Bena_00170 : CDS information

Location

Organism	Streptomyces sp.
Strain	A2991200
Entry name	Benastatin
Contig	AM501485
Start / Stop / Direction	17,480 / 15,351 / - [in whole cluster] 17,480 / 15,351 / - [in contig]
Location	complement(15351..17480) [in whole cluster] complement(15351..17480) [in contig]
Type	CDS
Length	2,130 bp (709 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein(fragment)
Product (GenBank)
Gene	benX
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference	PmId [17439117] Molecular analysis of the benastatin biosynthetic pathway and genetic engineering of altered fatty acid-polyketide hybrids. (J Am Chem Soc. , 2007) comment 該当UniProt entryなし。 --- Benastatin A and B生合成をコードする全遺伝子座のクローニング、シークエンス。 flanking regionsの削除とS. albus, S. lividansでの異種性発現によってclusterが同定されている。 BenX(709aa): integral membrane export protein FigureとTableのみ。本文での詳細記載なし。
Related Reference	ACC P96687 PmId [15941986] The Bacillus subtilis YdfHI two-component system regulates the transcription of ydfJ, a member of the RND superfamily. (Microbiology. , 2005) comment Blast 76th, id35%, 1e-111 Bacillus subtilis_ydfJ Membrane protein ydfJ RND(resistance-nodulationcell division) superfamilyであるydfJは、YdfHI two-component systemによって調節される。 RND superfamily membersはmultidrug resistance, heavy-metal ion export, transport of oligosaccharides, and solvent tolerance, 幅広い有害化合物への抵抗性などを示すが、ydfJそのものの機能は確かめられていない。 ydfJは12TMSs, MMPL domain2つ、SS(sterol sensing) domainを持つ。

Sequence

>hypothetical protein(fragment)
MASQAAGPAYANVFSVPGTEAEKAQDLVKSAFPDSAGDMDTVVWHVADGTVRDASVRERL
SATLDEIDRLDTVGSVTSPYSQAGTTQISQDGRTAYARITYHKTAEELSVDEAERVVDTA
TAARADGIEVEVSGPVAEKTEEGQAGNIAEFVGLLAAAIVLYLAFGSLLAMLLPLISALF
SVGTGVMTIKLLSHGADVPELAATLSTLIGLGVGIDYALFIITRHRQGLKAGLGPEEAAV
RALNTSGRAVLFAGATVCIALLGLLALRLQFLTGVAIATTTMVVLTMGTSVTFMPALLSL
CGLRVLSRRERRRLAEEGSSQEEPRGLAVRWAAFVQRRPGGLLTAAVVVLAVLTIPFFSL
RLGISDQGNRPASNTTRQAYDMLEDGFGPGFNGPLQLVAELDGQSDKEALRQLADTVRND
ADVASVVALPAQPDATTAVVQVIPKTAPQDEATSELIARLRDTTIPAAERGTTMQVHVGG
QTATFDDFATVMLGKLPWFVLIVVALGFLLLLVAFRSLVIPVTAAVMNLLAAAASFGVLV
ALFQWGWLSGPLGVGGAGPVEAFLPAVMLAVLFGLSMDYQVFLVSRMHEEWVHSHDNPKA
VTVGLTNTSRVINAAAVIMICVFSAFVFRDERIISEFGVGLAAAVALDAFVIRTMLVPAV
MRVLGTANWWLPGWLDKRLPHLAVEPTDEPAPPVSPPPAPAKAPDVRAN

>hypothetical protein(fragment)
GTGGCCAGCCAGGCCGCAGGCCCCGCTTACGCGAACGTCTTCTCCGTCCCCGGCACCGAA
GCGGAGAAGGCACAGGATCTTGTCAAGTCCGCGTTCCCGGACAGCGCGGGCGACATGGAC
ACCGTCGTCTGGCACGTGGCCGACGGGACCGTCCGGGACGCCTCGGTACGCGAACGGCTG
TCCGCGACACTCGATGAGATCGACCGGCTCGACACGGTCGGCTCGGTGACCAGTCCGTAC
TCGCAGGCCGGAACCACTCAGATCAGCCAGGACGGCCGTACCGCCTACGCCAGGATCACG
TACCACAAGACGGCCGAGGAGCTGTCGGTGGACGAGGCCGAGCGCGTCGTCGACACCGCG
ACTGCTGCCCGTGCCGATGGGATCGAGGTCGAGGTCTCGGGGCCCGTAGCGGAGAAGACC
GAGGAGGGGCAGGCCGGCAACATCGCCGAGTTCGTGGGCCTGCTGGCCGCCGCGATCGTG
CTCTATCTCGCCTTCGGGTCGCTGTTGGCGATGCTGTTGCCACTGATCAGCGCGCTGTTC
AGCGTCGGCACCGGGGTAATGACGATCAAGCTTCTGTCGCACGGCGCCGATGTACCGGAA
CTGGCCGCCACCCTGTCGACGCTGATCGGCCTCGGCGTAGGAATCGACTACGCGCTGTTC
ATCATCACGCGCCACCGGCAGGGACTCAAGGCGGGCCTCGGTCCGGAAGAGGCTGCCGTC
CGGGCTCTCAACACGTCGGGCCGCGCCGTTCTCTTCGCCGGTGCCACGGTGTGCATCGCG
CTCCTCGGCCTGCTGGCACTGCGGCTTCAGTTCCTCACGGGCGTCGCCATCGCCACCACC
ACGATGGTGGTACTCACCATGGGCACGTCGGTGACCTTCATGCCGGCGCTGCTCAGCCTG
TGCGGCCTGCGGGTGCTCAGTCGCCGGGAGCGGCGGCGACTGGCCGAAGAGGGCTCGAGC
CAGGAGGAACCGCGCGGACTCGCTGTGCGGTGGGCGGCTTTCGTCCAGCGCCGTCCCGGC
GGGCTCCTGACGGCCGCCGTAGTCGTGCTGGCCGTTCTGACCATCCCGTTCTTCTCGCTC
CGGCTCGGCATCTCCGACCAGGGCAACCGTCCGGCGTCGAACACCACTCGCCAGGCGTAC
GACATGCTGGAGGACGGCTTCGGGCCGGGCTTCAACGGACCGCTGCAACTGGTCGCCGAA
CTCGACGGCCAAAGCGATAAGGAAGCTCTACGGCAACTCGCCGACACCGTACGGAACGAC
GCGGACGTGGCCTCGGTGGTAGCCCTGCCCGCGCAGCCGGACGCCACGACGGCAGTGGTG
CAGGTGATCCCGAAGACCGCCCCCCAGGACGAGGCGACATCGGAACTCATCGCACGATTG
CGCGACACGACCATTCCGGCCGCCGAGCGCGGCACCACGATGCAGGTTCACGTCGGGGGA
CAGACCGCGACGTTCGACGACTTCGCCACCGTCATGCTCGGCAAGCTGCCGTGGTTCGTG
CTGATCGTGGTGGCCCTCGGCTTCCTTCTGCTCCTCGTGGCCTTCCGCAGTCTGGTGATC
CCGGTAACGGCTGCGGTGATGAACCTGCTCGCCGCGGCGGCATCGTTCGGTGTGCTGGTG
GCGCTCTTCCAGTGGGGCTGGCTCTCAGGTCCACTCGGGGTCGGTGGGGCCGGGCCGGTC
GAGGCGTTCCTGCCGGCGGTCATGCTGGCGGTGTTGTTCGGACTGTCGATGGACTACCAG
GTGTTCCTGGTCAGCCGGATGCACGAGGAGTGGGTGCACTCCCACGACAACCCCAAGGCG
GTGACCGTGGGCCTGACGAACACGAGCCGGGTCATCAACGCGGCCGCGGTCATCATGATC
TGCGTGTTCTCGGCGTTCGTGTTCCGCGACGAGCGGATCATCTCCGAGTTCGGCGTGGGC
CTGGCAGCAGCCGTGGCACTGGACGCGTTCGTCATCCGCACCATGCTGGTGCCCGCCGTC
ATGCGGGTGCTGGGCACTGCCAACTGGTGGCTGCCCGGCTGGCTCGACAAGCGGCTCCCC
CACCTGGCGGTCGAACCGACGGATGAACCCGCACCCCCGGTGTCCCCGCCTCCCGCTCCG
GCGAAGGCCCCCGACGTGCGGGCGAACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004869 Membrane transport protein, MMPL type (Domain) PF03176 MMPL
SignalP	No significant hit
TMHMM	Transmembrane 10

Bena_00160 last last