Polk_00420 : CDS information

Location

Organism	Streptomyces diastatochromogenes
Strain	Tü6028
Entry name	Polyketomycin
Contig	FJ483966
Start / Stop / Direction	50,634 / 51,608 / + [in whole cluster] 50,634 / 51,608 / + [in contig]
Location	50634..51608 [in whole cluster] 50634..51608 [in contig]
Type	CDS
Length	975 bp (324 aa)

Annotation

Category	5.1 general function
Product	putative acyl-CoA dehydrogenase
Product (GenBank)	PokU3
Gene
Gene (GenBank)	pokU3
EC number	1.3.99.-
Keyword
Note
Note (GenBank)	acyl-CoA dehydrogenase
Reference	ACC C0JWD7 PmId [19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009) comment polyketomycin生合成clusterの解析論文。機能推定は配列解析から。 pokU3: acyl-CoA dehydrogenase
Family/Domain	FD IPR013786, IPR006091, IPR009075 comment [IPR013786] Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain) 現在はIPR006092が削除され、このentryがPF02771を含む。 Molecular Function GO:0050660 flavin adenine dinucleotide binding GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors --- [IPR006091] Acyl-CoA oxidase/dehydrogenase, central domain (Domain) Molecular Function GO:0003995 acyl-CoA dehydrogenase activity --- [IPR009075] Acyl-CoA dehydrogenase/oxidase C-terminal (Domain) 現在はIPR006090が削除され、このentryがPF00441を含む。 Molecular Function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors

Sequence

>putative acyl-CoA dehydrogenase [PokU3]
MHLEYTPDQQRLRTELRDYFAQLVPDNAYARYADPASQKRFYRETVRRLGADGWLGVGWP
KEYGGRGLTPMEQFIFFDEAAQAGVPLPLMALNTVGPTIMQFGTEEQKSHFLPRILSGEI
DFAIGYSEPDAGTDLAALKTKAVRDGDGDYVVNGQKIWTTNGDTADWVWLAVRTDPDAPP
HKGITMLLVPTSDPGYSCTLINTLASHDTTASYYENIRVPVTRRVGEENKGWRLITNQLN
HERVTLAAHGTMAIRALHDVQRWAMETKLTDGRRVADLPWVRRRLAQTHTRLDAMKLLNW
KMVNAVQEGTLTPQDASAVVVSML

>putative acyl-CoA dehydrogenase [PokU3]
GTGCATCTCGAATACACGCCGGATCAGCAGCGTCTGCGCACCGAACTGCGCGACTACTTC
GCCCAGTTGGTACCCGACAACGCCTACGCCCGGTACGCCGACCCCGCCTCGCAGAAGCGG
TTCTACCGCGAGACCGTCCGGCGCCTGGGCGCGGACGGCTGGCTCGGGGTCGGATGGCCG
AAGGAGTACGGCGGGCGCGGGCTGACCCCCATGGAACAGTTCATCTTCTTCGACGAGGCG
GCCCAGGCGGGCGTGCCCCTGCCCCTGATGGCCCTGAACACCGTCGGCCCGACGATCATG
CAGTTCGGGACGGAGGAACAGAAGTCCCACTTCCTGCCCAGGATCCTCTCCGGCGAGATC
GACTTCGCGATCGGCTACAGCGAGCCCGACGCGGGCACCGACCTCGCCGCGCTGAAGACC
AAGGCGGTCCGCGACGGCGACGGCGACTACGTCGTGAACGGGCAGAAGATCTGGACGACG
AACGGCGACACCGCCGACTGGGTCTGGCTCGCCGTCCGCACCGACCCCGACGCCCCGCCG
CACAAGGGCATCACCATGCTCCTGGTGCCGACCTCCGACCCCGGCTACTCGTGCACGCTG
ATCAACACACTCGCCTCGCACGACACCACCGCCAGCTACTACGAGAACATCCGGGTCCCC
GTCACCCGCCGGGTCGGCGAGGAGAACAAGGGATGGCGGCTGATCACCAACCAGCTCAAC
CACGAACGCGTGACGCTCGCCGCGCACGGCACCATGGCGATCCGGGCCCTGCACGACGTG
CAGCGCTGGGCCATGGAGACCAAGCTCACCGACGGCCGCCGCGTGGCCGACCTCCCGTGG
GTGCGCCGCCGGCTCGCGCAGACGCACACCCGGCTCGACGCCATGAAGCTGCTCAACTGG
AAGATGGTGAACGCCGTCCAGGAGGGCACCCTCACCCCGCAGGACGCCTCCGCGGTGGTC
GTGAGCATGCTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain) PF00441 Acyl-CoA_dh_1 IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain) PF02770 Acyl-CoA_dh_M G3DSA:2.40.110.10 Acyl_CoA_DH/ox_M IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain) PF02771 Acyl-CoA_dh_N IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain) G3DSA:1.20.140.10 AcylCoA_DH_1/2_C SSF47203 AcylCoADH_C_like IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain) SSF56645 AcylCoA_dehyd_NM IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain) G3DSA:1.10.540.10 AcylCoA_DH/ox_N
SignalP	Eukaryota Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Polk_00410 Polk_00430