Gilvo_00140 : CDS information

Location

Organism	Streptomyces griseoflavus
Strain	Gö 3592
Entry name	Gilvocarcin
Contig	AY233211
Start / Stop / Direction	18,760 / 17,693 / - [in whole cluster] 18,760 / 17,693 / - [in contig]
Location	complement(17693..18760) [in whole cluster] complement(17693..18760) [in contig]
Type	CDS
Length	1,068 bp (355 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	putative NDP-glucose synthase
Gene
Gene (GenBank)	gilD
EC number	2.7.7.24
Keyword	D-fucofuranose
Note
Note (GenBank)	GilD
Reference	ACC Q7X2H1 PmId [12822997] The complete gene cluster of the antitumor agent gilvocarcin V and its implication for the biosynthesis of the gilvocarcins. (J Am Chem Soc. , 2003) comment gilvocarcin(gil) gene cluster同定のためにクローニングされたcos-G9B3をS.lividans TK24に導入すると、wildレベルのgilvocarcins V and M産生あり。よってgilvocarcin生合成のための完全なclusterが含まれている。 cos-G9B3をサブクローニングしてシークエンス。32.9kbpに26ORFsあり。 supporting info. Table 1 GilD(355aa): NDP-glucose synthase 配列解析のみ。 D-fucofuranoseの生合成への関与が提唱されている。
Related Reference	ACC Q194R4 NITE Mith_00060 PmId [9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997) comment Blast 13th, id72%, 1e-141 Streptomyces argillaceus_mtmD DTDP-glucose synthase E. coli発現タンパクで以下の2酵素の活性見ている。 mtmD: glucose-1-phosphate:dTTP thymidylyl transferase mtmE: dTDP-D-glucose 4,6-dehydratase mtmD mutant -- accumulates premithramycinone

Sequence

>putative glucose-1-phosphate thymidylyltransferase [putative NDP-glucose synthase]
MKALVLSGGAGTRLRPITHSYAKQLVPVANKPVLFYGLEAIAEAGITEVGIVVGDTAAEI
QQAVGDGSAFGLDVTYIRQAAPLGLAHAVLIAREFLGDDDFVMYLGDNFIVGGIADLVAG
FRESRPDTHILLTKVADPSAFGVVELDDDGKAVALEEKPAAPRSDLALVGVFLFTSVVHE
AVRGIEPSARGELEITDALQWLIDHGYDVRSTTISGYWKDTGNVIDMLEVNRAVLEGAKG
RVDGYVDADSEVIGQVTIEAGAEIRGSRIVGPAVIGANTRITGSYVGPSTSIAADCRIES
SEIEFSIVLAGATVQGVRRVESSLIGRNVEVTPAAGVPAAHRLVLGDHSEVRISS

>putative glucose-1-phosphate thymidylyltransferase [putative NDP-glucose synthase]
ATGAAGGCTCTCGTACTGTCCGGCGGGGCCGGAACCCGCCTGAGGCCCATCACCCATTCC
TACGCCAAGCAGCTCGTCCCCGTAGCCAACAAGCCCGTGCTCTTCTACGGGTTGGAGGCG
ATCGCGGAGGCAGGCATCACCGAGGTCGGCATCGTCGTCGGGGACACGGCCGCCGAGATC
CAGCAGGCGGTCGGCGACGGCTCCGCCTTCGGTCTGGACGTCACCTACATACGGCAGGCG
GCGCCGCTCGGTCTCGCCCATGCCGTCCTGATCGCCCGGGAGTTCCTCGGCGACGACGAC
TTCGTCATGTACCTCGGAGACAACTTCATCGTGGGCGGTATCGCGGATCTCGTGGCCGGG
TTCCGCGAGAGCCGGCCCGACACGCACATCCTCCTGACCAAGGTGGCCGACCCCAGCGCC
TTCGGTGTCGTCGAACTCGACGACGACGGGAAGGCGGTGGCGCTGGAGGAGAAGCCGGCC
GCTCCGCGCAGCGACCTGGCCCTGGTCGGCGTCTTCCTGTTCACCTCCGTGGTGCACGAG
GCCGTCCGGGGGATCGAGCCGTCCGCACGCGGCGAGCTGGAGATCACCGACGCCCTGCAG
TGGCTCATCGACCACGGGTACGACGTCCGCTCCACCACCATTTCCGGTTACTGGAAGGAC
ACCGGGAACGTCATCGACATGCTGGAGGTCAACCGGGCCGTGCTGGAGGGCGCCAAGGGC
CGGGTCGACGGCTATGTCGACGCGGACAGCGAGGTCATCGGCCAGGTCACGATCGAGGCG
GGCGCCGAGATCCGCGGATCGCGGATCGTGGGGCCGGCCGTGATCGGCGCCAACACGCGG
ATCACCGGCTCCTACGTCGGCCCGTCCACGTCGATAGCGGCGGACTGCCGGATCGAGTCC
AGCGAGATCGAGTTCTCCATCGTCCTCGCCGGGGCCACGGTCCAGGGGGTCCGCCGCGTG
GAGAGTTCCCTGATCGGGCGGAACGTCGAGGTGACCCCCGCCGCCGGTGTGCCGGCGGCC
CACCGGCTGGTCCTCGGGGACCACAGCGAGGTGCGTATCTCGTCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family) TIGR01208 RmlA_long
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Gilvo_00130 Gilvo_00150