Jado_00180 : CDS information

Location

Organism	Streptomyces venezuelae
Strain	ISP5230 (=NBRC 13096)
Entry name	Jadomycin B
Contig	AF222693
Start / Stop / Direction	17,305 / 18,093 / + [in whole cluster] 122 / 910 / + [in contig]
Location	17305..18093 [in whole cluster] 122..910 [in contig]
Type	CDS
Length	789 bp (262 aa)

Annotation

Category	1.4 Other
Product	putative phosphopantetheinyl transferase
Product (GenBank)	phosphopantetheinyl transferase
Gene
Gene (GenBank)	jadM
EC number
Keyword
Note
Note (GenBank)	similar to HetI
Reference	ACC Q9L7M4 PmId [11390684] Cloning and functional analysis of a phosphopantetheinyl transferase superfamily gene associated with jadomycin biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 2001) comment jadomycin B 生産菌Streptomyces venezuelae ISP5230にあるjadMをE.coliにて発現、クローニング。 jadM欠損株は、jadomycin生産がwild-typeの2-5%程度に落ちた。また、この欠損株の生育は良く、chloramphenicolの生産には変化がなかったことから、jadMがコードしているholo-ACP synthaseはjadomycin B合成の初期段階に関与しているのではないかと言っている。 holo-ACP synthaseであるというのは配列解析(その他PPTaseとのalignment、系統樹）から。
Related Reference	ACC Q9RFL1 NITE Myxo_00010 PmId [10601310] New lessons for combinatorial biosynthesis from myxobacteria. The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1. (J Biol Chem. , 1999) [11134965] The mtaA gene of the myxothiazol biosynthetic gene cluster from Stigmatella aurantiaca DW4/3-1 encodes a phosphopantetheinyl transferase that activates polyketide synthases and polypeptide synthetases. (J Biochem. , 2001) comment Blast 42nd, id32%, 2e-15 Stigmatella aurantiaca_mtaA MtaA [Myxo_00010]phosphopantetheinyl transferase -- [PMID: 10601310](1999) Myxothiazol生合成gene clusterの同定論文。 MtaA: Ppant transferase Ppant=4'-phosphopantetheinyl 4'-phosphopantetheinyl transferase MtaAによってmyxothiazol合成は活性化される。 mtaAへのkanamycin resistance geneの挿入はmyxothiazol合成を阻害する。 MtaB-MtaGのACP, PCP domainは4'-phosphopantetheine cofactorのためのputative binding siteのProsite consensus signatureを含んでいる。 -- [PMID: 11134965](2001) MtaAをACP, PCPと異種性発現して、4'-phosphopantetheinyl transferases機能があることを確認。 MtaAは他種のACP, PCPも同様に加工でき、基質特異性がゆるい。
Family/Domain	FD IPR008278 comment [IPR008278] 4'-phosphopantetheinyl transferase (Domain) function; magnesium ion binding, holo-[acyl-carrier-protein] synthase activity

Sequence

>putative phosphopantetheinyl transferase [phosphopantetheinyl transferase]
MPLLRVADDELHLWTLRPPPPGSAAADALATGELDGAERRRAEAYVRPRDRVQYLAAHLA
LRRVLARYTGVPAGRLRFGREAAGRPVLLGVAGPPSFSLSHSHGLVLLGVARRPVGVDVQ
RVPPYATAEVCRGALHPAEREELAALPEDRLAAAFARLWTRKEAYLKGLGTGLARGLATD
FLGTPGHGGVRRPAGWTVLDLPGGPDHAAAAALHGDTAPRVTTRALRPEDLTSTTHASHD
TQTPHDTYDPYDTQTERGVGSA

>putative phosphopantetheinyl transferase [phosphopantetheinyl transferase]
ATGCCGCTCCTGAGGGTCGCGGACGATGAGCTGCACCTCTGGACCCTCCGCCCGCCCCCG
CCGGGGTCCGCGGCCGCCGACGCCCTCGCGACCGGGGAACTCGACGGTGCCGAGCGGCGC
CGGGCGGAGGCGTACGTACGCCCCCGGGACCGGGTGCAGTACCTCGCGGCGCACCTCGCG
CTGCGCCGGGTCCTCGCCCGCTACACCGGAGTCCCGGCCGGGCGGCTGCGCTTCGGCCGT
GAGGCCGCCGGGCGGCCCGTGCTGCTCGGGGTGGCGGGACCGCCGTCCTTCTCGCTGTCG
CACAGCCACGGGCTGGTGCTGCTCGGGGTGGCCCGGCGGCCGGTGGGCGTCGACGTCCAG
CGCGTCCCGCCGTACGCCACCGCGGAGGTGTGCCGCGGCGCGCTCCACCCGGCCGAGCGG
GAGGAGCTCGCGGCCCTGCCGGAGGACCGTCTCGCGGCGGCCTTCGCCCGGCTGTGGACC
CGGAAGGAGGCCTACCTCAAGGGACTCGGCACGGGACTCGCGCGGGGCCTCGCCACCGAC
TTCCTCGGCACGCCGGGCCACGGCGGCGTCCGGCGGCCCGCCGGCTGGACGGTCCTCGAC
CTGCCGGGCGGACCCGACCACGCGGCGGCCGCCGCCCTGCACGGCGACACCGCGCCACGG
GTCACGACGAGGGCCCTGCGCCCCGAGGACCTGACCAGCACCACACACGCATCGCACGAC
ACGCAGACACCGCACGACACGTACGACCCGTACGACACGCAGACCGAAAGGGGAGTGGGA
AGCGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR008278 4'-phosphopantetheinyl transferase (Domain) SSF56214 4'-phosphopantetheinyl transferase G3DSA:3.90.470.20 no description PF01648 ACPS
SignalP	Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	No significant hit

Jado_00150 Jado_00190