Sch475_00040 Sch475_00040   Sch475_00060 Sch475_00060

Sch475_00050 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction7,072 / 7,965 / + [in whole cluster]
7,072 / 7,965 / + [in contig]
Location7072..7965 [in whole cluster]
7072..7965 [in contig]
TypeCDS
Length894 bp (297 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative quinate/shikimate dehydrogenase
putative NAD(+)-dependent quinate dehydrogenase
Product (GenBank)putative shikimate 5-dehydrogenase
Gene
Gene (GenBank)schA5
EC number1.1.1.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchA5(297aa): Shikimate 5-dehydrogenase

本文にコメントなし。
Related Reference
ACC
Q9X5C9
PmId
comment
Blast 112th, id50%, 4e-55
Corynebacterium glutamicum_aroE
Quinate/shikimate dehydrogenase(EC 1.1.1.24/1.1.1.-)
synonym: NAD(+)-dependent quinate dehydrogenase

3つの異なる機能的クラスの細菌shikimate/quinate DHがこれまでに同定されている。
AroE --基質：shikimate、補酵素：NADP+
SDH-L --AroEと同じ。反応がAroEより遅い。
YdiB --基質：shikimate/quinate、補酵素：NAD+/NADP+
これら3つのsubclassは類似した3次元折りたたみを持ち、それゆえすべてが同じ構造クラスのタンパクに属する。

この論文で解析されているCorynebacterium glutamicumの酵素は、
基質：quinate >> shikimate
補酵素：NAD+
タンパクの3次元構造は上記3つのsubclassのメンバーに似ているので、shikimate/quinate DH familyの新しい機能的classに属する。

さらに結晶構造解析をして特異性の違いの理由を推測している。

close this sectionSequence

selected fasta
>putative quinate/shikimate dehydrogenase [putative shikimate 5-dehydrogenase]
MVKDSYLVGLIGAGIGPSLSPALHEREADRQGLRYLYRLIDIDALGVGPQAVGDLVRAAR
DLGFDGLNITHPCKQLVIGHLDALAPQAEALGAVNTVVFEGGRAVGHNTDVTGFAASFAR
GLPDAPLERVVQLGAGGAGAAVAHAMLTLGAGHVTVVDAMPDRSADLAASLNRHFGAGRA
AAAGPERLAALLGGADGIVHATPTGMAAHPGLPLPGELLHPGLWVAEVVYRPLETELLRA
ARAAGCAVLDGGGMAVFQAADAFRLFTGREPDAVRMLADIAELAGVAGAAGAAGARS
selected fasta
>putative quinate/shikimate dehydrogenase [putative shikimate 5-dehydrogenase]
ATGGTCAAGGACTCGTATCTCGTCGGGCTGATCGGCGCCGGGATCGGCCCGTCGCTCAGC
CCGGCACTGCACGAGCGGGAGGCCGACCGGCAGGGCCTGCGCTATCTGTACCGGCTGATC
GACATCGACGCGCTCGGTGTCGGGCCGCAGGCGGTGGGGGACCTCGTACGAGCCGCCCGC
GACCTGGGCTTCGACGGGCTGAACATCACGCATCCCTGCAAGCAGCTCGTCATCGGGCAT
CTGGACGCGCTCGCCCCGCAGGCCGAGGCGCTCGGCGCGGTGAACACCGTCGTCTTCGAG
GGCGGGCGTGCGGTCGGGCACAACACCGATGTCACCGGGTTCGCCGCCTCGTTCGCCCGT
GGGCTGCCGGATGCCCCGCTGGAGCGGGTCGTGCAGTTGGGCGCGGGGGGAGCGGGGGCG
GCCGTCGCGCATGCCATGCTCACGCTCGGGGCCGGGCACGTCACCGTCGTCGATGCCATG
CCGGACCGGTCGGCGGACCTCGCCGCCTCGCTGAACCGGCACTTCGGTGCGGGGCGGGCC
GCTGCCGCGGGCCCGGAGCGGCTGGCGGCGCTGCTCGGCGGTGCGGACGGCATCGTGCAT
GCCACGCCGACGGGGATGGCCGCTCATCCGGGGCTGCCGCTTCCCGGTGAGTTGCTGCAT
CCCGGGTTGTGGGTGGCCGAGGTGGTGTACCGGCCGTTGGAGACCGAGTTGCTGCGTGCC
GCTCGGGCGGCGGGGTGTGCGGTTCTCGATGGTGGGGGGATGGCTGTTTTCCAGGCCGCG
GACGCGTTTCGGCTGTTCACGGGGCGGGAGCCGGACGCGGTGCGGATGCTTGCGGATATT
GCGGAGTTGGCCGGTGTGGCCGGTGCCGCAGGCGCCGCCGGGGCGCGGAGTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR013708 Shikimate dehydrogenase substrate binding, N-terminal (Domain)
[10-97] 5.5e-22 PF08501
PF08501   Shikimate_dh_N
IPR016040 NAD(P)-binding domain (Domain)
[115-279] 5.69999999999998e-43 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Sch475_00040 Sch475_00040   Sch475_00060 Sch475_00060
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