Gilvo_00010 : CDS information

Location

Organism	Streptomyces griseoflavus
Strain	Gö 3592
Entry name	Gilvocarcin
Contig	AY233211
Start / Stop / Direction	4,068 / 799 / - [in whole cluster] 4,068 / 799 / - [in contig]
Location	complement(799..4068) [in whole cluster] complement(799..4068) [in contig]
Type	CDS
Length	3,270 bp (1,089 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative AfsR family transcriptional regulator
Product (GenBank)	putative regulatory protein
Gene
Gene (GenBank)	gilS
EC number
Keyword	SARP family
Note
Note (GenBank)	GilS
Reference	ACC Q7X2F4 PmId [12822997] The complete gene cluster of the antitumor agent gilvocarcin V and its implication for the biosynthesis of the gilvocarcins. (J Am Chem Soc. , 2003) comment gilvocarcin(gil) gene cluster同定のためにクローニングされたcos-G9B3をS.lividans TK24に導入すると、wildレベルのgilvocarcins V and M産生あり。よってgilvocarcin生合成のための完全なclusterが含まれている。 cos-G9B3をサブクローニングしてシークエンス。32.9kbpに26ORFsあり。 supporting info. Table 1 GilS(1089aa): Regulator 配列解析のみ
Related Reference	ACC P25941 PmId [2253887] Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2). (Gene. , 1990) [11952895] afsS is a target of AfsR, a transcriptional factor with ATPase activity that globally controls secondary metabolism in Streptomyces coelicolor A3(2). (Mol Microbiol. , 2002) [17434533] AfsR recruits RNA polymerase to the afsS promoter: a model for transcriptional activation by SARPs. (J Mol Biol. , 2007) comment Blast 6th, id41%, 8e-82 Streptomyces coelicolor_afsR(afsB) Regulatory protein afsR --- [PMID: 2253887](1990) S.lividansのafsBを相補。 pigment生産に関するregulatory proteinとして同定。 2次代謝系のglobal regulatory proteinだとしている。 --- [PMID: 11952895](2002) afsRのターゲットがafsSであることを示し、afsR欠失株ではafsSの転写が起こらないことを示す。 --- [PMID: 17434533](2007) afsRがafsSの上流に結合して、RNA polymeraseのリクルートを介して転写活性化を行うことを示した。

Sequence

>putative AfsR family transcriptional regulator [putative regulatory protein]
MTRRGLRFGLLGTPVLYDGEGPDAPGSVQVTGSPKLRALFVALLLDAGRVVSVEALKDAL
WGGAPPVSALPSLHNHVARLRRLLDDPGRLLTVPTGYLLRVGEGEVDVQVFDAHTAAARA
AHTARDWERVARECAAALALWRGNPLAGMPPEVGGYALVRRLREARLLLLEWRYDAELAL
GGERLADLVPELAALAAEHPLRESYHRQLMLALHRTGRQAEALVVHRDLRARLADELGVE
PGPGVREAHVEVLRGQGERQEHGRCAEAAGGGGVPPRPAQLPPAPAHFTGRDAVRDELIR
VLTRAPRPAAGPPAVVVSGTAGVGKSALAVQVAHTLAERFVDGQLYVNLHGTTPGMTPLT
PAQALSALLRDLGTEPCAVPERPDAAAALLRSLLAPTRTLLVLDDAADAAQVRPLLPGGP
GCAVIVTSRSPLTTLDGAVRVPLGPLSDEDSAALLRAVSGRDGLGAGHPLVALTGRLPLA
LRVVAARLAARRALTPDDLAGQLSATDSRLPHLEYDDLSVRRSLAVAHDALTVSDRETDR
DAALALCRIGALDLPAYGAPLLARLTGTGERRAEAALDRLVDVALVEETAYGRYVPHDLV
RDFARERARAAAGPQTAPREDAALREDAVLREDAVLREDAVLREGAVLREGAVLREGAVL
REGAAPREGAAPREEAVLREDAAPREDAAPREDAGPRTGTREHPAETALRWYAAVAERAL
TAIVEPGPDQDDRRRPTPSQPPGHTAHVASVPPFDDAGAAFAWGDRELENIVTLVGRYAG
EGTGGGGDRGRHDVHIAVLLRLLHPYLLRGGRVPELEVLGRAALRVARRLGDEAAEACAL
GDLAGLHFLTGRTGEALALTDRALEIWRRLGAVSWIRRALNHRGLLLEGLGRYAESERAL
RQSLTHSRQLADPYGEAVTLSHLGNLYEHTDPRAAIDQHRRSLVIGDRIGAVIVRHSAHC
NIGYAHLRLGEPAAALPHFEESLRILAGHGDWHGESQTRLGLVRALRLLGHAGRADAECA
ELLRRADARADRYTGGLARHQYGLLLRERGRVGEAREQWRSALAALDGTDERAVVEELRG
LVAGARPAS

>putative AfsR family transcriptional regulator [putative regulatory protein]
GTGACACGGCGCGGACTGCGGTTCGGACTGCTGGGAACACCGGTGCTGTACGACGGCGAG
GGGCCCGACGCCCCCGGGTCCGTCCAGGTGACCGGAAGTCCCAAACTCCGTGCGCTGTTC
GTCGCGTTGCTGCTCGACGCCGGGCGGGTCGTGTCCGTCGAGGCGCTGAAGGACGCCCTG
TGGGGCGGTGCGCCGCCCGTCTCCGCGCTGCCCTCGCTGCACAATCACGTCGCCCGGTTG
CGGCGGCTGCTCGACGACCCGGGCCGGCTGCTCACCGTGCCCACCGGATATCTGCTGCGG
GTGGGAGAGGGCGAGGTGGACGTCCAGGTCTTCGACGCGCACACCGCAGCCGCCCGCGCC
GCGCACACCGCCCGGGACTGGGAGCGGGTGGCGCGCGAGTGCGCCGCCGCGCTCGCGCTG
TGGCGGGGGAACCCGCTGGCCGGGATGCCCCCGGAAGTGGGCGGCTACGCCCTGGTGCGG
CGGCTGCGCGAGGCGCGGCTGCTGCTCCTGGAGTGGCGGTACGACGCCGAACTGGCCCTG
GGCGGCGAGCGCCTGGCCGATCTCGTGCCCGAACTGGCGGCACTGGCCGCCGAACATCCG
CTGCGGGAGTCGTACCACCGTCAGCTGATGCTCGCGCTGCACCGCACCGGCCGTCAGGCG
GAGGCGCTGGTGGTCCACCGGGATCTGCGGGCCCGCCTGGCGGACGAACTCGGCGTCGAA
CCGGGGCCGGGGGTGCGCGAGGCCCACGTGGAGGTGCTGCGGGGGCAGGGGGAGCGGCAG
GAGCACGGTCGGTGCGCGGAGGCCGCAGGAGGGGGCGGTGTGCCGCCCAGGCCGGCGCAA
CTGCCGCCCGCGCCTGCTCACTTCACCGGGCGGGACGCGGTGCGGGACGAGCTGATCCGT
GTGCTCACCCGTGCCCCCCGGCCCGCCGCCGGGCCCCCTGCCGTCGTCGTCAGCGGTACC
GCTGGTGTGGGGAAGAGCGCGCTCGCCGTCCAGGTCGCACACACACTGGCGGAACGTTTC
GTCGACGGTCAGCTCTACGTCAACCTGCACGGCACGACCCCCGGCATGACCCCCCTCACC
CCCGCCCAGGCCCTCAGCGCTCTCCTGCGCGACCTGGGCACCGAGCCCTGCGCCGTCCCC
GAACGGCCGGACGCGGCCGCCGCCCTGCTGCGCTCGCTGCTCGCGCCCACCCGCACCCTC
CTGGTGCTGGACGACGCCGCGGACGCCGCGCAGGTGCGGCCGCTGCTGCCGGGCGGACCC
GGCTGTGCGGTGATCGTCACCAGCCGTTCGCCGCTCACCACGCTCGACGGTGCCGTCCGT
GTCCCGCTCGGCCCGCTGTCCGACGAGGACAGTGCCGCCCTGCTGCGGGCGGTCTCCGGC
CGGGACGGTCTCGGCGCCGGGCACCCGCTCGTCGCGCTCACCGGCCGGCTTCCGCTGGCC
CTGCGGGTGGTCGCGGCCCGGCTCGCCGCGCGCCGGGCCCTCACGCCCGACGACCTCGCC
GGTCAGCTCTCCGCCACCGACAGCCGGTTGCCGCACCTGGAGTACGACGACCTCAGCGTG
CGCCGCTCGCTGGCCGTCGCGCACGACGCGCTCACCGTCTCCGACCGTGAGACGGACCGC
GACGCCGCCCTCGCCCTGTGCCGCATCGGCGCGCTCGACCTGCCCGCCTACGGCGCGCCG
CTGCTCGCCCGGCTCACCGGCACCGGCGAACGCCGGGCGGAGGCCGCGCTGGACCGGCTC
GTCGACGTGGCCCTCGTGGAGGAGACCGCGTACGGCCGCTACGTCCCCCACGACCTGGTC
CGTGACTTCGCCCGCGAACGAGCCCGAGCGGCGGCGGGTCCGCAGACCGCGCCCCGCGAG
GACGCCGCACTCCGCGAGGACGCCGTACTCCGCGAGGATGCCGTACTCCGCGAGGATGCC
GTACTCCGCGAGGGCGCCGTACTCCGCGAGGGCGCCGTACTCCGCGAGGGCGCCGTACTC
CGCGAGGGCGCCGCACCCCGCGAGGGCGCCGCACCCCGCGAGGAGGCCGTACTCCGCGAG
GATGCCGCACCCCGCGAGGACGCCGCACCCCGCGAGGACGCCGGCCCTCGGACCGGCACG
CGCGAGCACCCCGCCGAGACCGCCCTGCGCTGGTACGCCGCCGTCGCCGAACGCGCCCTC
ACCGCGATCGTCGAACCCGGCCCCGACCAGGACGACCGCCGCCGCCCGACCCCGTCCCAG
CCGCCCGGGCACACCGCTCACGTCGCGTCCGTACCCCCGTTCGACGACGCGGGGGCGGCC
TTCGCCTGGGGCGACCGGGAGCTGGAGAACATCGTCACCCTCGTCGGGCGGTACGCCGGT
GAGGGCACCGGCGGGGGCGGTGACCGGGGCCGTCACGACGTCCACATCGCCGTACTCCTC
CGCCTGCTCCACCCCTACCTCCTGCGCGGCGGCCGGGTCCCCGAGCTGGAGGTGCTCGGG
CGGGCGGCCCTGCGGGTGGCGCGGCGGCTCGGGGACGAGGCCGCCGAGGCGTGCGCGCTG
GGCGATCTGGCGGGGCTGCACTTCCTGACCGGTCGCACCGGCGAGGCCCTCGCCCTCACC
GACCGCGCGCTGGAGATCTGGCGGCGGCTCGGAGCCGTCTCCTGGATCCGGCGCGCCCTC
AACCACCGGGGGCTGCTGCTCGAAGGGCTCGGCCGGTACGCGGAGTCGGAGCGGGCACTG
CGGCAGAGCCTCACCCACTCGCGGCAGTTGGCCGACCCCTACGGCGAGGCCGTCACCCTC
AGCCACCTCGGCAACCTGTACGAGCACACCGATCCGCGCGCGGCGATCGACCAGCACCGG
CGCTCGCTCGTGATCGGGGACCGCATCGGAGCGGTGATCGTGCGGCACTCCGCCCACTGC
AACATCGGCTACGCCCACCTCCGGCTGGGTGAACCGGCCGCCGCCCTGCCGCACTTCGAG
GAGAGCCTGCGCATCCTCGCCGGGCACGGCGACTGGCACGGAGAGTCCCAGACCCGGCTC
GGCCTGGTGCGCGCCCTGCGGCTGCTGGGGCACGCCGGCCGGGCCGACGCCGAGTGCGCC
GAACTGCTGCGCCGCGCGGACGCCCGAGCCGACCGCTACACCGGTGGTCTGGCCCGCCAC
CAGTACGGTCTGCTGCTGCGCGAGCGGGGGCGGGTGGGCGAGGCGCGCGAGCAGTGGCGG
TCGGCGCTCGCCGCGCTGGACGGCACGGACGAGCGGGCCGTGGTGGAGGAGTTGCGCGGA
CTCGTGGCCGGTGCCCGGCCGGCGAGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000767 Disease resistance protein (Family) PR00364 DISEASERSIST IPR001440 Tetratricopeptide TPR-1 (Repeat) PF00515 TPR_1 IPR001867 Signal transduction response regulator, C-terminal (Domain) SM00862 Trans_reg_C PF00486 Trans_reg_C IPR002182 NB-ARC (Domain) PF00931 NB-ARC IPR003593 AAA+ ATPase domain (Domain) SM00382 AAA IPR005158 Bacterial transcriptional activator domain (Domain) PF03704 BTAD SM01043 BTAD IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 TPR-like_helical IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR013105 Tetratricopeptide TPR2 (Repeat) PF07719 TPR_2 IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector IPR019734 Tetratricopeptide repeat (Repeat) SM00028 TPR
SignalP	No significant hit
TMHMM	No significant hit

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