Grana_00150 Grana_00150   Grana_00170 Grana_00170

Grana_00160 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction18,558 / 19,472 / + [in whole cluster]
18,558 / 19,472 / + [in contig]
Location18558..19472 [in whole cluster]
18558..19472 [in contig]
TypeCDS
Length915 bp (304 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-4 ketoreductase
Product (GenBank)putative dTDP4-keto-6-deoxyhexose reductase
Gene
Gene (GenBank)gra-orf22
EC number
Keyword
  • L-rhodinose
Note
Note (GenBank)
Reference
ACC
PmId
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
[11269719] Identification of four genes from the granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22 involved in the biosynthesis of L-rhodinose. (J Antibiot (Tokyo). , 2001)
comment
[PMID: 9831526](1998)
entire granaticin gene cluster(gra cluster)の報告。

ORF22: deoxyhexose生合成に関連したoxidoreductasesの可能性あり。

--
[PMID: 11269719](2001)
ORF22-25の各不活化mutantsは、dihydrogranaticin B産生ができなくなり、granaticinを蓄積する。
dihydrogranaticin Bはgranaticin Bの前駆体。
granaticin BはgranaticinにL-rhodinoseが追加された構造。
よって、ORF22-25はどれもL-rhodinose形成・付着に関連。
pathwayでの割り当ては配列相同性から。
Related Reference
ACC
O33708
NITE
Adria_00200
PmId
[9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997)
[10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999)
[9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998)
comment
6th(O33708) 36%, 7e-29
Streptomyces peucetius_dnmV
thymidine diphospho-4-keto-2,3,6-trideoxyhexulose reductase
[Adria_00200]NDP-hexose 4-ketoreductase

---
[PMID: 9209071](1997)
dnmV: 4-keto基の還元

dnmV不活化mutant産物解析と相補による回復から、daunosamine生合成に関与することは確認されている。dTDP-4-ketodeoxyhexulose ketoreductase機能の割り当ては配列解析に基づく。

---
[PMID: 10631513](1999)
異種性ホストにおけるL-daunosamineの生合成と付着のための最少の情報は、dnmLMJVUTS genesによってコードされる。
DnmVとavrE and eryBIV genesの産物は、DNR and DXRに由来する4'-epimeric anthracyclinesの合成を通して、dTDP-4-ketohexulose reductasesであることが証明されている(ref26)。

↓そのref26
[PMID: 9447597](1998) abstract
S.peucetius dnmV mutantに、Streptomyces avermitilis avrE or Saccharopolyspora eryBIV genesを異種性に導入すると、epirubicin (4'-epidoxorubicin) and 4'-epidaunorubicinを形成。

Saccharopolyspora eryBIV = dTDP-4-keto-6-deoxy-L-hexose 4-reductase

close this sectionSequence

selected fasta
>putative NDP-hexose C-4 ketoreductase [putative dTDP4-keto-6-deoxyhexose reductase]
MPHPTPAPRVLVLGGSGFVGRHVCAAFLARGWEVHGWARHDRGAPGVTTRAVDLVRAEPG
RLADELAAVAPDTVVNASGAVWGVTETEMADANQALVSRVLTALGAAPSPRFVQLGTVHE
HTAATAYGRTKAAATARVLAAGGTVLRLPNLLGPGTPEGSFLGRVGAQLARAARTGEAVT
VTTLAVRERREFLDVRDAADAVLAAAAPAAARRVRGRALDLATGCPVGVRELLDALIRIS
GVPARVEERLPDPGWAPPGGLEGVGAGAAGPDEAARLLGWRAVRGPEESLRGLWETAVSL
PAGL
selected fasta
>putative NDP-hexose C-4 ketoreductase [putative dTDP4-keto-6-deoxyhexose reductase]
ATGCCGCACCCCACCCCCGCGCCCCGCGTGCTCGTTCTCGGCGGCAGCGGTTTCGTCGGC
CGCCATGTGTGTGCCGCGTTCCTGGCACGGGGCTGGGAGGTGCACGGCTGGGCGAGGCAC
GACCGGGGGGCACCGGGCGTCACGACGCGCGCCGTCGACCTGGTGCGTGCCGAGCCCGGA
CGGCTCGCGGACGAGCTGGCCGCGGTGGCGCCGGACACGGTCGTCAACGCTTCCGGTGCC
GTGTGGGGCGTGACGGAGACGGAGATGGCGGACGCCAACCAGGCCCTCGTCTCCCGGGTC
CTCACGGCGCTCGGCGCCGCACCGTCGCCCCGTTTCGTCCAGCTCGGTACCGTGCACGAA
CACACGGCGGCGACCGCCTACGGACGGACCAAGGCGGCCGCCACCGCGCGGGTCCTGGCG
GCGGGCGGTACCGTGCTGCGGCTGCCGAACCTGCTGGGGCCCGGCACCCCCGAGGGCAGT
TTCCTGGGGCGCGTCGGCGCGCAGCTCGCCCGGGCCGCGCGCACCGGCGAGGCGGTGACC
GTGACGACGCTCGCCGTGCGCGAGCGCCGCGAGTTCCTGGACGTACGGGACGCCGCCGAC
GCGGTGCTCGCCGCGGCCGCCCCCGCCGCCGCCCGGCGCGTGCGGGGCCGGGCGCTCGAC
CTGGCGACCGGCTGCCCCGTCGGCGTACGGGAGCTGCTCGACGCGTTGATCCGGATAAGT
GGCGTGCCCGCGCGCGTGGAGGAGCGGCTCCCGGACCCGGGATGGGCACCTCCTGGTGGC
CTGGAGGGGGTGGGCGCCGGAGCGGCCGGTCCCGACGAGGCGGCGCGGCTGCTGGGCTGG
CGTGCGGTGCGCGGCCCCGAGGAGTCGCTGCGCGGACTGTGGGAGACCGCGGTGAGTCTT
CCAGCGGGCCTCTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[10-207] 1e-22 PF01370
PF01370   Epimerase
IPR016040 NAD(P)-binding domain (Domain)
[9-156] 6.1e-16 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-25] 0.384 Signal
Bacteria, Gram-positive   
[1-25] 0.897 Signal
Eukaryota   
TMHMM No significant hit
Grana_00150 Grana_00150   Grana_00170 Grana_00170
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