Gilvo_00110 Gilvo_00110   Gilvo_00130 Gilvo_00130

Gilvo_00120 : CDS information

close this sectionLocation

Organism
StrainGö 3592
Entry nameGilvocarcin
Contig
Start / Stop / Direction15,618 / 16,643 / + [in whole cluster]
15,618 / 16,643 / + [in contig]
Location15618..16643 [in whole cluster]
15618..16643 [in contig]
TypeCDS
Length1,026 bp (341 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative DeoR family transcriptional regulator
Product (GenBank)putative repressor
Gene
Gene (GenBank)gilI
EC number
Keyword
Note
Note (GenBank)
  • GilI
Reference
ACC
PmId
[12822997] The complete gene cluster of the antitumor agent gilvocarcin V and its implication for the biosynthesis of the gilvocarcins. (J Am Chem Soc. , 2003)
comment
gilvocarcin(gil) gene cluster同定のためにクローニングされたcos-G9B3をS.lividans TK24に導入すると、wildレベルのgilvocarcins V and M産生あり。よってgilvocarcin生合成のための完全なclusterが含まれている。

cos-G9B3をサブクローニングしてシークエンス。32.9kbpに26ORFsあり。
supporting info. Table 1
GilI (551aa): Repressor

配列解析のみ。詳細記載なし。
Related Reference
ACC
Q54822
NITE
Adria_00110
PmId
[10846224] The dnrO gene encodes a DNA-binding protein that regulates daunorubicin production in Streptomyces peucetius by controlling expression of the dnrN pseudo response regulator gene. (Microbiology. , 2000)
[20122958] The activator/repressor protein DnrO of Streptomyces peucetius binds to DNA without changing its topology. (Int J Biol Macromol. , 2010)
[20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)
comment
Blast 51st, id39%, 1e-44
Streptomyces peucetius_dnrO
Putative repressor
[Adria_00110]transcriptional activator/repressor

---
[PMID: 10846224](2000)
dnrO::aphII mutant strainはDNR生合成不可能。
このmutant strainにdnrN or dnrI を導入すると生合成可能。
dnrI 発現はDnrNが必要なことがわかっているので、dnrN発現にDnrOが必要ということが示される。

DnrOをE.coliで過剰発現し、抽出液でmobility shift DNA-binding assays。
DnrOはoverlapping dnrN/dnrO(p1) promoter regionへ特異的に結合した。

---
その後、別グループでも報告あり。
[PMID: 20122958][PMID: 20529134][PMID: 20158521](2010)
dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。
DnrOはDnrNと競合してfeedback的にDNRの細胞内濃度を調節している。

close this sectionSequence

selected fasta
>putative DeoR family transcriptional regulator [putative repressor]
MIANRTLELLSLLQTQREWTGDGLAERLGVSPRTVRRDINRLRELGYPVTATKGPSGSYR
LSRGARLPPLIVDDEQALAIALSLQTAPASVTGMGDATKRALNSIQELLPPHLAHRLATF
SVEQIENAWELAPPQVDPSLLAQLSSAAQQRDLVRFSYRSIHHDSMQDGEVLAEPHRLVV
WSGRWYLVAYDQQRSSWHAYRVDRIKDLAPTAWRFGEREGPDEDITRFVQNQPDRGDTPD
TWPCWGTVLMECPASLVAKWAPGVASFEAVDDRVTRIQMGAWSWSALIGFLITFSCRFTV
EGPPELVAAARRVMGLIDVGIPSHDPLAEPSSRTPGPSAGR
selected fasta
>putative DeoR family transcriptional regulator [putative repressor]
TTGATCGCCAACCGCACGTTGGAACTCCTCAGCCTGTTACAGACCCAGAGGGAGTGGACC
GGTGACGGGCTGGCCGAGCGGCTGGGTGTGTCCCCGCGGACCGTCCGGCGCGACATCAAC
CGACTCCGCGAGCTGGGCTACCCCGTCACGGCGACGAAAGGCCCCTCCGGCTCCTACCGG
CTGTCCCGCGGAGCGCGTCTTCCTCCCCTGATAGTCGACGACGAGCAGGCCCTCGCCATC
GCCCTGTCCCTGCAGACCGCGCCGGCCTCGGTGACCGGCATGGGAGACGCCACCAAACGT
GCGCTGAACTCCATCCAGGAACTGTTGCCACCTCATCTGGCCCACCGGCTCGCCACCTTC
TCCGTCGAACAGATCGAGAACGCGTGGGAACTCGCTCCGCCGCAGGTCGACCCCTCCCTG
CTCGCACAGCTCAGCAGCGCCGCCCAGCAGCGTGACCTCGTGAGGTTCTCCTACCGCTCC
ATCCACCACGACTCGATGCAGGACGGGGAGGTCCTGGCCGAACCCCACCGGCTCGTCGTC
TGGTCGGGACGCTGGTACCTGGTGGCCTACGACCAGCAGCGGAGCTCATGGCACGCCTAC
CGGGTCGACCGCATCAAGGATCTGGCGCCCACCGCCTGGCGCTTCGGCGAGCGGGAGGGT
CCCGACGAGGACATCACCCGCTTCGTACAGAACCAGCCCGATCGCGGTGACACCCCGGAC
ACCTGGCCCTGCTGGGGCACCGTCCTGATGGAGTGTCCCGCGTCGCTGGTGGCGAAGTGG
GCCCCGGGAGTGGCGAGTTTCGAGGCGGTCGACGACAGGGTCACCCGGATCCAGATGGGC
GCGTGGTCGTGGTCGGCGCTCATCGGCTTCCTGATCACCTTCAGTTGCCGCTTCACCGTC
GAGGGTCCGCCCGAACTCGTCGCCGCGGCGCGGAGGGTGATGGGCCTCATCGACGTCGGG
ATTCCGTCGCACGACCCCCTCGCGGAGCCGTCGAGCCGCACACCCGGCCCCTCGGCCGGC
CGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001034 HTH DeoR N-terminal domain (Domain)
[2-57] PS51000
PS51000   HTH_DEOR_2
[5-58] 8.29999581180008e-06 SM00420
SM00420   HTH_DEOR
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[3-62] 9e-18 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR013196 Helix-turn-helix, type 11 (Domain)
[5-57] 1.4e-15 PF08279
PF08279   HTH_11
IPR018356 Transcription regulator, HTH DeoR-type, conserved site (Conserved_site)
[5-39] PS00894
PS00894   HTH_DEOR_1
SignalP No significant hit
TMHMM No significant hit
Gilvo_00110 Gilvo_00110   Gilvo_00130 Gilvo_00130
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