Jado_00080 Jado_00080   Jado_00100 Jado_00100

Jado_00090 : CDS information

close this sectionLocation

Organism
StrainISP5230 (=NBRC 13096)
Entry nameJadomycin B
Contig
Start / Stop / Direction9,859 / 11,073 / + [in whole cluster]
3,611 / 4,825 / + [in contig]
Location9859..11073 [in whole cluster]
3611..4825 [in contig]
TypeCDS
Length1,215 bp (404 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase chain length factor subunit
Product (GenBank)chain length determinant
GenejadB
Gene (GenBank)
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
[PMID:7881555](1994)
ORF2(=JadBに相当)は配列解析からtype II PKSのCLFであると提唱されている。
ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
jadBもKS-betaとして使用されている。

close this sectionPKS/NRPS Module

CLF4..362

close this sectionSequence

selected fasta
>polyketide synthase chain length factor subunit [chain length determinant]
MSASVVVTGLGVAAPNGLGREDFWASTLGGKSGIGPLTRFDPTGYPARLAGEVPGFAAEE
HLPSRLLPQTDRMTRLALVAADWALADAGVRPEEQDDFDMGVVTASASGGFEFGQGELQK
LWSQGSQYVSAYQSFAWFYAVNSGQISIRNGMKGPSGVVVSDQAGGLDAVAQARRQIRKG
TRLIVSGGVDASLCPWGWVAHVASDRLSTSEEPARGYLPFDREAQGHVPGEGGAILVMEA
AEAARERGARIYGEIAGYGSTFDPRPGSGREPGLRKAIELALADAGAAPGDIDVVFADAA
AVPELDRVEAEALNAVFGTGAVPVTAPKTMTGRLYSGAAPLDLAAAFLAMDEGVIPPTVN
VEPDAAYGLDLVVGGPRTAEVNTALVIARGHGGFNSAMVVRSAN
selected fasta
>polyketide synthase chain length factor subunit [chain length determinant]
ATGAGCGCGTCCGTGGTGGTGACCGGCCTCGGTGTCGCCGCACCCAACGGCCTGGGCCGC
GAGGACTTCTGGGCCTCGACCCTCGGCGGGAAGAGCGGCATCGGCCCGCTCACCCGCTTC
GACCCCACCGGCTACCCGGCGCGCCTCGCCGGCGAGGTGCCCGGCTTCGCCGCCGAGGAG
CACCTGCCGAGCCGGCTGCTGCCGCAGACCGACCGGATGACCCGCCTCGCGCTGGTCGCC
GCGGACTGGGCGCTGGCCGACGCGGGCGTCCGCCCCGAGGAGCAGGACGACTTCGACATG
GGCGTGGTCACGGCCAGCGCCTCCGGCGGCTTCGAGTTCGGCCAGGGCGAGCTGCAGAAG
CTGTGGAGCCAGGGCAGCCAGTACGTCAGCGCGTACCAGTCCTTCGCCTGGTTCTACGCC
GTCAACAGCGGCCAGATCTCCATCCGCAACGGCATGAAGGGCCCCAGCGGCGTGGTCGTC
AGCGACCAGGCCGGCGGTCTGGACGCCGTCGCGCAGGCCCGCCGACAGATCCGCAAGGGC
ACCCGGCTCATCGTCTCCGGTGGCGTCGACGCCTCGCTCTGCCCCTGGGGCTGGGTGGCG
CACGTCGCCTCCGACCGGCTCAGCACCAGCGAGGAGCCCGCCCGGGGCTACCTGCCCTTC
GACCGCGAAGCCCAGGGCCACGTGCCCGGCGAGGGTGGGGCGATCCTCGTCATGGAGGCC
GCCGAGGCGGCCCGGGAGCGCGGGGCCCGGATCTACGGCGAGATCGCCGGATACGGCTCC
ACCTTCGACCCCCGGCCGGGCAGCGGGCGCGAGCCCGGCCTGCGCAAGGCCATCGAACTC
GCCCTGGCCGACGCCGGAGCGGCACCCGGTGACATCGACGTCGTCTTCGCGGACGCGGCC
GCCGTCCCCGAGCTCGACCGCGTCGAGGCCGAGGCCCTCAACGCCGTCTTCGGCACCGGC
GCCGTCCCGGTCACCGCGCCCAAGACGATGACCGGCCGGCTGTACTCCGGCGCCGCGCCG
CTCGACCTGGCCGCCGCGTTCCTCGCGATGGACGAGGGCGTCATCCCGCCCACCGTCAAC
GTCGAGCCGGACGCCGCGTACGGCCTCGACCTGGTCGTCGGCGGGCCCCGTACCGCCGAG
GTGAACACCGCCCTGGTGATCGCCCGCGGTCACGGCGGCTTCAACTCCGCGATGGTCGTC
CGTTCCGCGAACTGA
CLF4..362
CLF10..1086

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[4-243] 5.69999999999998e-58 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[252-362] 4.29999999999998e-28 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[3-257] 1e-57 G3DSA:3.40.47.10 [269-403] 7.00000000000005e-37 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-249] 3.59998852907783e-62 SSF53901 [212-403] 5.7999965385478e-46 SSF53901
SSF53901   Thiolase-like
SignalP
[1-19] 0.877 Signal
Eukaryota   
[1-25] 0.244 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Jado_00080 Jado_00080   Jado_00100 Jado_00100
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