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Chart_00130 : CDS information

close this sectionLocation

Organism
StrainHKI-249
Entry nameChartreusin
Contig
Start / Stop / Direction11,162 / 12,310 / + [in whole cluster]
11,162 / 12,310 / + [in contig]
Location11162..12310 [in whole cluster]
11162..12310 [in contig]
TypeCDS
Length1,149 bp (382 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative glycosyltransferase
Product (GenBank)ChaGT1 protein
Gene
Gene (GenBank)chaGT1
EC number
Keyword
  • D-fucose
Note
Note (GenBank)
Reference
ACC
PmId
[15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005)
comment
chartreusin生合成clusterの解析論文。
異種性にclusterを発現してchartreusin産生を確認。

ChaGT1: Glycosyl transferase

配列解析に基づき、機能予測している。
polyketide aglyconesにdeoxyhexosesを付着させるGlycosyl transferaseに似ていることから、ChaGT1はchartarinへのfucose付着を触媒すると考えられる。
Related Reference
ACC
Q70J67
NITE
Chro_00310
PmId
[16391039] Deoxysugar transfer during chromomycin A3 biosynthesis in Streptomyces griseus subsp. griseus: new derivatives with antitumor activity. (Appl Environ Microbiol. , 2006)
comment
Blast 2nd, id44%, 6e-62
Streptomyces griseus subsp. griseus_cmmGII
Glycosyltransferase

chromomycin gene clusterにある4つのGTs gene (cmmGI, cmmGII, cmmGIII, and cmmGIV)の不活化mutant作製、産物解析。

cmmGII mutantはposition 8でのdisaccharide鎖を欠いた4環化合物産生。
ammGI mutantはposition 8でのdisaccharide鎖の2つめの糖を欠いた3環化合物を産生。

よって、生合成中間体prechromomycin A3の8 positionへCmmGII が1つめ、CmmGI が2つめのD-oliosyl基を取り込むことによりdisaccharideの形成を担う。
ACC
Q9L9F5
PmId
comment
Blast 6th,id38%, 9e-53
Streptomyces sphaeroides_novM
NovM

NovMをサブクローニング、異種性発現、精製し活性測定。
aglycone novobiocic acid + dTDP-L-noviose → desmethyldescarbamoyl novobiocin

close this sectionSequence

selected fasta
>putative glycosyltransferase [ChaGT1 protein]
MRILVTTAPSPGHFFPLVPFAWALRARGHQVLVTMPEQFIAEAGRSGLPAVASVPPIDLR
AAMDSGEGPHPSQREGARDASVEHIGRGFGRLSELTVEGTVAVAERWVPDLVVSEPTEHA
GAVAAAHRGVPLLRHNWGLELPPETEEIAADQMAPTLARLGLPAPAKAGLRVDICPESLQ
RPQARPAQRMRFVPYSGAGVTPPWLLEEPDRPRVCVTLGSVVPMIHGVKVLKELGEELSG
DDMETVIAVPDRFADELGALPPGVRAAAWLPLGLLLTHCTAVVHHGGIGTALTALAFGLP
QVALPHMGDQHATADRLAAAGAAAVVRREDITPAAVAEALRRVRSEPGYAEGARALRAEN
TAAPGLDEVACLAEQLAGAHRK
selected fasta
>putative glycosyltransferase [ChaGT1 protein]
ATGCGCATCCTGGTCACCACCGCCCCGAGCCCGGGGCACTTCTTCCCACTCGTCCCCTTC
GCCTGGGCGCTGCGTGCCCGTGGGCACCAAGTCCTGGTCACCATGCCCGAGCAGTTCATC
GCCGAGGCCGGCCGGTCCGGTCTCCCCGCGGTGGCCTCGGTGCCGCCGATCGACCTGCGC
GCCGCGATGGACTCGGGGGAGGGACCGCACCCGTCGCAGCGCGAGGGGGCCCGGGACGCC
TCCGTGGAGCACATCGGCAGAGGCTTCGGCCGACTGTCGGAGCTGACCGTCGAGGGCACC
GTCGCGGTCGCTGAACGGTGGGTGCCCGACCTCGTCGTGTCGGAGCCGACCGAGCACGCC
GGGGCGGTCGCCGCGGCCCACCGCGGGGTTCCGCTGCTCCGGCACAACTGGGGGCTCGAA
CTGCCCCCGGAGACGGAGGAGATCGCCGCGGACCAGATGGCGCCCACCCTCGCCCGCCTC
GGCCTGCCCGCGCCGGCGAAGGCCGGACTGCGGGTCGACATCTGCCCGGAGAGCCTCCAG
CGGCCGCAGGCACGACCGGCGCAGCGGATGCGGTTCGTGCCCTACAGCGGGGCCGGCGTC
ACACCGCCCTGGTTGCTGGAAGAGCCCGACCGCCCCCGGGTCTGCGTGACGCTCGGCAGC
GTCGTGCCGATGATCCACGGCGTGAAGGTCCTCAAGGAACTGGGGGAGGAACTCTCCGGC
GACGACATGGAGACCGTCATCGCCGTTCCCGACCGCTTCGCCGACGAACTCGGCGCGCTT
CCCCCGGGAGTGCGGGCGGCCGCCTGGCTGCCGCTCGGTCTTCTCCTCACCCACTGCACG
GCGGTCGTGCACCACGGCGGGATCGGCACCGCGCTGACCGCCCTGGCCTTCGGACTGCCC
CAGGTCGCCCTGCCCCACATGGGCGACCAGCACGCCACCGCCGATCGTCTCGCGGCGGCC
GGAGCGGCGGCGGTCGTACGTCGCGAGGACATCACTCCCGCCGCCGTGGCCGAGGCCCTG
CGCCGGGTCCGCAGCGAGCCGGGATACGCGGAGGGCGCCCGCGCGCTGAGGGCGGAGAAC
ACCGCGGCGCCCGGTCTCGACGAAGTCGCGTGCCTCGCCGAGCAGTTGGCTGGCGCCCAC
CGGAAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
[3-136] 1.5e-12 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[172-265] 2.4e-25 PF06722
PF06722   DUF1205
SignalP
[1-23] 0.519 Signal
Bacteria, Gram-positive   
[1-28] 0.85 Signal
Eukaryota   
TMHMM No significant hit
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