Chart_00300 : CDS information

Location

Organism	Streptomyces chartreusis
Strain	HKI-249
Entry name	Chartreusin
Contig	AJ786383
Start / Stop / Direction	29,863 / 30,789 / + [in whole cluster] 10,283 / 11,209 / + [in contig]
Location	29863..30789 [in whole cluster] 10283..11209 [in contig]
Type	CDS
Length	927 bp (308 aa)

Annotation

Category	4.3 transport
Product	putative ABC transporter metal-binding protein
Product (GenBank)	ChaT2 protein
Gene
Gene (GenBank)	chaT2
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q4R0K2 PmId [15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005) comment chartreusin生合成clusterの解析論文。異種性にclusterを発現してchartreusin産生を確認。 ChaT2: ABC-type metal ion transport system 配列解析に基づき、機能予測している。 ChaT2-T5はKineococcus radiotolerans由来ABC ion transporters componentsに似ている。 ChaT2は表面付着でたぶん機能する。
Family/Domain	FD IPR006127 comment [IPR006127] ABC transporter metal-binding lipoprotein (Family) function; metal ion binding

Sequence

>putative ABC transporter metal-binding protein [ChaT2 protein]
MPLSSPRVHAALLTGTCLTLLVGCAGSSDTAGVHGPAAASVIPVVASTNVYGDLARRIGG
AKVQVTSIISDPDQDPHSYEASTRNQLALSKAEIVIENGGGYDDFVDRMLKSAGSSPGVI
NAVEVSGKTAPKGGELNEHVWYDFPTVAELADRIAIALGKADPADAATFTENAKDFVSLL
QPLERKEARIKAEHGGEAVAITEPVPLYMIEASGLRNATPEEFSEAIEEGDDVSPRTLQS
TLALFTGKKVEALVYNEQTSGPQTEKAERVAEAAGIPVVPVTETLPKGKDYLAWMTANVD
ALASALAK

>putative ABC transporter metal-binding protein [ChaT2 protein]
ATGCCCCTGTCCTCACCCCGCGTGCACGCGGCCCTGCTGACCGGCACCTGCCTGACTCTC
CTCGTCGGCTGTGCCGGCTCCTCGGACACCGCCGGGGTGCACGGTCCCGCCGCGGCCTCC
GTCATCCCCGTCGTGGCCTCGACGAACGTCTACGGGGACCTGGCCAGGCGAATAGGCGGC
GCCAAGGTCCAGGTCACCTCGATCATCAGCGACCCCGACCAGGACCCGCACTCCTACGAG
GCGAGCACCCGCAACCAACTGGCGCTGTCCAAGGCCGAGATCGTCATCGAGAACGGCGGC
GGCTACGACGACTTCGTCGACCGCATGCTGAAGAGCGCGGGCTCGTCCCCCGGCGTGATC
AACGCCGTCGAGGTGTCAGGGAAGACCGCCCCGAAGGGCGGCGAGCTCAACGAGCATGTC
TGGTACGACTTCCCGACGGTCGCCGAACTCGCCGACCGCATCGCCATCGCACTCGGCAAG
GCCGACCCGGCCGACGCCGCGACCTTCACCGAGAACGCGAAGGACTTCGTAAGCCTCCTT
CAGCCGCTGGAGCGCAAAGAGGCCCGGATCAAGGCCGAGCACGGCGGCGAGGCGGTGGCC
ATCACCGAGCCCGTCCCGCTGTACATGATCGAGGCCAGTGGGCTGAGGAACGCCACACCC
GAGGAGTTCAGCGAGGCGATCGAGGAAGGCGACGATGTCTCCCCGAGGACCCTCCAGAGC
ACCCTGGCGCTGTTCACCGGCAAGAAGGTCGAGGCGCTGGTCTACAACGAGCAGACCTCC
GGCCCGCAGACCGAGAAGGCGGAGCGCGTGGCCGAGGCCGCCGGCATCCCGGTCGTGCCC
GTCACCGAGACCCTCCCCAAGGGCAAGGACTACCTCGCCTGGATGACCGCCAACGTGGAC
GCGCTCGCGAGCGCGCTGGCCAAGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006127 ABC transporter, metal-binding lipoprotein (Family) PF01297 SBP_bac_9
SignalP	Eukaryota Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Chart_00290 Chart_00310