Chart_00245 : CDS information

Location

Organism	Streptomyces chartreusis
Strain	HKI-249
Entry name	Chartreusin
Contig	AJ786383
Start / Stop / Direction	22,642 / 23,829 / + [in whole cluster] 3,062 / 4,249 / + [in contig]
Location	22642..23829 [in whole cluster] 3062..4249 [in contig]
Type	CDS
Length	1,188 bp (395 aa)

Annotation

Category	3.3 modification reduction
Product	putative oxidoreductase
Product (GenBank)
Gene	chaZ
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference	PmId [15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005) comment UniProtにentryの登録なし。 ChaZとして登録されているentry(Q4R0K8)は、実際にはChaEに相当。 --- [PMID: 15911378](2005) chartreusin生合成clusterの解析論文。異種性にclusterを発現してchartreusin産生を確認。 ChaZ: FAD-dependent monooxygenase chaZを不活化した株ではanthracyclic polyketideであるresomycin Cを蓄積。 chaABC null mutantを使ったresomycin Cとの生体内変換実験は成功しておらず、resomycin Cがshunt産物である可能性あり。
Family/Domain	FD IPR002938, IPR003042 comment [IPR002938] Monooxygenase, FAD-binding (Domain) [IPR003042] Aromatic-ring hydroxylase-like (Domain)

Sequence

>putative oxidoreductase
MANVAVIGAGTGGLCLAQALHAAGIEVTVHERHRTPTGALHGYRVRINPTGARALRRCLP
AQVWETFLSRAGRIGQEFGFLTERLRELAVLDGGPDAEPHYSAERGMLREVLLTGLEDVV
RFGACFERYERTQDGRFECHFADGTSTTADLVVGADGANSRVRAQYLPHARRVDIGMVSI
VGRLALPVAEQLLPERLSSRPNSVVAPGGTGMFVARHEPGTDEGYLMWAYGAGAHRYPAD
VETLPGDQLAELVDGLTGSWHPALRAMVRETDPGTVNAVPIRTSVPPDPWPATRVTLLGD
AIHSMTPLRGVGANMALNDAALLARELTGSADTRLAVSRYEDAMRRDGFRAVSESLRAAR
MFVSESTTQRLLFKTVLRTASAVPPLRKALFAKLA

>putative oxidoreductase
ATGGCGAACGTCGCGGTGATCGGCGCGGGGACGGGCGGACTCTGCCTGGCCCAGGCACTC
CACGCGGCCGGCATCGAGGTGACCGTCCATGAACGGCACCGCACCCCGACCGGCGCGCTG
CACGGCTACCGGGTCCGCATCAATCCGACCGGAGCCCGTGCCCTGCGTCGTTGCCTGCCC
GCCCAGGTGTGGGAGACGTTCCTGTCCCGGGCCGGGCGGATCGGGCAGGAATTCGGATTC
CTCACCGAACGACTGAGGGAACTCGCCGTCCTCGACGGCGGACCGGACGCCGAACCGCAC
TACTCCGCCGAGCGCGGCATGCTGCGGGAGGTCCTGCTGACGGGGCTGGAGGACGTGGTC
CGGTTCGGCGCCTGCTTCGAGAGGTACGAGCGTACCCAGGACGGCCGGTTCGAGTGCCAC
TTCGCCGATGGCACCAGCACCACGGCCGACCTCGTCGTCGGCGCGGACGGCGCCAACTCC
CGTGTCCGCGCCCAGTATCTGCCGCACGCGCGCCGCGTCGACATCGGGATGGTCTCGATC
GTCGGCCGCCTCGCCCTGCCCGTCGCCGAACAACTGCTACCCGAGCGTCTGAGCTCCCGC
CCCAACTCGGTCGTCGCGCCCGGAGGCACGGGGATGTTCGTCGCACGGCACGAGCCCGGC
ACGGACGAGGGGTACCTCATGTGGGCTTACGGCGCCGGAGCACACCGCTATCCCGCGGAC
GTCGAGACCCTGCCCGGCGATCAACTGGCCGAACTGGTCGACGGGTTGACCGGATCCTGG
CACCCCGCACTGCGCGCGATGGTCCGCGAGACCGACCCGGGCACGGTCAACGCGGTGCCC
ATCCGCACCTCGGTTCCCCCGGACCCCTGGCCGGCGACCCGGGTGACCCTCCTCGGCGAC
GCCATCCACAGCATGACGCCACTGCGCGGCGTGGGCGCGAACATGGCACTCAACGACGCC
GCGCTCCTCGCCCGCGAACTCACCGGCAGCGCGGACACGCGCCTGGCCGTGAGCCGGTAC
GAGGACGCGATGCGGCGGGACGGCTTCCGCGCGGTCTCCGAGTCGCTGCGCGCCGCCCGG
ATGTTCGTGTCCGAGAGCACGACACAGCGGCTGCTGTTCAAGACGGTGCTCCGGACCGCA
TCCGCCGTACCCCCGCTCCGCAAGGCATTGTTCGCCAAGCTCGCTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002938 Monooxygenase, FAD-binding (Domain) PF01494 FAD_binding_3 IPR003042 Aromatic-ring hydroxylase-like (Domain) PR00420 RNGMNOXGNASE
SignalP	Eukaryota Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	No significant hit

Chart_00240 Chart_00250