Medem_00350 : CDS information

Location

Organism	Streptomyces sp.
Strain	AM-7161
Entry name	Medermycin
Contig	AB103463
Start / Stop / Direction	35,374 / 34,145 / - [in whole cluster] 35,374 / 34,145 / - [in contig]
Location	complement(34145..35374) [in whole cluster] complement(34145..35374) [in contig]
Type	CDS
Length	1,230 bp (409 aa)

Annotation

Category
Product
Product (GenBank)	putative secreted chitosanase
Gene
Gene (GenBank)	med-ORF4
EC number
Keyword
Note	It is supposed that this ORF is not involved in medermycin biosynthesis.
Note (GenBank)
Reference	ACC Q7WT07 PmId [12855716] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters. (Microbiology. , 2003) comment med-ORF4: No relation?

Sequence

>[putative secreted chitosanase]
MPPTINGCAAAASRPSSPPRPPASCSPPLAGLTSATAAAVNLAANRPVTTSSTESGSFGG
AKAVAGSASTRWASAEGVDNQWIRIDLGSATTLGRVVLKWEAAYARAYRVELSDDGSTWR
QIHATTTGDGGTDDLSVNGTGRYLRVFGTQRGTPYGYSLYEVEAYGAGATTPPPSSGTGL
DDPRKKEVAMKLVSSFENSSLDWRAQFAYIEDIDDGRGYTAGIIGFCSGTGDMLELVERY
TAKKPGNPLARYLGALRAVDGTDSHEGLDPGFTNAWRQAAQDTVFQQTQEEERDRVYFTP
AVSQAKQDGLRALGQFAYYDAAVMHGESGFRDIRSVALSRALPPSRGGDEKAYLKAFLDA
REEEMRKEEAHSDTTRVSTAQRRFLNEGNLDLNTPLHWSVYGEAFSLTG

>[putative secreted chitosanase]
ATGCCCCCCACGATCAACGGCTGCGCGGCCGCCGCCTCGCGGCCCTCGTCGCCGCCGCGG
CCACCGGCGTCCTGCTCCCCACCCCTGGCCGGCCTCACGTCCGCCACCGCCGCGGCCGTC
AACCTCGCCGCGAACCGCCCCGTCACCACCTCCTCCACCGAGTCCGGCTCGTTCGGCGGC
GCCAAGGCCGTGGCAGGCTCCGCCTCCACCCGCTGGGCAAGCGCCGAAGGCGTCGACAAC
CAGTGGATCCGGATAGACCTCGGCTCGGCGACGACCCTCGGCCGGGTCGTCCTCAAGTGG
GAGGCGGCCTATGCCAGGGCGTACCGCGTCGAGCTCTCGGACGACGGCTCGACCTGGCGC
CAGATCCACGCCACCACCACCGGCGACGGCGGCACCGACGACCTGAGCGTGAACGGAACC
GGCCGCTACCTCCGCGTCTTCGGGACGCAGCGCGGCACGCCGTACGGCTACTCGCTCTAC
GAGGTCGAGGCGTACGGCGCGGGCGCGACGACCCCGCCCCCGTCCTCCGGCACCGGCCTC
GACGACCCGCGCAAGAAGGAGGTCGCCATGAAGCTGGTGTCGTCCTTCGAGAACTCCTCC
CTCGACTGGCGCGCCCAGTTCGCGTACATCGAGGACATCGACGACGGGCGCGGCTACACC
GCCGGCATCATCGGCTTCTGCTCGGGCACGGGTGACATGCTGGAGCTGGTCGAGCGCTAC
ACGGCGAAGAAGCCGGGCAACCCGCTCGCCCGCTACCTCGGCGCCCTGCGCGCGGTGGAC
GGCACCGACTCCCACGAGGGCCTGGACCCGGGCTTCACGAACGCCTGGCGCCAGGCGGCG
CAGGACACCGTGTTCCAGCAGACCCAGGAGGAGGAGCGCGACCGCGTCTACTTCACTCCC
GCGGTCTCCCAGGCCAAGCAGGACGGCCTGCGCGCGCTGGGGCAGTTCGCCTACTACGAC
GCCGCCGTCATGCACGGCGAGTCCGGCTTCCGCGACATCCGCTCGGTCGCCCTCTCCCGC
GCCCTGCCGCCGTCCCGGGGCGGAGACGAGAAGGCCTATCTGAAGGCCTTCCTCGACGCG
CGTGAGGAGGAGATGCGCAAGGAGGAGGCGCACAGCGACACCACCCGGGTCAGCACGGCC
CAGCGCCGCTTCCTGAACGAGGGCAACCTCGACCTGAACACCCCGCTCCACTGGAGCGTC
TACGGCGAGGCCTTCTCCCTCACCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000400 Glycoside hydrolase, family 46 (Family) PF01374 Glyco_hydro_46 IPR000421 Coagulation factor 5/8 C-terminal type domain (Domain) SM00231 FA58C PF00754 F5_F8_type_C PS50022 FA58C_3 IPR008979 Galactose-binding domain-like (Domain) SSF49785 Gal_bind_like IPR023099 Glycoside hydrolase, family 46, N-terminal (Domain) G3DSA:3.30.386.10 Glyco_hydro_46_N IPR023346 Lysozyme-like domain (Domain) SSF53955 SSF53955
SignalP	Bacteria, Gram-negative Eukaryota Bacteria, Gram-positive
TMHMM	No significant hit

Medem_00340 Medem_00360