Grana_00010 : CDS information

Location

Organism	Streptomyces violaceoruber
Strain	Tü22
Entry name	Granaticin
Contig	AJ011500
Start / Stop / Direction	2,400 / 1,099 / - [in whole cluster] 2,400 / 1,099 / - [in contig]
Location	complement(1099..2400) [in whole cluster] complement(1099..2400) [in contig]
Type	CDS
Length	1,302 bp (433 aa)

Annotation

Category	5.1 general function
Product	putative serine/threonine protein kinase
Product (GenBank)	putative serine-threonine protein kinase
Gene
Gene (GenBank)	gra-orf7
EC number
Keyword
Note	It is supposed that this ORF is not involved in granaticin biosynthesis.
Note (GenBank)
Reference	ACC Q9ZA50 PmId [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) comment entire granaticin gene cluster(gra cluster)の報告。 gra-orf7の産物は、gra-clusterの外側に当たるだろうとの記述あり。
Related Reference	ACC P54739 PmId [7883195] Cloning, sequencing and expression of serine/threonine kinase-encoding genes from Streptomyces coelicolor A3(2). (Gene. , 1995) comment Blast 35th, id37%, 7e-34 Streptomyces coelicolor_pkaA Serine/threonine-protein kinase pkaA Eukaryote Ser/Thr kinaseの配列を元にPCRでSCOからSer/Thr kinaseをクローニング。 PknAとPknBはよくにており、さらに隣り合って存在している。 E.coliで過剰発現させて、In vitroで活性確認。 PknAの少なくともC末100aaはprotein kinase活性に必須でない。

Sequence

>putative serine/threonine protein kinase [putative serine-threonine protein kinase]
MGEVFAGRYELIDPIGRGGVGAVWRAWDHRRRRYVAAKVLQQSDAHTLLRFVREQALRID
HPHVLAPASWAADDDKVLFTMDLVAGGSLAHVIGDYGPLPPRFVCTLLDQLLSGLAAVHA
EGVVHRDIKPANILLDVTGTGRPHLRLSDFGISMRKGEPRLTETNYVVGTPGYFAPEQMM
GAEPDFPADLFAVGLVALYLLQGRKPDSRALIEYFLAHGTPGAPQGIPEPLWQVLAGLLQ
PDPQARFKTATGVRKALTAAVEMLPEAGPGEEPVEVFDQIGPCPPASARTARAPSPAAPA
PVRVQRHPEEPQQPQQPLPTLSETGSFHLPPPPRQQPAPPPQPVAAPAPHPAAVTPLAAH
TDPALAYVPTAAVPYEQASTRSYTAHAPRIPAQASPAAQSPAQRPGPPPKVAIPVLFVAL
ICFAVGIWALTQT

>putative serine/threonine protein kinase [putative serine-threonine protein kinase]
ATGGGTGAGGTCTTCGCGGGTCGGTACGAACTGATCGACCCGATCGGACGCGGTGGCGTA
GGAGCGGTCTGGCGCGCGTGGGACCACCGGCGCCGCCGCTATGTGGCGGCCAAGGTGCTC
CAGCAGAGCGATGCCCACACACTGCTCCGCTTCGTCCGGGAGCAGGCGCTCCGGATCGAC
CACCCCCACGTTCTCGCCCCGGCCAGCTGGGCCGCGGACGACGACAAGGTGCTGTTCACC
ATGGATCTGGTGGCCGGCGGCTCCCTGGCGCACGTCATCGGCGACTACGGCCCGCTGCCG
CCGCGCTTCGTCTGCACGCTGCTGGATCAGTTGCTCTCCGGCTTGGCCGCGGTGCACGCG
GAGGGGGTCGTGCACCGCGACATCAAGCCGGCGAACATCCTGCTCGACGTCACCGGGACC
GGGCGGCCGCATCTGCGGCTGTCCGATTTCGGCATCTCGATGCGCAAGGGCGAACCCCGC
CTGACCGAGACCAACTACGTGGTCGGGACGCCCGGTTATTTCGCGCCCGAGCAGATGATG
GGGGCGGAACCCGACTTCCCGGCCGACCTCTTCGCGGTCGGTCTCGTCGCGCTCTATCTG
CTCCAGGGCCGGAAGCCCGACTCCCGCGCCCTCATCGAGTACTTCCTGGCGCACGGGACG
CCGGGTGCCCCCCAGGGCATTCCGGAACCGCTGTGGCAGGTCCTCGCCGGTCTGCTCCAG
CCCGACCCGCAGGCCCGTTTCAAGACGGCCACGGGCGTGCGCAAGGCCCTGACCGCGGCG
GTCGAGATGCTGCCCGAGGCCGGCCCGGGCGAGGAGCCGGTCGAGGTCTTCGACCAGATC
GGCCCGTGCCCCCCGGCTTCGGCCCGAACGGCCCGAGCCCCTTCGCCAGCGGCCCCCGCC
CCCGTACGCGTCCAGCGGCACCCCGAGGAGCCGCAGCAGCCCCAGCAGCCGCTGCCGACC
CTTTCCGAGACCGGCAGCTTCCACCTGCCCCCGCCCCCGCGGCAGCAGCCCGCACCGCCC
CCGCAGCCGGTCGCCGCCCCCGCGCCGCACCCGGCCGCCGTCACGCCCCTCGCCGCGCAT
ACCGACCCCGCCCTCGCCTACGTCCCCACGGCCGCGGTGCCGTACGAACAAGCCTCCACC
CGTTCGTACACCGCTCACGCCCCGCGGATTCCCGCCCAGGCCTCACCGGCGGCGCAGAGC
CCGGCACAGCGGCCGGGACCGCCCCCGAAGGTGGCGATCCCGGTCCTGTTCGTCGCGCTG
ATCTGTTTCGCGGTCGGCATCTGGGCCCTCACCCAGACCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000719 Protein kinase, catalytic domain (Domain) PF00069 Pkinase PS50011 PROTEIN_KINASE_DOM IPR008271 Serine/threonine-protein kinase, active site (Active_site) PS00108 PROTEIN_KINASE_ST IPR011009 Protein kinase-like domain (Domain) SSF56112 Kinase_like
SignalP	No significant hit
TMHMM	Transmembrane 1

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