Naphcy_00040 : CDS information

Location

Organism	Streptomyces arenae
Strain	DSM 40737
Entry name	Naphthocyclinone
Contig	AF098965
Start / Stop / Direction	9,752 / 8,805 / - [in whole cluster] 4,289 / 5,236 / + [in contig]
Location	complement(8805..9752) [in whole cluster] 4289..5236 [in contig]
Type	CDS
Length	948 bp (315 aa)

Annotation

Category	3.4 other modification
Product	cyclase/dehydratase
Product (GenBank)	aromatase
Gene
Gene (GenBank)	ncnD
EC number
Keyword	1st ring
Note
Note (GenBank)
Reference	ACC Q9X464 PmId [10206788] Isolation and characterization of the naphthocyclinone gene cluster from Streptomyces arenae DSM 40737 and heterologous expression of the polyketide synthase genes. (Gene. , 1999) comment S. coelicolorのactinorhodin PKS由来保存領域でのハイブリによって、putative naphthocyclinone gene clusterを含むcosmid cloneを分離。 NcnD: aromatase act PKS + act KR + ncnD →SEK34産生 act PKS + act KR + act ARO →SEK34産生 tcm PKS + act KR　→RM20b,c産生 tcm PKS + act KR + ncnD →RM20b aromatized formであるP517産生よって、NcnDはC-16骨格だけでなく、C-20 coresでも働き、 S.arenae aromataseはact aromataseより広い基質幅を持つ。

Sequence

>cyclase/dehydratase [aromatase]
MTAGPRPLHAEHTRVVHAPPGPLYDLVADVTLWPAVFGPTVHARRLEGGPDSERIRLWAT
VNGRVSDWVSRRTLDPGRFTVGFEQEVSSAPVASMGGEWLFRELGGGRTEVVLRHHFTVV
DDDPAAVSWVQEALDRNSAAELGALARVVETGHPPREIVFSFTDTLALTGPAESAYEFVA
RADLWPERLPHVAGVTLTETAPGVQDLRMETATADGSTHTTHSVRVCRRPEWIAYKQLRT
PELLLGHSGLWTFGEGPDGPFATASHTVALNPEAVSRVLGADRTTADARRHVRELLGRNS
RTTLAHAAGQEAPAR

>cyclase/dehydratase [aromatase]
GTGACGGCCGGACCCCGCCCGCTGCACGCCGAGCACACCCGGGTGGTGCACGCGCCGCCC
GGTCCGCTGTACGACCTGGTGGCGGACGTGACGCTGTGGCCGGCCGTCTTCGGCCCCACC
GTGCACGCCCGCCGGCTGGAAGGCGGCCCGGACAGCGAGCGGATCCGGCTCTGGGCGACG
GTGAACGGCCGGGTGAGCGACTGGGTGTCGCGCCGCACCCTCGATCCGGGCCGGTTCACG
GTCGGCTTCGAGCAGGAGGTCAGCAGTGCGCCGGTCGCCTCGATGGGCGGCGAGTGGCTC
TTCCGTGAGCTGGGCGGCGGCCGGACCGAGGTCGTCCTGCGGCACCACTTCACGGTCGTC
GACGACGACCCGGCCGCCGTGTCCTGGGTGCAGGAGGCGCTGGACCGCAACAGCGCGGCG
GAACTCGGCGCGCTGGCCCGGGTCGTGGAGACCGGGCACCCGCCGCGGGAGATCGTGTTC
TCGTTCACCGACACCCTCGCGCTGACCGGCCCGGCGGAGTCGGCCTACGAGTTCGTCGCC
CGGGCCGACCTGTGGCCGGAGCGGCTTCCGCACGTGGCCGGGGTGACCCTCACCGAGACC
GCGCCCGGGGTGCAGGACCTGCGGATGGAGACGGCGACGGCGGACGGCTCGACCCACACC
ACCCATTCGGTACGGGTGTGCCGGCGGCCGGAGTGGATCGCGTACAAGCAGCTCCGTACC
CCGGAGCTGCTGCTCGGGCACAGCGGGCTGTGGACGTTCGGCGAGGGACCGGACGGACCG
TTCGCCACCGCGAGCCACACGGTGGCCCTGAACCCGGAGGCGGTGTCCCGGGTGCTGGGG
GCGGACCGCACGACCGCGGACGCCCGACGGCACGTGCGGGAACTGCTCGGCCGCAACAGC
CGCACCACGCTGGCGCACGCGGCCGGACAGGAGGCACCGGCCCGGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005031 Streptomyces cyclase/dehydrase (Family) PF03364 Polyketide_cyc IPR019587 Polyketide cyclase/dehydrase (Family) PF10604 Polyketide_cyc2 IPR023393 START-like domain (Domain) G3DSA:3.30.530.20 G3DSA:3.30.530.20
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Naphcy_00090 Naphcy_00030