Fred_00250 Fred_00250   Fred_00270 Fred_00270

Fred_00260 : CDS information

close this sectionLocation

Organism
StrainATCC 49344
Entry nameFredericamycin
Contig
Start / Stop / Direction24,236 / 22,401 / - [in whole cluster]
24,236 / 22,401 / - [in contig]
Locationcomplement(22401..24236) [in whole cluster]
complement(22401..24236) [in contig]
TypeCDS
Length1,836 bp (611 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Producttranscriptional activator
Product (GenBank)regulatory protein
GenefdmR1
Gene (GenBank)
EC number
Keyword
  • SARP family
Note
Note (GenBank)
  • FdmR1
Reference
ACC
PmId
[16305230] Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. (J Am Chem Soc. , 2005)
[18556785] Identification and utility of FdmR1 as a Streptomyces antibiotic regulatory protein activator for fredericamycin production in Streptomyces griseus ATCC 49344 and heterologous hosts. (J Bacteriol. , 2008)
comment
[PMID: 16305230](2005)
Fredericamycin(FDM) gene clusterの同定論文。

fdmR1: regulator

GrhR2, RubSに似ている。
FdmR1はAsfR-type regulatory proteinであり、たぶんStreptomycesにおいて二次代謝を包括的に調節する

---
[PMID: 18556785](2008)
FdmR1はStreptomyces antibiotic regulatory protein (SARP) familyのメンバーに高い類似性を示す。
fdmR1不活化でFDM生合成不可、相補で回復。

RT-PCRによる転写解析で、fdm gene cluster 28genesのうちfdmRとfdmT2以外の26genesが、FdmR1のpositive control下にあることが判明。
fdmR1過剰発現でFDM力価は5.6倍に改善。

fdm clusterを異種性に発現した時、S. albusではFDM産生可能だが、S. lividansではできない。
しかし、fdmR1を恒常的に発現するとFDM産生をもたらすことができる。そしてputative ketoreductaseをコードするfdmCを、fdmR1と同時に過剰発現することによってさらに拡大される。

close this sectionSequence

selected fasta
>transcriptional activator [regulatory protein]
MLQIGLLGTLQVVHEGVHVTPSAPKVRQVLALLALRANATVPLHQLVEELWEEHPPSSAA
TTLQTYIYQLRKLPGLCDQHRPGPAEDGGAALRTTPGGYRLAVSPDCMDAQRFGELAARG
RVFMEKGSTAEAADLMQRALAVWRGPALSDVAPGPVTGIDALRLEEQRYEVLEERLDADL
LLGRHHRLVSELTGLAADNPTREGLHAKLMLSLYRAGRRPEALEVFQRIRRVLARELGLE
PCAELQRLHQQVLAGDRGLDLPGGRTTVHAPRPGASPMTLPADSPLVGRDADLRHVGRVL
SAEEGAGGPQCVAVAGPPGAGTTAFTVHLAHRLGDSFPDGRLYASFGKGAAPLPVEEVLA
GLLEAVGHQRSELPPHRAGRARMFRNWTEGRRVLLVVDDVATTEQVADLLPAGSGSAALV
AGYRRLYGSQFKARVDLPPLGHERSLELFLDALGRGRGLDDPRGLHTMLDLCGDLPGPTL
EAVGVLALRPHWSVRQLMDWLGRGRLSAVAQQRPHPFHHVVGRCARLEPRLREALAKLAW
EAADDLTVEQAAAVLGKDGDETEEMLEELVELFLLGVGMASDPTTERFFQYRLAPLVRTA
LRAMQPAPSCV
selected fasta
>transcriptional activator [regulatory protein]
GTGCTGCAGATCGGGTTGCTTGGAACCCTGCAAGTCGTCCACGAAGGCGTACACGTCACA
CCGTCCGCACCGAAGGTCCGACAAGTTCTCGCGTTGCTCGCCCTGCGGGCCAACGCCACG
GTCCCGCTGCACCAGCTCGTCGAAGAGCTCTGGGAGGAACATCCGCCGTCCAGCGCCGCG
ACCACGCTCCAGACGTACATCTACCAGTTGCGCAAACTGCCCGGACTCTGCGACCAGCAC
CGTCCCGGGCCGGCCGAGGACGGTGGCGCCGCCCTGCGCACCACCCCCGGCGGCTATCGC
CTCGCCGTCTCCCCCGACTGCATGGACGCACAGCGGTTCGGCGAACTGGCCGCGAGAGGA
AGAGTCTTCATGGAGAAGGGGTCGACGGCGGAGGCCGCGGATCTGATGCAGCGGGCTCTC
GCCGTATGGCGGGGGCCCGCTCTCAGTGATGTGGCCCCCGGCCCCGTCACCGGGATCGAT
GCCCTGCGCCTGGAGGAACAGCGGTACGAGGTCCTGGAGGAGCGGCTCGACGCCGACCTG
CTGCTCGGCCGGCACCACCGCCTGGTCAGTGAATTAACGGGGCTCGCCGCGGACAACCCC
ACCCGAGAGGGGCTGCACGCGAAACTGATGCTCTCCCTCTACCGTGCCGGTCGTCGGCCG
GAGGCCCTGGAGGTCTTCCAGCGCATCCGCCGCGTCCTCGCCCGCGAGCTCGGGCTGGAG
CCGTGCGCGGAACTCCAGCGCCTGCACCAGCAGGTACTCGCCGGCGACCGCGGCCTCGAC
CTCCCGGGCGGCCGGACGACCGTGCACGCACCGCGCCCGGGTGCCTCGCCGATGACGCTG
CCCGCCGACTCGCCCCTGGTGGGCCGGGACGCCGATCTACGGCACGTGGGACGCGTCCTC
TCGGCGGAGGAGGGCGCGGGTGGCCCGCAGTGCGTCGCCGTGGCGGGGCCGCCCGGTGCG
GGCACCACCGCCTTCACCGTCCACCTCGCGCACCGCCTGGGCGACTCCTTCCCCGACGGG
CGCCTCTACGCCTCGTTCGGCAAGGGGGCCGCACCGCTGCCGGTCGAAGAGGTGCTGGCC
GGCCTCCTGGAGGCAGTGGGCCACCAGCGCTCCGAGCTGCCACCGCACCGCGCGGGCCGC
GCCCGGATGTTCCGGAACTGGACGGAGGGCCGCCGCGTGCTCCTGGTGGTGGACGACGTG
GCGACCACCGAGCAGGTCGCCGACCTGCTGCCGGCCGGCTCCGGGTCGGCCGCCCTGGTC
GCCGGGTACCGCAGGCTGTACGGCAGCCAGTTCAAGGCACGGGTGGATCTGCCGCCGCTG
GGCCACGAGAGGTCGCTGGAGCTCTTCCTCGACGCCCTGGGCCGCGGCCGCGGCCTGGAC
GACCCGCGCGGCCTGCACACCATGCTCGACCTCTGCGGCGACCTGCCCGGCCCCACCCTG
GAGGCCGTGGGGGTGCTGGCCCTGCGGCCGCACTGGTCGGTCCGCCAGCTCATGGACTGG
CTCGGCCGCGGCCGGCTCTCCGCCGTGGCCCAGCAGCGGCCGCATCCGTTCCACCACGTG
GTCGGGCGGTGCGCCCGGCTGGAGCCCCGGCTCCGGGAGGCGCTGGCGAAACTGGCCTGG
GAGGCGGCCGACGACCTCACGGTGGAACAGGCCGCCGCCGTCCTGGGGAAGGACGGGGAC
GAGACCGAGGAGATGCTCGAAGAACTGGTCGAGCTCTTCCTCCTCGGCGTCGGCATGGCG
AGCGACCCGACGACGGAACGGTTCTTCCAGTACCGCCTGGCCCCGCTGGTACGGACGGCG
CTGCGCGCCATGCAGCCGGCCCCCAGCTGCGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001867 Signal transduction response regulator, C-terminal (Domain)
[16-101] 1.29999924468179e-09 SM00862
SM00862   Trans_reg_C
[20-73] 8.69999999999999e-13 PF00486
PF00486   Trans_reg_C
IPR005158 Bacterial transcriptional activator domain (Domain)
[108-253] 1.79999754022375e-47 SM01043
SM01043   BTAD
[108-253] 5.10000000000004e-45 PF03704
PF03704   BTAD
IPR011990 Tetratricopeptide-like helical (Domain)
[116-229] 1e-06 G3DSA:1.25.40.10 [525-540] 1e-06 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[24-102] 9.8e-07 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[1-102] 2.59999946718995e-12 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP
[1-39] 0.243 Signal
Bacteria, Gram-negative   
[1-39] 0.395 Signal
Eukaryota   
[1-39] 0.379 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Fred_00250 Fred_00250   Fred_00270 Fred_00270
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