Polk_00090 Polk_00090   Polk_00110 Polk_00110

Polk_00100 : CDS information

close this sectionLocation

Organism
StrainTü6028
Entry namePolyketomycin
Contig
Start / Stop / Direction11,296 / 12,594 / + [in whole cluster]
11,296 / 12,594 / + [in contig]
Location11296..12594 [in whole cluster]
11296..12594 [in contig]
TypeCDS
Length1,299 bp (432 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative pyridoxamine 5'-phosphate-dependent NDP-hexose C-3 dehydratase
Product (GenBank)PokS5
Gene
Gene (GenBank)pokS5
EC number
Keyword
  • beta-D-amicetose
Note
Note (GenBank)
  • putative NDP-hexose 3-dehydratase
Reference
ACC
PmId
[19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009)
comment
polyketomycin生合成clusterの解析論文。

機能推定は配列解析から。
pokS5: NDP-hexose 3-dehydratase
Related Reference
ACC
Q9ALN8
NITE
Spino_00050
PmId
[17076492] Characterization of SpnQ from the spinosyn biosynthetic pathway of Saccharopolyspora spinosa: mechanistic and evolutionary implications for C-3 deoxygenation in deoxysugar biosynthesis. (J Am Chem Soc. , 2006)
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
Blast 21st, id70%, 1e-167
Saccharopolyspora spinosa_spnQ
probable NDP-hexose-3,4-dehydratase
[DoBISCUIT]pyridoxamine 5'-phosphate-dependent NDP-hexose C-3 dehydratase

--
[PMID: 17076492]
SpnQをクローニング、過剰発現、精製して、"ColD-like" conditions と "E1-like" conditionsの両方で触媒活性をテスト。
E3のような特異的reductaseを必要とせず、一般的なferredoxin/ferredoxin reductase またはflavodoxin/flavodoxin reductaseと効率的に働く。

--
[PMID: 18345667]
dTDP-D-forosamine形成に関連する5つの酵素SpnO, N, Q, R, and Sの生化学的機能解明。
SpnQのsteady-state kinetic parameters、基質特異性を調査。

close this sectionSequence

selected fasta
>putative pyridoxamine 5'-phosphate-dependent NDP-hexose C-3 dehydratase [PokS5]
MDIRSALLELTRKFHQEQTEDSFVPGQTAILTSGAVLDEEDRVAFVEAALDMTIAAGARA
RTFESRFARAMKARKAHLTNSGSSANLLALSALTSPQLEDARLVPGDEVITVATGFPTTV
NPVLQNGLVPVFVDIELGTYNTTLERVEEAIGPRTRAIMVAHALGNPFPAAEIAELASRH
GLFLVEDNCDAVGSLYQGRLTGTFGDLSTVSFYPAHHLTMGEGGCVLTGNLALARIVESM
RDWGRDCWCEPGEDNRCLRRFDYSFGTLPQGYDHKYVFSHVGYNLKTTDVQAALGLSQLR
RLEEFGAARRRNWQRLRDGLQDVPGLLLPEATPGSDPSWFGFVLTVLPDAPFTRTALVSF
LEDRKIRTRRLFAGNLTRHPAYEGRHFRVVGELTNSDVTTEATFWIGVYPGITPEMVDYM
VASIREFVAAAS
selected fasta
>putative pyridoxamine 5'-phosphate-dependent NDP-hexose C-3 dehydratase [PokS5]
ATGGACATACGCAGCGCACTGCTGGAACTGACCCGGAAATTCCACCAGGAGCAGACCGAG
GACAGCTTCGTCCCCGGACAGACGGCGATCCTCACCTCGGGCGCGGTGCTGGACGAGGAG
GACCGGGTCGCCTTCGTCGAAGCGGCCCTGGACATGACGATCGCCGCCGGAGCGCGGGCC
CGGACCTTCGAGAGCCGGTTCGCCCGCGCGATGAAGGCGCGCAAGGCACATCTCACCAAC
TCCGGTTCCTCGGCCAACCTCCTCGCGCTCAGCGCCCTCACCTCGCCGCAGCTGGAGGAT
GCCCGGCTGGTCCCCGGCGACGAGGTGATCACGGTCGCGACGGGCTTCCCCACGACCGTC
AACCCGGTGCTGCAGAACGGCCTCGTACCGGTCTTCGTCGACATCGAACTCGGCACGTAC
AACACGACGCTGGAGCGCGTCGAGGAGGCCATCGGGCCGCGGACCCGCGCCATCATGGTC
GCGCACGCGCTCGGCAACCCCTTCCCGGCGGCCGAGATCGCCGAACTCGCCTCCCGGCAC
GGCCTGTTCCTCGTCGAGGACAACTGCGACGCCGTCGGCTCGCTCTACCAGGGCCGGCTC
ACCGGCACCTTCGGCGACCTGTCCACCGTCAGCTTCTATCCCGCCCACCACCTCACGATG
GGGGAGGGCGGCTGCGTACTGACCGGGAATCTGGCGCTCGCGCGGATCGTCGAGTCGATG
CGGGACTGGGGACGCGACTGCTGGTGCGAACCGGGCGAGGACAACCGCTGCCTGCGCCGC
TTCGACTACAGCTTCGGCACGCTCCCGCAGGGATACGACCACAAGTACGTGTTCTCGCAC
GTCGGCTACAACCTCAAGACGACGGACGTCCAGGCGGCCCTCGGCCTGAGCCAGCTGCGC
CGCCTGGAGGAGTTCGGCGCGGCACGGCGGCGCAACTGGCAGCGCCTGCGCGACGGGCTC
CAGGACGTCCCCGGGCTGCTGCTGCCCGAGGCGACGCCCGGCAGCGATCCCAGCTGGTTC
GGCTTCGTCCTCACCGTCCTGCCCGACGCCCCCTTCACCCGCACCGCCCTGGTGTCCTTC
CTGGAGGACCGCAAGATCCGCACCCGGAGGCTGTTCGCGGGCAACCTCACCCGGCATCCC
GCTTACGAGGGACGGCACTTCCGGGTGGTGGGGGAGCTGACCAACAGCGACGTCACCACC
GAGGCGACGTTCTGGATCGGCGTCTACCCCGGCATCACCCCGGAGATGGTGGACTACATG
GTCGCCTCGATACGCGAGTTCGTGGCGGCGGCGTCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
[39-425] 9.2000000000001e-97 PF01041
PF01041   DegT_DnrJ_EryC1
[1-432] 1.90000694315261e-110 PIRSF000390
PIRSF000390   PLP_StrS
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[34-301] 2.39999999999997e-69 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[302-425] 3.09999999999998e-47 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[38-431] 6.29998179434741e-91 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit
Polk_00090 Polk_00090   Polk_00110 Polk_00110
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