methyl-L-rhamnose, L-rhodinose, L-axenose, D-amicetoseをそれぞれ含む新しい化合物aranciamycin A, B, C, Dが検出された。

araGTを不活化したときはglycosylateされたaranciamycin派生体を産生しなかった。

よって、AraGTは上記の糖残基すべての転移を担うことが示され、その基質特異性がゆるいことがわかる。

MaTu and MCF7 cells増殖に対して、L-rhodinose、D-amicetoseを含むaranciamycin B, Dは最高の抑制活性を示した。

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[PMID: 16713002](2006) abstract
上記論文と同じ著者含む。[PMID: 17357167]で引用されている。

AknT and StfGと対照的に、AraGTは活性化のためのpartnerタンパクを必要としない。

close this sectionSequence

selected fasta
>glycosyltransferase [L-rhamnosyltransferase]
MRVLITVPPLDAHFNGSVPLAWALRTAGHEVRVAAQPPLTAGITSAGLTAVPVGDDPGLA
AVMARKGPRIYAHHEGRDLRRAATAPDGPEFLRTSSALLTDAFYSRIGSEETVDDLVAFC
RAWRPDLVVWETFTFAGAIAAHVTGVPHARLLWGPDLFAFMRDALLDHHRRAGVDAGSTP
GADPLRDWITGQLARFGEEFTEAVVRGLWRIDQMPEELRLAPGQHTVPLRYVPYNGPVPA
VVPPWLRHDPDRPRVCLTKGFSVRTIDSPDGRAVTTDDFFDAVADLDAEVVALLDETDRA
ALTTVPANTRVVDFTPLHVLLPTCAAIVHHGGAGTWSTAAVHGVPQLLLASMWDNVFRAV
RTEELGAGLFLPPAELTPAALRGALERLLKEPSFADGADHLRRAMTSQPAPHTVVTELER
LAAQPAA
selected fasta
>glycosyltransferase [L-rhamnosyltransferase]
ATGCGTGTTCTGATCACCGTGCCGCCACTCGATGCCCATTTCAACGGCTCCGTCCCCCTG
GCCTGGGCCCTGCGCACGGCAGGTCACGAGGTTCGCGTGGCCGCACAGCCACCCCTGACC
GCCGGCATCACCTCCGCCGGCCTCACCGCAGTACCGGTCGGCGACGACCCGGGCCTGGCC
GCCGTCATGGCCCGCAAGGGCCCGCGGATCTACGCCCACCACGAGGGCCGGGACCTGCGC
CGGGCCGCCACCGCCCCCGACGGCCCGGAGTTCCTGCGGACCAGCAGCGCCCTGCTCACC
GACGCCTTCTACTCCCGTATCGGCAGCGAGGAGACCGTCGACGACCTGGTCGCCTTCTGC
CGCGCATGGCGGCCCGACCTCGTCGTGTGGGAGACGTTCACCTTCGCCGGAGCGATCGCC
GCACACGTCACCGGCGTCCCGCACGCCCGGCTGCTGTGGGGCCCCGACCTGTTCGCGTTC
ATGCGCGACGCCCTCCTCGACCACCACCGGCGCGCCGGAGTGGACGCGGGCAGCACTCCC
GGCGCCGATCCCCTGCGGGACTGGATCACCGGCCAACTCGCCCGCTTCGGGGAGGAGTTC
ACGGAGGCCGTCGTCCGCGGACTGTGGCGGATCGACCAGATGCCCGAAGAACTGCGCTTG
GCTCCAGGCCAGCACACCGTCCCCCTGCGGTACGTGCCCTACAACGGACCGGTCCCCGCC
GTCGTACCGCCCTGGCTGCGGCACGACCCCGACCGGCCGCGGGTCTGCCTGACCAAGGGG
TTCAGCGTGCGCACCATCGACTCGCCCGACGGACGCGCGGTGACCACGGACGACTTCTTC
GACGCCGTCGCCGATCTCGACGCGGAGGTCGTCGCCCTCCTCGACGAGACCGACCGGGCA
GCGCTGACCACCGTGCCCGCCAACACCCGCGTCGTCGACTTCACCCCGCTGCACGTCCTG
CTGCCCACCTGCGCGGCGATCGTGCACCACGGCGGCGCCGGCACCTGGTCCACCGCGGCC
GTCCACGGAGTGCCCCAGCTGCTCCTGGCCTCCATGTGGGACAACGTCTTCCGGGCCGTA
CGGACGGAGGAACTCGGCGCCGGCCTGTTCCTGCCGCCCGCCGAGCTGACCCCCGCGGCC
CTGCGCGGCGCGCTGGAGCGTCTGCTGAAGGAGCCGTCCTTCGCGGACGGCGCCGACCAC
CTCCGCCGGGCGATGACGTCCCAGCCCGCCCCCCACACGGTCGTCACGGAGCTGGAACGG
CTCGCCGCACAGCCGGCGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
[12-154] 2.1e-12 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[211-310] 1.6e-28 PF06722
PF06722   DUF1205
SignalP
[1-23] 0.245 Signal
Bacteria, Gram-positive   
[1-35] 0.173 Signal
Eukaryota   
TMHMM No significant hit
Aran_00200 Aran_00200   Aran_00220 Aran_00220
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