Fred_00130 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 49344
Entry name	Fredericamycin
Contig	AF525490
Start / Stop / Direction	11,825 / 12,259 / + [in whole cluster] 11,825 / 12,259 / + [in contig]
Location	11825..12259 [in whole cluster] 11825..12259 [in contig]
Type	CDS
Length	435 bp (144 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	putative oxygenase
Gene	fdmK
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	FdmK
Reference	ACC Q2MGC6 PmId [16305230] Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. (J Am Chem Soc. , 2005) comment Fredericamycin(FDM) gene clusterの同定論文。 fdmK: oxygenase prm, grh, rub clusterにhomologueがなく、菌根菌Glomus mosseaeで長鎖分枝鎖脂肪酸のperoxisomal beta-oxidationに関連するFox2のC末領域127aaに有意な配列類似あり。 fdmC-fdmT3のfragmentをS.albusへ導入し、FDM-A産生に十分であることを確認している。 dalta-fdmEFGHIJK mutantでFDM-A産生は完全になくなる。相補で回復。

Sequence

>hypothetical protein [putative oxygenase]
MSVQQEQVTELIDLVQSVPAKEVEGVVATRPGGVDGALTLIFDLLVSEFNPAKAEGEKGV
FQFDIACADGRKHHHVEVVDGTCRTGQGVHDHPDITIGIKFPDMLAMGVGKLPGAKAFLT
GKLKLRGSPLMGTKLGEWFDHPET

>hypothetical protein [putative oxygenase]
ATGAGTGTGCAGCAGGAACAGGTGACCGAACTGATCGACCTGGTCCAGTCGGTGCCGGCC
AAGGAGGTCGAGGGGGTGGTCGCGACCCGCCCCGGTGGTGTCGACGGCGCCCTGACCCTC
ATCTTCGATCTGCTGGTGTCGGAGTTCAACCCGGCCAAGGCCGAGGGCGAGAAGGGGGTG
TTCCAGTTCGACATCGCCTGCGCCGACGGCAGGAAGCACCACCACGTGGAGGTCGTGGAC
GGCACCTGCCGGACCGGTCAGGGAGTGCACGACCACCCCGACATCACCATCGGCATCAAG
TTCCCGGACATGCTGGCGATGGGGGTGGGCAAGCTCCCCGGCGCGAAGGCGTTCCTGACC
GGCAAGCTGAAGCTCCGTGGCAGCCCGCTGATGGGCACGAAGCTGGGTGAGTGGTTCGAC
CACCCCGAGACCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003033 SCP2 sterol-binding domain (Domain) PF02036 SCP2 SSF55718 SCP2
SignalP	Eukaryota
TMHMM	No significant hit

Fred_00120 Fred_00140