Naphcy_00070 Naphcy_00070   Naphcy_00090 Naphcy_00090

Naphcy_00080 : CDS information

close this sectionLocation

Organism
StrainDSM 40737
Entry nameNaphthocyclinone
Contig
Start / Stop / Direction4,698 / 5,561 / + [in whole cluster]
717 / 1,580 / + [in contig]
Location4698..5561 [in whole cluster]
717..1580 [in contig]
TypeCDS
Length864 bp (287 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)Ncn-orf2
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11816965] Heterologous expression of the naphthocyclinone hydroxylase gene from Streptomyces arenae for production of novel hybrid polyketides. (Antonie Van Leeuwenhoek. , 2001)
comment
naphthocyclinone生合成minimal PKS genesの約9kbp下流にある3.5kb fragmentの解析報告。

このfragmentに含まれるncn-ORF2の推定peptide配列は、actVA-3、gra-ORF28、gra-ORF30の推定タンパクに似ている。
Related Reference
ACC
Q93IZ2
NITE
Actino_00090
PmId
[1766437] Organisation and functions of the actVA region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor. (Mol Gen Genet. , 1991)
comment
Blast 5th, id42%, 2e-31
Streptomyces coelicolor_SCO5078
Putative uncharacterized protein

actinorhodin biosynthetic gene clusterのactVA領域の配列解析。

actVA-ORF3とactVA-ORF5(たぶんactVA-ORF4も)は、act mutantsの相補実験からC-6 hydroxylationに関与するとされている。ただ、ここで使用しているactVA-ORF3のN末は欠損していて、実験的に微妙。

actVA-ORF1-5を含むpIJ2315をmedermycin産生株に導入するとhybrid化合物mederrhodin A(8-hydroxymedermycin)を産生するので、C-8 hydroxylationへの関与についても述べられている。

close this sectionSequence

selected fasta
>hypothetical protein [Ncn-orf2]
MTYTRHASDTGFRRTGGRSRCSPTSSGSRMPAWQPDGLPPGWLARPAAGLPAGDGTARVT
PEGLVVAPPGTDPAHPRTPVRRPAPSAPLGHLRWAAFAGTATPPGFTLPDTGLLTAHMEL
AAEVYGPGADRPTAAGIADTRHGMGGMLCVDLGSGLVLDFLLTNDRVWVLYERLGRPGAG
HGTFTYEVPVAERSPGRRHRCAIRVDRARGTAVWLLDGTEVFAVPDLGRRLPSRAAHITR
SADGPEESVRPGRLALGIGLFGELAEGSGVRLTVRRATVTRSTPGTS
selected fasta
>hypothetical protein [Ncn-orf2]
GTGACGTACACACGACATGCCAGTGATACGGGATTCCGGCGGACGGGCGGGAGGAGCCGG
TGTTCGCCGACGAGTTCCGGCTCCCGGATGCCGGCCTGGCAGCCGGACGGGCTGCCCCCG
GGATGGCTGGCCCGTCCCGCCGCCGGGCTGCCCGCCGGTGACGGGACCGCCCGCGTCACC
CCGGAGGGCCTGGTGGTGGCACCCCCGGGCACGGACCCGGCCCACCCGCGAACCCCGGTT
CGCCGTCCCGCCCCGTCGGCGCCGCTGGGCCACCTGCGCTGGGCCGCCTTCGCCGGCACC
GCCACCCCACCCGGCTTCACCCTGCCCGACACCGGACTCCTCACCGCCCACATGGAGCTG
GCCGCCGAGGTGTACGGCCCGGGTGCGGACCGTCCGACCGCGGCCGGCATCGCCGACACC
CGGCACGGGATGGGCGGGATGCTCTGTGTGGATCTGGGGTCCGGGCTGGTGCTCGACTTC
CTGCTGACCAATGACCGGGTCTGGGTGCTGTACGAGCGGCTCGGCCGGCCCGGCGCCGGT
CACGGCACGTTCACGTACGAGGTCCCGGTCGCCGAGCGGTCGCCTGGCCGTCGGCACCGG
TGTGCGATACGCGTCGACCGGGCCCGCGGCACCGCGGTGTGGCTGCTGGACGGCACGGAG
GTGTTCGCCGTGCCGGACCTCGGCCGTCGCCTCCCGTCCCGCGCCGCCCACATCACCCGC
TCCGCCGACGGCCCCGAGGAGAGCGTCCGGCCCGGCCGGCTCGCCCTCGGCATCGGCCTG
TTCGGCGAACTCGCGGAAGGGTCCGGGGTACGCCTCACGGTCCGCCGGGCCACCGTGACA
CGCTCCACCCCCGGCACGTCGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro No significant hit
SignalP No significant hit
TMHMM No significant hit
Naphcy_00070 Naphcy_00070   Naphcy_00090 Naphcy_00090
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