Grana_00130 Grana_00130   Grana_00150 Grana_00150

Grana_00140 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction16,510 / 17,100 / + [in whole cluster]
16,510 / 17,100 / + [in contig]
Location16510..17100 [in whole cluster]
16510..17100 [in contig]
TypeCDS
Length591 bp (196 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative transcriptional regulator
Product (GenBank)putative transcriptional activator
Gene
Gene (GenBank)gra-orf20
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
entire granaticin gene cluster(gra cluster)の報告。

ORF20: Transcriptional activator in redox control (Regulation)

配列解析のみ。E.coli_SoxRに似ている。
SoxRは分かれて転写されるSoxSと共に働くが、ORF20にSoxS homologは隣接していない。
ORF19産物はおそらくORF20産物と一緒に、細胞の酸化還元電位の変化に応答してgranaticinへの抵抗性を調節するかもしれない。
Related Reference
ACC
E3T498
PmId
[21604555] [Function of the granaticin biosynthetic gene orf20 from Streptomyces vietnamensis]. (Wei Sheng Wu Xue Bao. , 2011)
comment
granaticin産生できるStreptomyces vietnamensisのgra-orf20での実験あり(abstract)。
(E3T498)に相当するが、UniProt ver.26.0には含まれていない。

Blast id 83.16%, 3e-87
Streptomyces vietnamensis_ORF Names: gra-orf20
Putative transcriptional activator

orf20はE.coliでのsoxR geneを補完できる。しかし、S.vietnamensisにおける抗酸化ストレス応答調節に関連しない。その代り、granaticin産生でのnegativeな効果を課す(orf20削除でgranaticin産生増加)。

close this sectionSequence

selected fasta
>putative transcriptional regulator [putative transcriptional activator]
MTVARPAHHVTELTVGQLSARSGVAVTALHFYESKGLIKSRRTPGNQRRYTRDTLRRISV
IRVAQRMGIPLSTVAEALAELPEERTPTREDWARLSRRWRADLNERIEQLLALRDGLEDC
IGCGCLSVRDCPLTNPYDELGEEGPGPRRLLREPTGAAARGGARGRARTARTAERTAPVA
DPCGGVSPSCQGGATD
selected fasta
>putative transcriptional regulator [putative transcriptional activator]
ATGACCGTCGCCCGGCCCGCGCACCACGTCACCGAACTCACCGTCGGACAGCTGTCGGCG
CGCAGCGGCGTCGCGGTCACGGCCCTGCACTTCTACGAGTCCAAGGGGCTCATCAAGAGC
CGCCGGACGCCCGGAAACCAGCGCCGCTACACACGGGACACGCTGCGCCGCATCTCCGTC
ATCCGGGTGGCGCAGCGCATGGGCATCCCGCTGAGCACGGTCGCCGAGGCGCTGGCCGAG
CTGCCCGAGGAGCGCACGCCCACCCGGGAGGACTGGGCCCGGCTCTCGCGGCGCTGGCGC
GCGGACCTCAACGAGCGGATCGAGCAGCTGCTCGCGCTCCGCGACGGCCTGGAGGACTGC
ATCGGCTGTGGCTGTCTGTCGGTACGGGACTGCCCGCTGACCAATCCGTACGACGAGCTC
GGCGAGGAGGGTCCGGGGCCGCGCCGCCTGCTGCGCGAACCGACGGGCGCGGCGGCCCGC
GGCGGTGCCCGCGGCCGGGCGCGCACGGCCCGTACGGCGGAAAGGACCGCCCCCGTGGCC
GATCCGTGCGGGGGCGTGAGCCCGTCCTGCCAGGGCGGCGCGACCGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000551 Transcription regulator HTH, MerR (Domain)
[14-50] 4.3e-12 PF00376
PF00376   MerR
[14-25] 8.90000829418053e-12 PR00040 [25-38] 8.90000829418053e-12 PR00040 [48-68] 8.90000829418053e-12 PR00040
PR00040   HTHMERR
[16-38] PS00552
PS00552   HTH_MERR_1
[12-80] PS50937
PS50937   HTH_MERR_2
[13-81] 2.70000183580794e-29 SM00422
SM00422   HTH_MERR
IPR009061 DNA binding domain, putative (Domain)
[13-137] 2.19999900980708e-28 SSF46955
SSF46955   Putativ_DNA_bind
IPR010211 Redox-sensitive transcriptional activator SoxR (Family)
[12-151] 2.59999999999995e-68 TIGR01950
TIGR01950   SoxR
IPR015358 Transcription regulator MerR, DNA binding (Domain)
[55-120] 4.6e-16 PF09278
PF09278   MerR-DNA-bind
SignalP No significant hit
TMHMM No significant hit
Grana_00130 Grana_00130   Grana_00150 Grana_00150
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