Bena_00040 : CDS information

Location

Organism	Streptomyces sp.
Strain	A2991200
Entry name	Benastatin
Contig	AM501485
Start / Stop / Direction	4,108 / 2,570 / - [in whole cluster] 4,108 / 2,570 / - [in contig]
Location	complement(2570..4108) [in whole cluster] complement(2570..4108) [in contig]
Type	CDS
Length	1,539 bp (512 aa)

Annotation

Category	4.3 transport
Product	putative drug resistance transporter
Product (GenBank)
Gene	benT
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference	PmId [17439117] Molecular analysis of the benastatin biosynthetic pathway and genetic engineering of altered fatty acid-polyketide hybrids. (J Am Chem Soc. , 2007) comment 該当UniProt entryなし。 --- Benastatin A and B生合成をコードする全遺伝子座のクローニング、シークエンス。 flanking regionsの削除とS. albus, S. lividansでの異種性発現によってclusterが同定されている。 BenT(512aa): transmembrane efflux protein FigureとTableのみ。本文での詳細記載なし。
Related Reference	ACC P42670 PmId [7916693] The pur8 gene from the pur cluster of Streptomyces alboniger encodes a highly hydrophobic polypeptide which confers resistance to puromycin. (Eur J Biochem. , 1993) comment Blast 9th, id54%, 1e-106 Streptomyces alboniger_pur8 Puromycin resistance protein pur8 puromycin合成clusterからpuromycin-resistance gene_pur8を分離し、Streptomyces lividansで発現させ、抗生物質抵抗性を検討。puromycinに高く特異的。 active puromycin effluxに加え、生合成経路の最終中間物であるN-acetylpuromycin分泌への関連があるかもしれない。 TMSs予測は14。上流ORFは逆向きで、抵抗性に関与しない。

Sequence

>putative drug resistance transporter
MTAAPDPRRWWGLAVIGLAQLLVVLDATVMSIALPHAQDSLNMSDGSRQWVITAYTLAFG
GLLLVGGQVADRVGYKRTFVIGVIGFVVASTLGGAAPTAGLLFAARALQGVFAALLAPSA
LALLTTTFHDPGERAKAVGVFGAIAGAGSVVGLIAGGLLTEYLSWRWCLYVNLPPGAVVV
GAALIVLRDRRGNSGARPDLPGVLLACPGLVAVTAGFGAAAARGWGDAMVLGLLAGGAIL
LAVFARWQTITRTPLLPPRIVRSRVRTGAYFTMALVTIGLFALFLSVTYYLQTVLGYSPL
KTGLAFLPAAVGIIVGATGISARLSHRVPPRGLIVPGMLLAAAGMLLLTGLGTEPAYLSH
VLPAEFMVGLGTGTTFMAAIGSASLGVDPRETGIASATLTTAQQIGGSMGVALLNTVASS
AAQRYESARTGAEPEPGAAVERAATVHGFAVASGWVAALLALGAVAVGFVLVTRTQPPES
SIGTGSVERPGDAPPAVPARRQPPANSGGNHP

>putative drug resistance transporter
ATGACTGCCGCACCCGATCCGCGACGCTGGTGGGGACTCGCCGTCATCGGCCTCGCCCAG
CTGTTGGTGGTGCTCGACGCCACGGTCATGAGCATCGCCCTCCCGCACGCCCAGGACAGC
CTCAACATGAGTGACGGCAGCCGGCAGTGGGTCATCACCGCCTACACCCTTGCGTTCGGC
GGACTGTTGCTCGTTGGCGGCCAGGTCGCCGACCGCGTCGGGTACAAGCGCACGTTCGTC
ATCGGTGTCATCGGATTCGTGGTCGCCTCCACGCTGGGCGGCGCGGCACCCACGGCGGGA
CTGCTGTTCGCGGCGCGAGCCCTCCAGGGCGTGTTCGCCGCGCTGCTCGCACCGTCCGCG
CTCGCCCTGCTCACGACGACCTTCCATGACCCCGGCGAACGCGCCAAGGCTGTCGGCGTG
TTCGGCGCGATCGCCGGAGCGGGCTCGGTGGTCGGCCTGATCGCCGGTGGTCTGCTCACC
GAGTACCTGAGCTGGCGGTGGTGTCTGTACGTCAATCTGCCGCCCGGCGCCGTCGTTGTG
GGCGCAGCGCTGATCGTGCTGCGCGATCGGCGGGGGAACTCCGGTGCCCGCCCGGACCTC
CCGGGTGTGCTGCTGGCCTGCCCCGGCCTCGTCGCCGTGACCGCAGGTTTCGGAGCGGCG
GCCGCACGCGGCTGGGGAGATGCCATGGTGCTGGGCCTGCTCGCCGGGGGCGCCATCCTG
CTCGCCGTATTCGCCCGCTGGCAGACCATCACACGGACGCCGCTGTTGCCGCCCCGCATC
GTGCGCAGCCGGGTGCGCACGGGTGCCTACTTCACCATGGCGCTGGTCACCATAGGGCTG
TTCGCCCTGTTCCTGTCCGTCACGTACTACCTGCAGACCGTCCTCGGCTACTCCCCGCTC
AAGACCGGCCTGGCCTTCCTGCCGGCTGCCGTCGGCATCATCGTCGGCGCGACCGGGATC
TCCGCCCGGCTCAGCCACCGGGTGCCACCGCGTGGGCTGATCGTGCCGGGCATGCTGCTG
GCCGCGGCCGGGATGCTGCTGCTCACCGGGCTCGGCACGGAGCCGGCGTATCTCTCGCAC
GTCCTGCCGGCGGAGTTCATGGTGGGGCTGGGCACCGGTACCACCTTCATGGCCGCGATC
GGCAGCGCCTCGCTGGGCGTCGACCCCCGGGAAACAGGCATCGCCTCGGCCACCCTCACC
ACGGCACAGCAGATCGGCGGATCCATGGGCGTCGCGCTGCTGAACACGGTGGCGAGCAGC
GCGGCACAGCGGTACGAGAGCGCGCGCACCGGCGCAGAGCCGGAGCCCGGTGCGGCCGTC
GAACGGGCCGCCACGGTGCACGGGTTCGCGGTGGCATCCGGCTGGGTCGCGGCCCTGCTG
GCCCTGGGAGCGGTCGCCGTGGGGTTCGTGCTCGTCACCCGGACCCAGCCGCCTGAGTCC
TCCATCGGAACCGGATCGGTGGAGCGTCCCGGCGACGCGCCGCCGGCCGTTCCGGCCCGA
CGTCAACCACCCGCGAATTCCGGAGGTAACCACCCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011701 Major facilitator superfamily (Family) PF07690 MFS_1 IPR016196 Major facilitator superfamily domain, general substrate transporter (Domain) SSF103473 MFS general substrate transporter IPR020846 Major facilitator superfamily domain (Domain) PS50850 MFS
SignalP	No significant hit
TMHMM	Transmembrane 13

Bena_00030 Bena_00050