Nogl_00200 Nogl_00200   Nogl_00220 Nogl_00220

Nogl_00210 : CDS information

close this sectionLocation

Organism
StrainATCC 27451 (=NBRC 13445)
Entry nameNogalamycin
Contig
Start / Stop / Direction20,406 / 21,458 / + [in whole cluster]
7,769 / 8,821 / + [in contig]
Location20406..21458 [in whole cluster]
7769..8821 [in contig]
TypeCDS
Length1,053 bp (350 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative dTDP-glucose 4,6-dehydratase
Product (GenBank)putative dTDP-glucose-4,6-dehydratase
Gene
Gene (GenBank)snogK
EC number4.2.1.46
Keyword
  • nogalamine
  • nogalose
Note
Note (GenBank)
Reference
ACC
PmId
[11683270] The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures. (Mol Genet Genomics. , 2001)
comment
S. nogalater genomic DNA fragmentの配列解析と、異種性発現による産物解析。
nogalamycin生合成に関する20ORFsが明らかになった。

snogK: dTDP-glucose-4,6-dehydratase

配列解析より、deoxyhexose生合成で2つめのstepを触媒すると割り当て。
Related Reference
ACC
Q194R3
NITE
Mith_00070
PmId
[9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997)
comment
Blast 4th, id69%, 1e-123
Streptomyces argillaceus_mtmE
dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)

E. coli発現タンパクで活性見ている。
mtmD: glucose-1-phosphate:dTTP thymidylyl transferase
mtmE: dTDP-D-glucose 4,6-dehydratase

close this sectionSequence

selected fasta
>putative dTDP-glucose 4,6-dehydratase [putative dTDP-glucose-4,6-dehydratase]
MASHTSATTDVNILVTGAVGFIGSAYVRMLLENRAPGAGAPAVRVTVLDKLTYAGNLTNL
DAVRGDRLRFVRGDILDAELVDELMAHSDQVVHFAAESHVDRSIRAADDFVLTNVVGTQR
LLDAALRHGVEPFVLVSTDEVYGSIASGSWPEEHPLSPNSPYAASKASADLMAFACHRTH
GLDVRVTRCSNNYGPRQHPEKLIPRFVTNLLDGLPVPLYGDGRNVREWLHVEDHCRGVDL
VRTAGRPGGVYHIGGGRELSNRELVGMLLELCGADWSSVRHVPDRKGHDLRYSLDWGRAR
EELGYRPAREFSSGLRSTVQWYRENRSWWEPLKRGVTAPGGTSTVVPGVR
selected fasta
>putative dTDP-glucose 4,6-dehydratase [putative dTDP-glucose-4,6-dehydratase]
ATGGCGTCGCACACCTCCGCGACGACGGACGTGAACATCCTGGTCACCGGCGCGGTCGGT
TTCATCGGCTCGGCCTATGTGCGCATGCTGCTGGAGAACCGGGCCCCCGGAGCGGGGGCC
CCGGCGGTACGCGTCACCGTCCTCGACAAACTGACGTACGCGGGCAACCTCACGAACCTG
GACGCGGTACGCGGTGACCGGCTGCGCTTCGTGCGGGGCGACATCCTCGACGCCGAGCTG
GTCGACGAGCTGATGGCACACAGCGACCAGGTCGTCCACTTCGCCGCGGAGTCGCACGTC
GACCGGTCCATCCGCGCGGCCGACGACTTCGTGCTGACCAACGTCGTCGGCACCCAGCGG
CTGCTGGACGCCGCGCTGCGCCACGGCGTGGAGCCCTTCGTCCTGGTGTCCACGGACGAG
GTCTACGGCTCCATCGCGTCCGGTTCCTGGCCGGAGGAGCACCCGCTGAGCCCCAACTCC
CCGTACGCCGCCTCCAAGGCGTCGGCCGACCTGATGGCGTTCGCCTGCCACCGCACGCAC
GGCCTGGACGTGCGGGTGACCCGCTGTTCCAACAACTACGGCCCGCGTCAGCACCCGGAG
AAGCTCATCCCGCGCTTCGTCACCAACTTGTTGGACGGGCTGCCGGTGCCGCTGTACGGG
GACGGGCGGAACGTGCGGGAGTGGCTGCACGTGGAGGACCACTGCCGGGGCGTGGACCTG
GTGCGCACCGCGGGCCGGCCCGGCGGTGTGTACCACATCGGCGGCGGCCGGGAGCTCTCC
AACCGGGAGCTGGTGGGTATGTTGCTGGAGCTGTGCGGGGCGGACTGGAGCAGCGTCCGG
CACGTGCCCGACCGCAAGGGGCACGACCTGCGTTACAGCCTGGACTGGGGCAGGGCCCGT
GAGGAACTGGGCTACCGTCCGGCGCGGGAGTTCTCCTCGGGGCTGCGCTCGACGGTCCAG
TGGTACCGCGAGAACCGGTCCTGGTGGGAGCCGCTCAAGCGGGGAGTCACGGCGCCGGGC
GGTACGTCCACCGTCGTCCCCGGGGTCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[13-254] 1.29999999999998e-63 PF01370
PF01370   Epimerase
IPR005888 dTDP-glucose 4,6-dehydratase (Family)
[12-332] 3.99999999999998e-120 TIGR01181
TIGR01181   DTDP_gluc_dehyt
IPR016040 NAD(P)-binding domain (Domain)
[12-210] 1.40000000000001e-64 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM
[13-30] Transmembrane (i-o)
Transmembrane 1   
Nogl_00200 Nogl_00200   Nogl_00220 Nogl_00220
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