Ello_00110 Ello_00110   Ello_00130 Ello_00130

Ello_00120 : CDS information

close this sectionLocation

Organism
StrainTü2353
Entry nameElloramycin
Contig
Start / Stop / Direction10,167 / 11,402 / + [in whole cluster]
10,167 / 11,402 / + [in contig]
Location10167..11402 [in whole cluster]
10167..11402 [in contig]
TypeCDS
Length1,236 bp (411 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase chain length factor subunit
Product (GenBank)ketoacyl synthase beta
Gene
Gene (GenBank)elmL
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[18310024] Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster. (Microbiology. , 2008)
comment
elloramycin clusterの同定論文。
elmLについては配列解析のみ。

elmL: Ketoacyl synthase beta

elmK, elmL, elmMがコードするタンパクはtetracenomycin C minimal PKSに似ており、decaketide形成を担うだろうと述べている。
Related Reference
ACC
Q54174
NITE
Urd_00130
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 5th, id62%, 1e-127
Streptomyces fradiae_urdB
Putative chain length determinant

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdBは芳香族PKS gene clustersのCLFに似ている。
UrdAとUrdBはid36%で似ているが、UrdBはactive site cysteineを持っていない。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。
ACC
Q56164
NITE
Jado_00090
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 14th, id62%, 1e-123
Streptomyces venezuelae_jadB
Chain length determinant

---
[PMID: 7881555](1994)
ORF2(=JadBに相当)は配列解析からtype II PKSのCLFであると提唱されている。
ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
jadBもKS-betaとして使用されている。
ACC
Q54496
NITE
Nogl_00380
PmId
[8668120] A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway. (Mol Gen Genet. , 1996)
comment
Blast 15th, id62%, 1e-122
Streptomyces nogalater_snoa2
Snoa2
[DoBISCUIT]polyketide synthase chain length factor subunit

S. nogalaterのanthracycline生合成gene clusterを解析。
11kb領域に11ORFs(snoEDCBA123XYZ)が同定されている。

sno2: CLF

aclacinomycin生合成がブロックされたS.galilaeus mutantsの相補によりminimal PKSを確認している。

close this sectionPKS/NRPS Module

CLF13..369

close this sectionSequence

selected fasta
>polyketide synthase chain length factor subunit [ketoacyl synthase beta]
MTGSDTEDGSTGWITGLGVVAPNGIGAEEYWKATLEGRSGLRTITGFDAGQYPVRVAGEV
GTFADEPSLSGRILPQTDRMTRYALVASDWALADSGVRTDEHDGFSTGVITASAAGGFEF
GQRELQKLWGSGPGEVSAYQSFAWFYAVNTGQISIRHGLRGHSSVAVAEQAGGLDAVAQA
TRLVDHGTLRVAVAGGFEAPVSPWGLVAQIPSGLLSTVADPARAYLPFDPDASGWVPGEG
GAALVVERAADARARGADHVYGRIAGHASTFDPRPGLGRPPALARAVRTALDRARTHPGD
IDVVFADAAGLPEQDAAEVAALTEVFGSRKVPVTAPKTMTGRLYSGGGALDTATALLALR
DGVVPPTVGTRTAPPELDLVLGAPRDLPLRNALVLARGTGGFNSAVVVARV
selected fasta
>polyketide synthase chain length factor subunit [ketoacyl synthase beta]
GTGACCGGCTCCGACACCGAGGACGGCTCCACCGGCTGGATCACCGGCCTGGGGGTCGTG
GCGCCCAACGGCATCGGCGCGGAGGAGTACTGGAAGGCCACGCTGGAAGGCCGCAGCGGC
CTTCGCACGATCACCGGATTCGACGCCGGGCAGTACCCCGTACGGGTCGCCGGAGAGGTC
GGGACCTTCGCCGACGAACCGAGCCTGTCCGGGCGCATCCTCCCGCAGACCGACCGGATG
ACCCGGTACGCACTGGTCGCCAGCGACTGGGCCCTGGCGGACTCCGGGGTCCGCACCGAC
GAGCACGACGGGTTCTCCACCGGAGTGATCACGGCAAGTGCCGCCGGCGGCTTCGAGTTC
GGCCAACGGGAACTGCAGAAGCTGTGGGGGAGCGGGCCCGGAGAGGTGAGCGCCTACCAG
TCGTTCGCCTGGTTCTACGCCGTGAACACCGGCCAGATCTCCATCCGGCACGGCCTGCGC
GGCCACAGCTCGGTGGCGGTCGCCGAGCAGGCCGGCGGCCTCGACGCCGTGGCGCAGGCC
ACCCGGCTCGTCGACCACGGCACCCTGCGCGTCGCCGTCGCCGGCGGGTTCGAGGCGCCG
GTGTCCCCCTGGGGCCTCGTCGCCCAGATCCCCAGCGGCCTGCTGAGCACCGTGGCCGAC
CCCGCCCGCGCCTATCTGCCCTTCGACCCGGACGCCTCCGGGTGGGTGCCCGGCGAGGGC
GGCGCCGCCCTGGTCGTCGAACGGGCGGCGGACGCCCGCGCCCGCGGAGCGGACCACGTC
TACGGCCGGATCGCCGGACACGCCTCGACCTTCGACCCGCGCCCCGGCCTGGGACGCCCG
CCCGCCCTCGCCCGGGCCGTCAGGACCGCCCTCGACCGTGCCCGTACACATCCCGGCGAC
ATCGATGTCGTCTTCGCGGACGCCGCCGGGCTCCCGGAGCAGGACGCCGCCGAAGTGGCG
GCCCTCACCGAGGTGTTCGGCTCCCGGAAGGTGCCCGTCACCGCCCCGAAGACCATGACC
GGACGGCTCTACTCCGGCGGTGGCGCCCTCGACACCGCCACGGCCCTGCTCGCCCTGCGC
GACGGCGTCGTCCCGCCCACCGTGGGCACGCGCACCGCCCCGCCCGAGCTCGACCTCGTG
CTCGGCGCTCCCCGCGATCTCCCCCTGAGGAACGCCCTCGTCCTCGCCCGCGGGACCGGC
GGCTTCAACTCCGCCGTCGTCGTCGCCCGCGTCTGA
CLF13..369
CLF37..1107

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[13-252] 1.99999999999999e-51 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[261-369] 1.8e-26 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[14-266] 5.30000000000001e-51 G3DSA:3.40.47.10 [279-410] 3.60000000000002e-37 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[9-257] 3.1999989904635e-57 SSF53901 [220-411] 1.49999648196323e-48 SSF53901
SSF53901   Thiolase-like
SignalP
[1-27] 0.234 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Ello_00110 Ello_00110   Ello_00130 Ello_00130
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