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Chart_00230 : CDS information

close this sectionLocation

Organism
StrainHKI-249
Entry nameChartreusin
Contig
Start / Stop / Direction21,638 / 20,898 / - [in whole cluster]
2,058 / 1,318 / - [in contig]
Locationcomplement(20898..21638) [in whole cluster]
complement(1318..2058) [in contig]
TypeCDS
Length741 bp (246 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative C-7 ketoreductase
Product (GenBank)ChaL protein
Gene
Gene (GenBank)chaL
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005)
comment
chartreusin生合成clusterの解析論文。
異種性にclusterを発現してchartreusin産生を確認。

ChaL: C-17 Keto reductase

配列解析に基づき、機能予測している。
AknU, DnrH, and SnoaFに似ている。

--
C-17は環化される前のpolyketide鎖としてのpositionである。
Related Reference
ACC
Q53882
NITE
Dauno_00060
PmId
[8655529] Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5. (J Bacteriol. , 1996)
comment
Blast 2nd, id59%, 5e-69
Streptomyces sp. (strain C5)_dauE
Aklaviketone reductase

recombinant DauE を含んだStreptomyces lividans TK24 の抽出液は、
aklaviketone → aklavinone
maggiemycin → epsilon-rhodomycinone(RHO)
7-oxodaunomycinone → daunomycinone
へのNADPH-specificな変換を触媒した。
これらの変換はすべてposition 7でのketo基の還元。

close this sectionSequence

selected fasta
>putative C-7 ketoreductase [ChaL protein]
MRGIVITGGGTGIGRAAAHAFADAGDAVLIVGRSADTLAETAKGRDGIHTLPLDLTEPDA
AAVVVETARRQLGRLDVLVNNAGIASFAGLGSLEPASVEAQIRTNLIAPILLTQQALDSL
EETRGLVVNISSAGSIGLRTIPDNSVYCATKAGLDVLTRTWSVELAPRGIRVIGVAPGVT
DTGVALRAGVSPAQYEAFMERMRAEIPLGRVGAPEDIAWWIVTLASADGGYLNGAVIPVD
GALSVT
selected fasta
>putative C-7 ketoreductase [ChaL protein]
ATGCGAGGAATCGTCATCACCGGCGGCGGCACGGGCATCGGCCGTGCCGCCGCGCACGCT
TTCGCCGACGCCGGCGACGCGGTCCTGATCGTCGGGCGGTCGGCGGACACGCTCGCCGAG
ACCGCGAAGGGCCGGGACGGCATCCACACCCTCCCGCTCGACCTGACCGAGCCGGACGCG
GCGGCCGTCGTCGTCGAAACCGCTCGACGTCAGCTCGGGCGGCTCGACGTGCTCGTCAAC
AACGCCGGGATCGCGAGCTTCGCCGGCCTCGGCTCGCTGGAACCGGCCTCGGTCGAGGCG
CAGATCAGGACCAATCTGATCGCGCCGATCCTGCTCACCCAACAGGCCCTGGACTCGCTG
GAGGAGACCCGCGGTCTCGTCGTCAACATCAGTTCGGCCGGGTCGATCGGGCTGCGCACC
ATCCCGGACAACTCGGTGTACTGCGCCACGAAGGCGGGCCTGGACGTGCTCACCCGCACC
TGGTCCGTCGAGCTGGCCCCGCGCGGCATCCGGGTCATCGGCGTCGCGCCGGGTGTCACC
GACACCGGTGTGGCGCTGCGGGCCGGTGTGTCCCCGGCCCAGTACGAGGCGTTCATGGAG
CGGATGCGGGCGGAGATCCCGCTCGGCCGGGTCGGTGCTCCGGAGGACATCGCCTGGTGG
ATCGTCACGCTGGCCTCGGCGGACGGGGGGTACCTCAACGGCGCGGTGATACCGGTGGAC
GGCGCGCTGTCGGTCACGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[73-84] 1.09999909120787e-11 PR00080 [125-133] 1.09999909120787e-11 PR00080 [147-166] 1.09999909120787e-11 PR00080
PR00080   SDRFAMILY
[4-165] 3.8e-28 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[3-20] 7.40000780398342e-37 PR00081 [73-84] 7.40000780398342e-37 PR00081 [119-135] 7.40000780398342e-37 PR00081 [147-166] 7.40000780398342e-37 PR00081 [168-185] 7.40000780398342e-37 PR00081 [207-227] 7.40000780398342e-37 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[4-245] 1.9e-70 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-20] 0.805 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
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