Sch475_00025 Sch475_00025   Sch475_00040 Sch475_00040

Sch475_00030 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction5,939 / 4,587 / - [in whole cluster]
5,939 / 4,587 / - [in contig]
Locationcomplement(4587..5939) [in whole cluster]
complement(4587..5939) [in contig]
TypeCDS
Length1,353 bp (450 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative major facilitator superfamily transporter
Product (GenBank)putative 3-(2-hydroxyphenyl) propionic acid transporter
Gene
Gene (GenBank)schA3
EC number
Keyword
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchA3(359aa): Putative 3-(2-hydroxyphenyl) propionic acid transporter
Related Reference
ACC
Q45082
PmId
[8828207] ( , )
comment
Blast 208th, id36%, 2e-64
Burkholderia cepacia_mopB
MopB

4-Methylphthalateの異化に関わるmopB。4-methylphthalate and phthalateの取り込みを確認し、基質特異性あり。
ACC
P76350
PmId
[9524262] Cloning and analysis of the shiA gene, which encodes the shikimate transport system of escherichia coli K-12. (Gene. , 1998)
comment
Blast 434th, id34%, 2e-59
E.coli_shiA
Shikimate transporter

Escherichia coli K-12においてシキミ酸の輸送に関与するshiA遺伝子をクローニングしてシークエンス。ShiAはそのアミノ酸配列からMFS superfamilyに属する。shiA破壊株ではシキミ酸の輸送ができなくなった。シキミ酸を芳香環の供与体として添加したときに生育できるaroE strainでshiAを高発現させると、dehydroshikimate (DHS)が蓄積し、シキミ酸の輸送が阻害された（シキミ酸の前駆体であるDHSによってシキミ酸輸送が調節されている）。E. coliではshiAの発現は、芳香族アミノ酸の生合成と輸送に関与する遺伝子群の発現を調節するTyrAでは制御されていないようだった。

close this sectionSequence

selected fasta
>putative major facilitator superfamily transporter [putative 3-(2-hydroxyphenyl) propionic acid transporter]
MSVPAAPLDAPPGQPKKAATAAWIGSALEYYDFFIYGSAAALIFPEVFFDESDPATATLL
SLATFGVAYAARPVGALFLGHFGDKVGRKKIMVFTLILMGLSTFLIGCLPTRDQVGTLAP
VLLVICRVLQGISAAGEQASANSMSLEHAPPNKRGFFTSFTLSGTQGGQLLATLVFIPVA
ALPEDQLLSWGWRVPFWMSIAVAVVGYVIRRTLEETPTFTQQAATEGVAKMPLAVLLRDH
WADVLRVVAAALVASVSTIFTVWALSYATSDSVGMDRTSMLWVGALANLVALGAIPLWAT
LSDRIGRRPVFLIGAAGSGVLMVAYLWAISTGSYPLVMVLGIVTFGVVYSAANGVWPSFY
GEMFSTRVRLSGMAIGTQIGFAIAGFAVTFAAQIAGPDGDDWASVALFTAALCVPPVIAA
LTARETHKVPTEHLGERAPQDSPNREPVSA
selected fasta
>putative major facilitator superfamily transporter [putative 3-(2-hydroxyphenyl) propionic acid transporter]
GTGTCCGTCCCCGCAGCGCCCCTCGACGCGCCACCCGGCCAGCCAAAGAAGGCTGCGACG
GCCGCCTGGATCGGCAGCGCGCTGGAGTACTACGACTTCTTCATCTACGGCAGCGCGGCG
GCGCTGATCTTCCCCGAGGTCTTCTTCGACGAGTCGGACCCGGCCACCGCGACCCTGCTG
TCGCTGGCCACGTTCGGCGTCGCGTACGCGGCCAGGCCGGTCGGCGCGCTGTTCCTCGGG
CACTTCGGCGACAAGGTCGGCCGCAAGAAGATCATGGTCTTCACGCTGATCCTGATGGGC
CTCTCGACGTTCCTCATCGGCTGTCTGCCGACGCGCGACCAGGTCGGCACGCTGGCCCCG
GTGCTCCTGGTGATCTGCCGTGTCCTGCAGGGCATCTCGGCCGCCGGCGAGCAGGCCAGC
GCCAACTCCATGTCACTGGAACACGCGCCACCCAACAAACGCGGCTTCTTCACCAGCTTC
ACGCTCAGCGGCACGCAGGGCGGCCAGCTCCTCGCCACGCTGGTGTTCATCCCGGTCGCC
GCGCTCCCCGAGGACCAACTCCTCTCCTGGGGCTGGCGGGTCCCGTTCTGGATGAGCATC
GCGGTCGCCGTCGTCGGCTACGTCATCCGCCGCACGCTGGAGGAGACCCCGACCTTCACC
CAGCAGGCCGCCACCGAGGGCGTCGCGAAGATGCCGCTCGCCGTGCTGCTGCGGGACCAC
TGGGCGGACGTCCTCAGGGTGGTCGCGGCCGCGCTCGTCGCCTCGGTCAGCACGATCTTC
ACGGTGTGGGCGCTGTCGTACGCCACCAGCGACTCCGTCGGCATGGACCGTACGTCCATG
CTGTGGGTGGGTGCGCTCGCCAACCTGGTCGCCCTGGGCGCCATCCCCCTCTGGGCAACC
CTCTCCGACCGCATCGGCCGCCGGCCCGTCTTCCTGATCGGCGCCGCGGGCAGTGGCGTG
CTGATGGTCGCCTACCTGTGGGCCATCTCCACGGGCTCCTACCCGCTGGTCATGGTCCTC
GGCATCGTCACCTTCGGTGTCGTCTACAGCGCCGCGAACGGTGTCTGGCCGTCCTTCTAC
GGCGAGATGTTCTCCACTCGGGTCCGCCTCTCGGGCATGGCCATCGGCACGCAGATCGGC
TTCGCCATCGCCGGGTTCGCGGTGACCTTCGCCGCGCAGATCGCGGGTCCGGACGGGGAC
GACTGGGCGTCGGTGGCGCTGTTCACCGCGGCGCTGTGCGTCCCGCCGGTGATCGCGGCG
CTCACGGCCCGCGAAACCCACAAGGTCCCGACGGAACACCTCGGCGAGCGGGCACCCCAG
GACTCCCCGAACCGAGAGCCGGTCTCGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005828 General substrate transporter (Family)
[20-225] 6.8e-28 PF00083 [247-433] 3.5e-14 PF00083
PF00083   Sugar_tr
IPR016196 Major facilitator superfamily domain, general substrate transporter (Domain)
[1-439] 1.2e-65 SSF103473
SSF103473   MFS general substrate transporter
IPR020846 Major facilitator superfamily domain (Domain)
[18-428] 32.518 PS50850
PS50850   MFS
SignalP
[1-21] 0.287 Signal
Bacteria, Gram-positive   
TMHMM
[21-43] Transmembrane (i-o) [58-80] Transmembrane (o-i) [92-111] Transmembrane (i-o) [190-209] Transmembrane (o-i) [244-265] Transmembrane (i-o) [280-302] Transmembrane (o-i) [309-331] Transmembrane (i-o) [335-357] Transmembrane (o-i) [370-392] Transmembrane (i-o) [402-421] Transmembrane (o-i)
Transmembrane 10   
Sch475_00025 Sch475_00025   Sch475_00040 Sch475_00040
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